Protective Effect of Curcumin/Cyclodextrin Polymer Inclusion Complex on LO2 Cells Damaged by Ethanol

FAN Tugui; CHEN Jianping; GAO Jialong; ZHONG Saiyi; QIN Xiaoming

doi:10.13386/j.issn1002-0306.2020120083

Volume 42 Issue 18

Sep. 2021

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Science and Technology of Food Industry > 2021 > 42(18): 366-371. > DOI: 10.13386/j.issn1002-0306.2020120083

FAN Tugui, CHEN Jianping, GAO Jialong, et al. Protective Effect of Curcumin/Cyclodextrin Polymer Inclusion Complex on LO2 Cells Damaged by Ethanol[J]. Science and Technology of Food Industry, 2021, 42(18): 366−371. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120083.

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Protective Effect of Curcumin/Cyclodextrin Polymer Inclusion Complex on LO2 Cells Damaged by Ethanol

FAN Tugui^{1, 2,},
CHEN Jianping^{1, 2, ,},
GAO Jialong^{1, 2},
ZHONG Saiyi^{1, 2},
QIN Xiaoming^{1, 2}

1.
College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524088, China
2.
Dalian Polytechnic University, Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China

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Received Date: December 08, 2020
Available Online: July 19, 2021

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Abstract

Abstract

This study investigated the protective effect of curcumin/cyclodextrin polymer inclusion complex(CUR/CDP) on LO2 cells damaged by ethanol. The MTT method was used to establish a model of LO2 cells damaged by ethanol. Glutamate alanine aminotransferase(ALT), aspartate aminotransferase(AST), lactate dehydrogenase(LDH), superoxide dismutase(SOD), glutathione peroxidase(GSH-PX), malondialdehyde(MDA) and reactive oxygen species(ROS) kits were used to investigate the protective effect of CUR/CDP on LO2 cells damaged by ethanol. The results showed that after CUR/CDP (80 μg/mL) treatment, the cell activity of LO2 cells increased from (54.75%±8.97%) (model group) to 85.27%±2.64%, and the activities of ALT, AST, and LDH in the LO2 cell culture medium decreased from (26.47±0.90), (41.02±4.41), (63.77±4.95) U/L (model group) to (16.17±0.42), (22.62±0.79), (32.25±1.69) U/L (P<0.01), respectively; the activities of GSH-PX and SOD in LO2 cells increased from (21.82±1.34), (8.45±1.11) U/mg prot (model group) to (36.70±0.56), (16.47±1.27) U/mg prot; the content of MDA and ROS in LO2 cells decreased from (1.19±0.15) nmol/mg prot, (198.02%±11.76%) (model group) to (0.72±0.05) nmol/mg prot, (110.87%±10.22%) (P<0.01), respectively. In conclusion, CUR/CDP could improve the damage of LO2 cells induced by ethanol by increasing the activity of antioxidant enzymes and reducing the content of ROS.
- curcumin/cyclodextrin polymer inclusion complex,
- LO2 cells,
- ethanol-induced injury,
- protective effect,
- reactive oxygen species

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References

[1]	Li J, Yan H T, Che J X, et al. Effects of neurolytic celiac plexus block on liver regeneration in rats with partial hepatectomy[J]. Medical Journal of National Defending Forces in Southwest China,2013,8(9):73101−73109.
[2]	左军, 唐明哲, 韩淑丽, 等. 中医药治疗酒精性肝损伤的研究进展[J]. 中医药信息,2017,34(3):124−128. [Zuo J, Tang M Z, Han S L, et al. Research progress on treatment of alcoholic liver injury with traditional Chinese medicine[J]. Information on Traditional Chinese Medicine,2017,34(3):124−128. doi: 10.3969/j.issn.1002-2406.2017.03.036
[3]	曲航, 高鑫, 伊娟娟, 等. 食源性天然产物对酒精性肝损伤的防护作用研究进展[J]. 食品科学,2020,41(17):283−290. [Qu H, Gao X, Yi J J, et al. Review on the protective effects of food-derived natural compounds on alcohol-induced liver injury[J]. Food Science,2020,41(17):283−290. doi: 10.7506/spkx1002-6630-20190920-262
[4]	Chen X, Zou L Q, Niu J, et al. The stability, sustained release and cellular antioxidant activity of curcumin nanoliposomes[J]. Molecules,2015,20(8):14293−14311. doi: 10.3390/molecules200814293
[5]	Ye F Y, Lei D D, Wang S M, et al. Polymeric micelles of octenylsuccinated corn dextrin as vehicles to solubilize curcumin[J]. LWT-Food Science and Technology,2017,75(1):187−194.
[6]	Fu Y M, Zheng S Z, Lin J G, et al. Curcumin protects the rat liver from CCl₄-caused injury and fibrogenesis by attenuating oxidative stress and suppressing inflammation[J]. Molecular Pharmacology February,2008,73(2):399−409.
[7]	Nanji A A, Ainenk A J, Tipoe G L, et al. Curcumin prevents alcohol-induced liver disease in rats by inhibiting the expression of NF-kB-dependent genes[J]. American Journal of Physiology-Gastrointestinal and Liver Physiology,2003,284(2):321−327. doi: 10.1152/ajpgi.00230.2002
[8]	Cremers N A J, Lundvig Ditte M S, Van Dalen S C M, et al. Curcumin-induced heme oxygenase-1 expression prevents H₂O₂-induced cell death in wild type and heme oxygenase-2 knockout adipose-derived mesenchymal stem cells[J]. International Journal of Molecular Sciences,2014,15(10):17974−17999. doi: 10.3390/ijms151017974
[9]	张媛媛, 宋理平, 郭辉, 等. 姜黄素对四氯化碳诱导鲤肝脏损伤的修复作用[J]. 广东海洋大学学报,2020,40(5):1−11. [Zhang Y Y, Song L P, Guo H, et al. Research of curcumin on recovery effect of liver injury in Cyprinus carpio induced by carbon tetrachloride[J]. Journal of Guangdong Ocean University,2020,40(5):1−11. doi: 10.3969/j.issn.1673-9159.2020.05.001
[10]	郭依依. 姜黄素对急性高脂血症大鼠肝脏氧化应激及Nrf2/HO-1信号通路的影响[D]. 石家庄: 河北医科大学, 2016. Guo Y Y. The influence of curcumin on the level of hepatic oxidative stress and Nrf2/HO-1 signaling pathway on acute hyperlipidemia rats[D]. Shijiazhuang: Hebei Medical University, 2016.
[11]	黄汉昌, 路书彦, 常平, 等. Nrf2-ARE信号通路参与姜黄素对Aβ诱导细胞氧化损伤和凋亡的抑制作用研究[J]. 现代食品科技,2015,31(6):1−5. [Huang H C, Lu S Y, Chang P, et al. Participation of Nrf2-ARE signaling in curcumin inhibition of Amyloid-β-induced cellular oxidative stress and cell apoptosis[J]. Modern Food Science and Technology,2015,31(6):1−5.
[12]	Chen J P, Qin X M, Zhong S Y, et al. Characterization of curcumin/cyclodextrin polymer inclusion complex and investigation on its antioxidant and antiproliferative activities[J]. Molecules,2018,23(6):1179.
[13]	陈建平, 彭莞仪, 秦小明, 等. 姜黄素超分子包合物的结构鉴定及其抗氧化活性[J]. 食品工业科技,2017,38(21):21−25. [Chen J P, Peng G Y, Qin X M, et al. Structural identification of curcumin/β-cyclodextrin polymer inclusion complex and its antioxidant activity[J]. Science and Technology of Food Industry,2017,38(21):21−25.
[14]	陈建平, 钟赛意, 秦小明, 等. 负载姜黄素β-环糊精功能化纳米银诱导HepG2细胞凋亡机制[J]. 广东海洋大学学报,2019,39(1):78−83. [Chen J P, Zhong S Y, Qin X M, et al. Preliminary study on the molecular mechanism of cyclodextrin functional silver nanoparticles-loaded curcumin induced HepG2 cells apoptosis[J]. Journal of Guangdong Ocean University,2019,39(1):78−83.
[15]	Teschke R. Alcoholic liver disease: Alcohol metabolism, cascade of molecular mechanisms, cellular targets, and clinical aspects[J]. Biomedicines,2018,6(4):106−162. doi: 10.3390/biomedicines6040106
[16]	Levene A P, Goldin R D. The epidemiology, pathogenesisand histopathology of fatty liver disease[J]. Histopathol,2012,61(2):141−52. doi: 10.1111/j.1365-2559.2011.04145.x
[17]	Sheikhzadeh N, Tayefi-Nasrabadi H, Oushani A K, et al. Effects of Haematococcus pluvialis supplementation on antioxidant system and metabolism in rainbow trout(Oncorhynchus mykiss)[J]. Fish Physiology and Biochemistry,2012,38(2):413−419. doi: 10.1007/s10695-011-9519-7
[18]	曾瑜, 刘婧, 黄真真, 等. 姜黄素对急性酒精性肝损伤小鼠抗氧化功能的影响[J]. 卫生研究,2014,43(2):282−285. [Zeng Y, Liu J, Huang Z Z, et al. Effect of curcumin on antioxidant function in the mice with acute alcoholic liver injury[J]. Journal of Hygiene Research,2014,43(2):282−285.
[19]	李珂. 姜黄素对酒精诱导的肝细胞损伤的保护作用及其机制研究[D]. 武汉: 华中科技大学, 2006. Li K. The protective effect of curcumin against ethanol-induced hepatocyte oxidative damage: Involvement of HO-1/Nrf2 pathway[D]. Wuhan: Huazhong University of Science and Technology, 2006.
[20]	许丽璇, 蔡建秀, 岳枝玲. 桦褐孔菌乙醇提取物对小鼠体内外抗氧化作用研究[J]. 中国食品学报,2012,12(6):48−55. [Xu L X, Cai J X, Yue Z L. Study on antioxidant effect of ethanol extract of Inonotus obliquus on mice[J]. Journal of Chinese Institute of Food Science and Technology,2012,12(6):48−55. doi: 10.3969/j.issn.1009-7848.2012.06.008
[21]	刘春红, 金钟斗, 韩宝瑞. 平贝母多糖对D-半乳糖诱导衰老模型小鼠的抗氧化作用[J]. 食品科学,2011,32(23):285−288. [Liu C H, Kim J D, Hang B R. Antioxidant activity of polysaccharide FUP-1 from Fritillaria ussuriensis maxim in D-galactose-induced aging mouse model[J]. Food Science,2011,32(23):285−288.
[22]	袁列江, 李忠海, 郑锦星, 等. 槟榔提取物对小白鼠体内抗氧化作用的研究[J]. 食品科学,2009,30(7):225−228. [Yuan L J, Li Z H, Zheng J X, et al. In vitro and in vivo antioxidant activities of Varous areca nut extracts[J]. Food Science,2009,30(7):225−228. doi: 10.3321/j.issn:1002-6630.2009.07.051
[23]	郭增旺, 樊乃境, 田海芝, 等. 小米糠黄酮对H₂O₂致HepG2氧化应激损伤的保护作用[J]. 食品科学,2020,41(5):159−165. [Guo Z W, Fan N J, Tian H Z, et al. Protective effects of flavonoids from millet bran on H₂O₂-induced oxidative stress injury in HepG2 cells[J]. Food Science,2020,41(5):159−165. doi: 10.7506/spkx1002-6630-20190101-013
[24]	Pan J H, Lee K Y, Kim J H, et al. Prunus mume Sieb. et Zucc. fruit ameliorates alcoholic liver injury in mice by inhibiting apoptosis and inflammation through oxidative stress[J]. Journal of Functional Foods,2016,25(1):135−148.
[25]	Tang J Y, Cao L, Li Q, et al. Selenoprotein x gene knockdown aggravated H₂O₂-induced apoptosis in liver LO2 cells[J]. Biological Trace Element Research,2016,173(1):71−78. doi: 10.1007/s12011-016-0653-z
[26]	Xia T, Yao J H, Zhang J, et al. Protective effects of Shanxi aged vinegar against hydrogen peroxide-induced oxidativedamage in LO2 cells through Nrf2-mediated antioxidant responses[J]. Royal Society of Chemistry Advances,2017,7(28):17377−17386.
[27]	Tang J Y, He A H, Jia G L, et al. Protective effect of selenoprotein x against oxidative stress-induced cell apoptosis in human hepatocyte(LO2) cells via the p38 pathway[J]. Biological Trace Element Research,2018,181(1):44−53. doi: 10.1007/s12011-017-1025-z

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