Effect of Ultrasonic Treatment on the Bactericidal Effect of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> in Goat Milk

REN Rong; ZHANG Anqi; ZHANG Fuxin; LIU Yufang; WANG Weizhe; HE Chao; LIU Yingying; WANG Bini

doi:10.13386/j.issn1002-0306.2020120270

Volume 42 Issue 18

Sep. 2021

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Science and Technology of Food Industry > 2021 > 42(18): 126-133. > DOI: 10.13386/j.issn1002-0306.2020120270

REN Rong, ZHANG Anqi, ZHANG Fuxin, et al. Effect of Ultrasonic Treatment on the Bactericidal Effect of Escherichia coli and Staphylococcus aureus in Goat Milk[J]. Science and Technology of Food Industry, 2021, 42(18): 126−133. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120270.

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Effect of Ultrasonic Treatment on the Bactericidal Effect of Escherichia coli and Staphylococcus aureus in Goat Milk

College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, China

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Received Date: December 29, 2020
Available Online: July 15, 2021

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Abstract

The bactericidal effect of ultrasonic treatment on Escherichia coli and Staphylococcus aureus in fresh goat milk were studied in this paper, which provided reference for the non-thermal sterilization method and product development of goat milk. Based on a single factor experiment, the Box-Behnken experimental design was used to study the effects of ultrasonic power, temperature and time on the sterilization effect of Escherichia coli and Staphylococcus aureus in goat milk, and the sterilization logarithm of E. coli and S. aureus was taken as response value, the ultrasonic processing conditions were optimized through response surface analysis, and also studied the destruction of bacterial cells of the two strains by ultrasonic and pasteurization treatment and the changes of colony numbers during storage period. The results showed that the optimal conditions were ultrasonic power 530 W, temperature 60 ℃, and time 30 min. Under these conditions, the sterilization logarithms values for E. coli and S. aureus were 7.55 and 6.53. Compared with pasteurization, ultrasonic treatment had grater sterilization effect that could destroy the cell surface structure of E. coli and S. aureus to a greater extent, and extended the storage period of goat milk from 14 d to 21 d after pasteurization, and the quality requirements still met national standards.
- ultrasound treatment,
- goat milk,
- conditional pathogen,
- Escherichia coli,
- Staphylococcus aureus,
- optimization of sterilization conditions

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