Study on the Preparation of Polysaccharide Lozenge from <i>Morchella esculenta</i> and Its Antioxidant Activity

WANG Xuemei; LONG Yefeng; LIN Yongtao; ZHOU Chusheng; KONG Yongxin; WU Siying; ZHOU Chunhui

doi:10.13386/j.issn1002-0306.2020110210

Volume 42 Issue 19

Sep. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(19): 163-172. > DOI: 10.13386/j.issn1002-0306.2020110210

WANG Xuemei, LONG Yefeng, LIN Yongtao, et al. Study on the Preparation of Polysaccharide Lozenge from Morchella esculenta and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2021, 42(19): 163−172. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110210.

Citation:

PDF (2808 KB)

Study on the Preparation of Polysaccharide Lozenge from Morchella esculenta and Its Antioxidant Activity

WANG Xuemei^{1, 2,},
LONG Yefeng^{1, 2},
LIN Yongtao^{1, 2},
ZHOU Chusheng^{1, 2},
KONG Yongxin^{1, 2},
WU Siying^{1, 2},
ZHOU Chunhui^1, ,

1.
School of Food and Biotechnology, Guangdong Institute of Light Industry, Guangzhou 510030, China
2.
College of Biology and Food Engineering, Guangdong Second Normal University, Guangzhou 510303, China

More Information

Received Date: November 22, 2020
Available Online: August 11, 2021

Graphical Abstract

Abstract

Abstract

With the amniotic bacteria sub-entity as the main raw material, by cellulase, papaya protease complex enzyme solution, ultrasound assisted extraction to obtain Morchella esculenta polysaccharides, further through the addition of auxiliary ingredients to develop amniotic bacteria polysaccharide tablets. Single-factor and response surface experimental optimization obtained the best conditions for the optimal extraction of Morchella esculenta polysaccharides for liquid ratio of 29:1 mL/g, enzymatic pH5.1, enzymatic time 2 h, enzyme dosage 1.4%, ultrasonic time 20 min, the rate of extraction reached up to 9.21%; Single-factor experiments and orthogonal experiments determined that the best type of filler for polysaccharide tablets of amniotic bacteria was glycol:sorbitol: 1:1, the composition of the ingredients as fillers and polysaccharide leaching paste ratio was 8:1, vitamin C was added 5 mg/g, the addition of stingric acid magnesium was 0.8%, and the comprehensive score of the tablets was 86.4 under this condition. When the tablet concentration was 10 mg/mL, the antioxidant activity results were: hydroxy radical clearance of 46.78% and DPPH radical clearance of 75.38%, with good antioxidant activity.
- Morchella esculenta polysaccharides,
- extraction process optimization,
- tablet formulation,
- antioxidant activity

FullText(HTML)

References (26)

References

[1]	李卫东. 羊肚菌生物活性成分及药理作用的研究进展综述[J]. 食药用菌,2018,26 (3):157−160. [Li Weidong. Review of the bioactive ingredients and pharmacological action of Morchella[J]. Edible and Medicinal Mushrooms,2018,26 (3):157−160.
[2]	矫丽曼. 羊肚菌胞内多糖的提取及单糖组成分析[J]. 西部林业科学,2016,45 (4):130−135. [Jiao Liman. Extraction of intracellular polysaccharides and monosaccharide composition analysis of Morchella[J]. Journal of West China Forestry Science,2016,45 (4):130−135.
[3]	肖宝石, 吕海涛. 酶法提取浒苔多糖工艺优化的研究[J]. 食品与机械,2010,26 (5):125−127. [Xiao Baoshi, Lv Haitao. Study on optimization of polysaccharide extraction by enzymatic method[J]. Food and Machinery,2010,26 (5):125−127.
[4]	Xu Na, Sun Yonghai, Guo Xiaolei , et al. Optimization of ultrasonic−microwave synergistic extraction of polysaccharides from Morchella conica[J]. Journal of Food Processing and Preservation,2018,42(2).
[5]	董宇, 林翰清, 缪松, 等. 酶法提取多糖的研究进展[J]. 食品工业科技,2021,42 (3):351−358. [Dong Yu, Lin Hanqing, Miao Song, et al. Progress in the extraction of polysaccharides by enzymatic method[J]. Science and Technology of Food Industry,2021,42 (3):351−358.
[6]	李蔚, 王忠民, 龚平, 等. 超声波提取羊肚菌菌丝体多糖的研究[J]. 农产品加工(学刊),2008(3):25−28. [Li Wei, Wang Zhongmin, Gong Ping, et al. Study on ultrasonic extraction of Morchella mycelium polysaccharide[J]. Academic Periodical of Farm Products Processing,2008(3):25−28.
[7]	闫永兰. 酶提羊肚菌子实体多糖的抗氧化和抗衰老作用[J]. 中国食用菌,2020,39 (1):42−45, 60. [Yan Yonglan. Antioxidative and anti-aging effects of polysaccharide[J]. Edible Fungi of China,2020,39 (1):42−45, 60.
[8]	韩融冰, 赵玉卉, 路等学, 等. 羊肚菌多糖提取工艺研究[J]. 北方园艺,2018(23):150−155. [Han Rongbing, Zhao Yuhui, Lu Dengxue, et al. Study on the extraction technology of Morchella polysaccharides[J]. Northern Horticulture,2018(23):150−155.
[9]	付天宇. 基于深层发酵的羊肚菌功能性食品研究[D]. 长春: 吉林大学, 2013. Fu Tianyu. Study on Morchella functional foods based on deep fermentation[D]. Changchun: Jilin University, 2013.
[10]	刘超. 液体深层发酵羊肚菌胞内多糖提取、结构分析及抗结肠癌作用研究[D]. 长春: 吉林大学, 2017. Liu Chao. The intracellular polysaccharide extraction, structural analysis and effect of liquid deep fermentation Morchella against colon cancer[D]. Changchun: Jilin University, 2017.
[11]	田雨. 羊肚菌蛋白质提取及抗氧化肽制备研究[D]. 太原: 山西大学, 2020. Tian Yu. Morchella protein extraction and preparation of antioxidant peptides[D]. Taiyuan: Shanxi University, 2020.
[12]	陈超, 岳崇慧, 王艳菲, 等. 超声波辅助酶法提取榛蘑多糖[J]. 食品工业科技,2017,38 (8):222−226, 255. [Chen Chao, Yue Chonghui, Wang Yanfei, et al. Extraction of Hazelnut mushroom polysaccharide by ultrasonic-assisted enzyme method[J]. Science and Technology of Food Industry,2017,38 (8):222−226, 255.
[13]	Wang Long, LiuYing, PuFan, et al. Effect of rotary evaporator water bath temperature on recovery rate of phthalate esters[J]. Bulletin of Environmental Contamination and Toxicology,2018,101(6):810−813. doi: 10.1007/s00128-018-2440-3
[14]	程振玉, 杨英杰, 刘治刚. 超声波辅助酶法提取北五味子多糖工艺研究[J]. 中国酿造,2014,33 (3):104−108. [Cheng Zhenyu, Yang Yingjie, Liu Zhigang. Study on the extraction of polysaccharides by ultrasonic assisted enzyme[J]. Chinese brewing,2014,33 (3):104−108. doi: 10.3969/j.issn.0254-5071.2014.03.025
[15]	Zhou Yong Dong; Hua Hua Wang; Ming Yue Li, et al. Optimization of high-intensity pulsed electric field-assisted extraction of procyanidins from Vitis amurensis seeds using response surface methodology[J]. E3S Web of Conferences,2020,189:02029. doi: 10.1051/e3sconf/202018902029
[16]	Tang Yun, Zhu Zhen-Yuan, Pan Li-Chao, et al. Structure analysis and anti-fatigue activity of a polysaccharide from Lepidium meyenii Walp.[J]. Natural product research,2019,33(17):2480−2489. doi: 10.1080/14786419.2018.1452017
[17]	刘梁, 孙维矿, 赵玲, 等. 米糠多糖口含片的制备工艺[J]. 食品研究与开发,2015,36 (8):46−49. [Liu Liang, Sun Weikuang, Zhao Ling, et al. Preparation process of rice bran polysaccharide mouth tablets[J]. Food Research and Development,2015,36 (8):46−49. doi: 10.3969/j.issn.1005-6521.2015.08.013
[18]	郑小亮, 祝丽华, 沈孟宏, 等. 香菇多糖的提取纯化及颗粒剂制备的处方优化[J]. 黑龙江八一农垦大学学报,2019,31 (1):68−73. [Zheng Xiaoliang, Zhu Lihua, Shen Menghong, et al. Extraction and purification of shiitake polysosaccharides and prescription optimization of granule preparation[J]. Journal of Heilongjiang Bayi Agricultural Reclamation University,2019,31 (1):68−73. doi: 10.3969/j.issn.1002-2090.2019.01.012
[19]	黄庆斌, 卢旭. 竹荪多糖口含片生产工艺研究[J]. 现代食品,2019(18):105−109. [Huang Qingbin, Lu Xu. Study on production process of bamboo sun[J]. Modern food,2019(18):105−109.
[20]	佟晔, 丁雪鹰, 高静, 等. 中药口崩片体外崩解时限测定方法的研究[J]. 第二军医大学学报,2009,30 (7):846−849. [Tong Ye, Ding Xueying, Gao Jing, et al. Study on determination of time limit of Chinese medicine oral collapse tablets[J]. Journal of the Second Military Medical University,2009,30 (7):846−849.
[21]	Opa Vajragupta, Preecha Boonchoong, Lawrence J. Berliner. Manganese complexes of curcumin analogues: Evaluation of hydroxyl radical scavenging ability, superoxide dismutase activity and stability towards hydrolysis[J]. Free Radical Research,2004,38(3):303−314. doi: 10.1080/10715760310001643339
[22]	Ma C W, Feng M Y, Zhai X F, et al. Optimization for the extraction of polysaccharides from Ganoderma lucidum and their antioxidant and antiproliferative activities[J]. Journal of the Taiwan Institute of Chemical Engineers,2013,44(6):886−894. doi: 10.1016/j.jtice.2013.01.032
[23]	王锐, 张泽俊, 熊汝琴. 肾茶多糖纤维素酶法提取工艺及抗氧化活性研究[J]. 食品研究与开发,2020,41 (13):145−151. [Wang Rui, Zhang Zejun, Xiong Ruqin. Extraction process and antioxidant activity of polysoglylase in renal tea[J]. Food Research and Development,2020,41 (13):145−151. doi: 10.12161/j.issn.1005-6521.2020.13.023
[24]	吴昊, 司月阳, 王珊珊, 等. 响应面法优化红菇多糖的提取工艺研究[J]. 粮食与油脂,2020,33(9):90−94. [Wu Hao, Si Yueyang, Wang Shanshan, et al. Study on extraction technology of red mushroom polysaccharide by response surface method[J]. Food and Grease,2020,33(9):90−94. doi: 10.3969/j.issn.1008-9578.2020.09.021
[25]	Kumar Ankit, Srinivasa Rao Pavuluri. Optimization of pulsed-mode ultrasound assisted extraction of bioactive compounds from pomegranate peel using response surface methodology[J]. Journal of Food Measurement and Characterization,2020,14(6):3493−3507. doi: 10.1007/s11694-020-00597-9
[26]	Anguita Ruiz Augusto, Aguilera Concepción M, GilÁngel. Genetics of lactose intolerance: An updated review and online interactive world maps of phenotype and genotype frequencies[J]. Nutrients,2020,12(9):2689. doi: 10.3390/nu12092689