Effects of Rice Bran Protein on Lipid Metabolism and Its Mechanism in Rats

WANG Jilite; YONG Yaping; SU Jing; XIA Meiru; LIU Cong

doi:10.13386/j.issn1002-0306.2020110181

Volume 42 Issue 19

Sep. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(19): 363-368. > DOI: 10.13386/j.issn1002-0306.2020110181

WANG Jilite, YONG Yaping, SU Jing, et al. Effects of Rice Bran Protein on Lipid Metabolism and Its Mechanism in Rats[J]. Science and Technology of Food Industry, 2021, 42(19): 363−368. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110181.

Citation:

PDF (2230 KB)

Effects of Rice Bran Protein on Lipid Metabolism and Its Mechanism in Rats

Department of Agriculture, Hetao College, Bayannur 015000, China

More Information

Received Date: November 19, 2020
Available Online: July 29, 2021

Graphical Abstract

Abstract

Abstract

This study investigated the effects of rice bran protein on lipid metabolism and its mechanism in rats fed with high-cholesterol diet. The experiment designed 4 groups including casein group (CAS, control), rice bran protein group(RBP), brown rice protein group (BRP) and rice protein group(WRP). The rats were fed with high-cholesterol diet supplemented with 20% casein, 15% casein+5% rice bran protein, 15% casein+5% brown rice protein and 15% casein+5% rice protein for 3 weeks. The levels of total cholesterol (TC) and contents of triglyceride (TG) in serum, and TC, TG and total lipid in liver of rats were measured. The feces were collected for 3 days before the animals were sacrificed, and the excretion of total bile acids and neutral sterols were determined. The micellar solubility of cholesterol with CAS, RBP, BRP and WRP, and the abilities of different concentrations CAS and RBP to bind with bile acid were determined by simulating human gastrointestinal environment in vitro. The results showed that compared with casein and other protein groups, rice bran protein significantly reduced serum TC (P<0.05) and atherosclerotic index (P<0.05); liver TC level was significantly reduced (P<0.05) and the total bile acid excretion in feces was significantly increased (P<0.05). The in vitro experiments showed that rice bran protein could bound to bile acids and significantly inhibited the solubility of cholesterol micelles (P<0.05). To sum up, rice bran protein bound to bile acids in vivo to inhibit its absorption in the intestines and increase its excretion in feces, and finally achieved the effects of reducing serum lipids.
- rice bran protein,
- micelles,
- bile acid,
- hyperlipidemia

FullText(HTML)

References (26)

References

[1]	Arata Banno, Jilite Wang, Kenji Okada, et al. Identifcation of a novel cholesterol-lowering dipeptide, phenylalanine-proline (FP), and its down-regulation of intestinal ABCA1 in hypercholestero- lemic rats and Caco-2 cells[J]. Scientific Reports,2019,9:19416. doi: 10.1038/s41598-019-56031-8
[2]	王凤玲, 隋小芳, 索树珍, 等. 胆固醇代谢紊乱及其相关疾病的研究[J]. 中国老年保健医学,2015,13(1):13−15. [Wang Fengling, Sui Xiaofang, Suo Shuzhen, et al. Research on cholesterol metabolism disorders and related diseases[J]. Chinese Journal of Geriatric Carey,2015,13(1):13−15. doi: 10.3969/j.issn.1672-4860.2015.01.003
[3]	Zhong F, Liu J M, Ma J G, et al. Preparation of hypocholesterol peptides from soy protein and their hypocholesterolemic effect in mice[J]. Food Res Int,2007,40:661−667. doi: 10.1016/j.foodres.2006.11.011
[4]	Seyed M N, Shima M S, Javad A S, et al. Soy, soy isoflavones, and protein intake in relation to mortality from all causes, cancers, and cardiovascular diseases: A systematic review and dose-response meta-analysis of prospective cohort studies[J]. Journal of the Academy of Nutrition and Dietetics,2019,119(9):1483−1500. doi: 10.1016/j.jand.2019.04.011
[5]	Cristina M, Justo P, María del MarYust, et al. Sunflower protein hydrolysates reduce cholesterol micellar solubility[J]. Plant Foods Hum Nutr,2009,64:86−93. doi: 10.1007/s11130-009-0108-1
[6]	Tong L T, Guo L N, Zhou X R, et al. Effects of dietary oat proteins on cholesterol metabolism of hypercholesterolaemic hamsters[J]. J Sci Food Agric,2016,96:1396−1401. doi: 10.1002/jsfa.7236
[7]	Motoni K, Masatoshi K, Reiko W. Physiological multifunctions of rice proteins of endosperm and bran[J]. J Nutr SCI Vitaminol,2019,65:S42−S47. doi: 10.3177/jnsv.65.S42
[8]	Ladda W, Chockchai T, Samanthi W, et al. Isolation and identification of antioxidant peptides from enzymatically hydrolyzed rice bran protein[J]. Food Chemistry,2016,192:156−162. doi: 10.1016/j.foodchem.2015.06.057
[9]	Zhang H J, Glenn E B, Cheryl R M, et al. Lower weight gain and hepatic lipid content in hamsters fed high fat diets supplemented with white rice protein, brown rice protein, soy protein, and their hydrolysates[J]. J Agric Food Chem,2011,59:10927−10933. doi: 10.1021/jf202721z
[10]	Cai J X, Yang L, Hong J H, et al. Antioxidant capacity responsible for a hypocholesterolemia is independent of dietary cholesterol in adult rats fed rice protein[J]. Gene,2014,533(1):57−66. doi: 10.1016/j.gene.2013.09.124
[11]	Mustapha U I, Aminu I, Der J O, et al. Germinated brown rice regulates hepatic cholesterol metabolism and cardiovascular disease risk in hypercholesterolaemic rats[J]. Journal of Functional Foods,2014,8:193−203. doi: 10.1016/j.jff.2014.03.013
[12]	Folch J, Lees M, Sloane Stanley G H. A simple method for the isolation and purification of total lipids from animal tissues[J]. J Biol Chem,1957,226:497−509. doi: 10.1016/S0021-9258(18)64849-5
[13]	Bruusgaard A, Hanna Sørensen, C C Gilhuus-Moe, et al. Bile acid determination with different preparations of 3α-hydroxysteroid dehydrogenase[J]. Clin Chim Acta,1977,77:387−395. doi: 10.1016/0009-8981(77)90245-5
[14]	Malchow-Møller A, Arffmann S, Larusso N F, et al. Enzymatic determination of total 3α-hydroxy bile acids in feces validationin healthy subjects of a rapid method suitable for clinical routine purpose[J]. Scand J Gastroenterol,1982,17:331−333. doi: 10.3109/00365528209182063
[15]	Satoshi N, Atsushi N, Haruhiko S, et al. Soystatin (VAWWMY), decreased micellar solubility and inhibited cholesterol absorption in rats[J]. Biosci Biotechnol Biochem, 2010, 74: 1738−1741.
[16]	Zhang H J, Wang J, Liu Y L, et al. Rice bran proteins and their hydrolysates modulate cholesterol metabolism in mice on hypercholesterolemic diets[J]. Food Funct,2016,7:2747−2753. doi: 10.1039/C6FO00044D
[17]	Varun K, Lakshmy V K, Purnima K T. The modulatory effect of cholesterol synthesis by oryza sativa derived bioactive peptide fractions: An in vitro investigation[J]. International Journal of Peptide Research and Therapeutics,2021,27:245−251. doi: 10.1007/s10989-020-10079-7
[18]	邵素娟, 丁方莉, 刘迪迪, 等. 食物蛋白源降血脂肽的研究进展[J]. 食品工业科技,2018(1):323−326. [Shao Sujuan, Ding Fangli, Liu Didi, et al. Research progress of food protein derived lipid-lowering peptides[J]. Science and Technology of Food Industry,2018(1):323−326.
[19]	Spady D K, Cuthbert J A, Willard M N. Overexpression of cholesterol 7α-hydroxylase(CY7A) in mice lacking the low density lipoprotein (LDL) receptor gene[J]. Biol Chem,1998,273:126−132. doi: 10.1074/jbc.273.1.126
[20]	包小兰, 刘晓静, 郑睿, 等. 亚麻籽降胆固醇活性肽的酶解工艺优化及分级制备[J]. 中国油脂,2020,45(6):30−35. [Bao Xiaolan, Liu Xiaojing, Zheng Rui, et al. Optimization of enzymatic hydrolysis process and fractionated preparation of cholesterol-lowering active peptides from flax seed[J]. China Oils and Fat,2020,45(6):30−35. doi: 10.12166/j.zgyz.1003-7969/2020.06.006
[21]	Zhang H J, Wallace H, Yokoyama, et al. Concentration-dependent displacement of cholesterol in micelles by hydrophobic rice bran protein hydrolysates[J]. J Sci Food Agric,2012,92:1395−1401. doi: 10.1002/jsfa.4713
[22]	Anderson James W, Johnstone Bryan M, Cook Newell Margaret E. Meta-analysis of the effect of soy protein intake on serum lipids[J]. N Engl J Med,1995,333:276−282. doi: 10.1056/NEJM199508033330502
[23]	Miki S, Tomo K, Marina N J, et al. Effects of dietary intake of soy protein and isoflavones on cardiovascular disease risk factors in high risk, middle-aged men in Scotland[J]. J Am Coll Nutr,2004,23:85−91. doi: 10.1080/07315724.2004.10719347
[24]	Elisa R, Consuelo S M, E Miramontes, et al. Nutraceutical composition, antioxidant activity and hypocholesterolemic effect of a water-soluble enzymatic extract from rice bran[J]. Food Res Int,2009,42:387−393. doi: 10.1016/j.foodres.2009.01.010
[25]	于美汇, 赵鑫, 尹红力, 等. 碱提醇沉黑木耳多糖体外和体内降血脂功能[J]. 食品科学,2017,38(1):232−237. [Yu Meihui, Zhao Xin, Yin Hongli, et al. Effects of alkali extraction and alcohol precipitation polysaccharides from Auricularia auriculata on lipids lowering in vitro and in vivo[J]. Food Science,2017,38(1):232−237. doi: 10.7506/spkx1002-6630-201701039
[26]	朱维, 邹波, 葛珍珍, 等. 柿单宁的体外降胆固醇作用[J]. 食品科学,2016,37(9):71−76. [Zhu Wei, Zou Bo, Ge Zhenzhen, et al. Cholesterol lowering effect of persimmon tannin in vitro[J]. Food Science,2016,37(9):71−76. doi: 10.7506/spkx1002-6630-201609014

Supplements (0)

Cited By

Cited by

Periodical cited type(2)

1.	宋丽丽，霍姗浩，胡冉冉，赵鑫淼，朱钰琪，杨旭，张志平，魏涛. 复合乳酸菌固态发酵对脱脂米糠理化性质、生物活性和功能特性的影响. 轻工学报. 2024(03): 21-28 .
2.	李雨欣，胡芸利，刘聪，边瑞琴，包小兰，王吉力特. 河套麦胚多肽的制备工艺优化及其体外降血脂活性. 食品工业科技. 2024(17): 174-180 . 本站查看