Optimization of Fermentation Medium for <i>γ</i>-Polyglutamic Acid Production by <i>Bacillus siamese</i> LW-1

CAI Yahui; WANG Qing; WANG Wenyu; HUANG Gaofeng; ZHANG Jiran; XU Shuxia; ZHANG Shimin; WU Kun

doi:10.13386/j.issn1002-0306.2020110269

Volume 42 Issue 16

Aug. 2021

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Science and Technology of Food Industry > 2021 > 42(16): 163-170. > DOI: 10.13386/j.issn1002-0306.2020110269

CAI Yahui, WANG Qing, WANG Wenyu, et al. Optimization of Fermentation Medium for γ-Polyglutamic Acid Production by Bacillus siamese LW-1 [J]. Science and Technology of Food Industry, 2021, 42(16): 163−170. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110269.

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Optimization of Fermentation Medium for γ-Polyglutamic Acid Production by Bacillus siamese LW-1

College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China

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Received Date: November 29, 2020
Available Online: June 16, 2021

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Abstract

Abstract

In order to increase the production of γ-polyglutamic acid (γ-PGA) from Bacillus siamese LW-1, three significant factors affecting yield of γ-PGA from the initial fermentation medium components were screened through the Plackett-Burman(PB) and the steepest climbing experiment based on the single-factor experiment to determine the optimal area of the response surface; the optimal medium components of Bacillus siamese LW-1 were obtained by using a three-factor and three-level Box-Behnken experiment. The optimal medium components for Bacillus siamese LW-1 producing γ-PGA were: sodium glutamate 86.71 g/L, sodium citrate 17.94 g/L, MgSO₄·7H₂O 2.11 g/L, glycerol 25 g/L, KH₂PO₄ 1.4 g/L, (NH₄)₂SO₄ 14 g/L, MnSO₄ 0.075 g/L, CaCl₂ 0.1 g/L, FeCl₃·6H₂O 0.04 g/L. Under this optimum condition, the yield of γ-PGA was 44.78 g/L which was 1.93 times that of before optimization(23.26 g/L ), and it was very close to the maximum value 45.91 g/L predicted by the theory.
- Bacillus siamese,
- γ-polyglutamic acid,
- response surface methodology,
- Plackett-Burman test,
- fermentation medium

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References

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