CHEN Shuping, LI Mingzhi, DONG Nan, et al. Immunomodulatory Effects of Different Edible Fungi Polysaccharide Complex on RAW264.7 Macrophages[J]. Science and Technology of Food Industry, 2021, 42(17): 366−372. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110143.
Citation: CHEN Shuping, LI Mingzhi, DONG Nan, et al. Immunomodulatory Effects of Different Edible Fungi Polysaccharide Complex on RAW264.7 Macrophages[J]. Science and Technology of Food Industry, 2021, 42(17): 366−372. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110143.

Immunomodulatory Effects of Different Edible Fungi Polysaccharide Complex on RAW264.7 Macrophages

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  • Received Date: November 15, 2020
  • Available Online: July 05, 2021
  • Objective: The present study was carried on to assess the immunoregulatory effects of complexes obtained by any 3 polysaccharides from five kinds of different edible fungi polysaccharides, including Tremella fuciformis polysaccharide, Poriacocos polysaccharide, Lentinan, Hericium edodes polysaccharide and Dictyophora indusiata polysaccharide. Methods: Effects of different concentrations of complexes on the proliferation of RAW264.7 were explored using cell counting kit (CCK)-8, the levels of tumor necrosis factor (TNF)-α in the culture medium were detected after complexes treatment by enzyme-linked immunosorbent assay (ELISA). In addition, flow cytometry was employed to analyze the effect of complexes on RAW 264.7 phagocytic capacity. Results: Compared with normal control group, ten kinds of complexes exerted better positive effects on the proliferation of macrophages. Meanwhile, the complexes obviously enhanced phagocytic capacity as well as increased the TNF-α level. Furthermore, 10 µg/mL complex of Tremella fuciformis polysaccharide, Poriacocos polysaccharide and Dictyophora indusiata polysaccharide exhibited the best effect on the proliferation of macrophages, 160 µg/mL complex of Tremella fuciformis polysaccharide, Poriacocos polysaccharide and Lentinan showed the best effect on enhancing both secretion of TNF-α and phagocytic ability of RAW264.7. Conclusion: The complexes of polysaccharides from these five kinds of edible fungi displayed excellent effect on regulating immunity of macrophage RAW 264.7. The results could provide data reference for the development of immunomodulatory polysaccharide products.
  • [1]
    Brodin Petter, Davis Mark M. Human immune system variation[J]. Nature Reviews Immunology,2017,17(1):21−29. doi: 10.1038/nri.2016.125
    [2]
    Backhed Fredrik, Ley Ruth E, Sonnenburg Judtin L, et al. Host-bacterial mutualism in the human intestine[J]. Science,2005,307(5717):1915−1920. doi: 10.1126/science.1104816
    [3]
    袁志鹰, 资源, 谢梦洲. 中医药膳食疗对抑郁症的防治作用研究进展[J]. 中国中医药现代远程教育,2020,18(16):146−149. [Yuan Z Y, Zi Y, Xie M Z. Research progress on traditional Chinese medicine diet medication therapy in the prevention of depression[J]. Modern Distance Education of Chinese Traditional Medicine,2020,18(16):146−149. doi: 10.3969/j.issn.1672-2779.2020.16.060
    [4]
    陈萌山. 会吃才有健康[J]. 农产品市场,2020(6):8−21. [Chen M S. Smart eating to be healthy[J]. Markets of Agricultural Products,2020(6):8−21.
    [5]
    Bacher P, Kniemeyer O, Schonbrunn A, et al. Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi[J]. Mucosal Immunology,2014,7(4):916−928. doi: 10.1038/mi.2013.107
    [6]
    黄青, 李丽媛, 刘晴晴, 等. 灵芝多糖和猪苓多糖及其复方的免疫调节作用研究进展[J]. 食品科学,2020,41(17):275−282. [Huang Q, Li L Y, Liu Q Q, et al. Advances in immunoregulation effects of Ganoderma lucidum polysaccharide and/or Polyporus umbellatus polysaccharide[J]. Food Science,2020,41(17):275−282. doi: 10.7506/spkx1002-6630-20190813-149
    [7]
    李依霖, 姜明, 谭明琪. 食用菌多糖的药用价值研究进展[J]. 宁夏农林科技,2020,61(5):24−26, 39. [Li Y L, Jiang M, Tan M Q. Research progress on pharmacological value of polysaccharides from edible fungi[J]. Ningxia Journal of Agriculture and Forestry Science and Technology,2020,61(5):24−26, 39. doi: 10.3969/j.issn.1002-204x.2020.05.009
    [8]
    Zhou Y L, Chen X Y, Yi R K, et al. Immunomodulatory effect of Tremella polysaccharides against cyclophosphamide-induced immunosuppression in mice[J]. Molecules,2018,23(2):239. doi: 10.3390/molecules23020239
    [9]
    Luo X, Huang S W, Luo S, et al. Identification of genes underlying the enhancement of immunity by a formula of lentinan, pachymaran and tremelia polysaccharides in immunosuppressive mice[J]. Scientific Reports,2018,8:10082. doi: 10.1038/s41598-018-28414-w
    [10]
    Zhang Q, Cong R H, Hu M H, et al. Immunoenhancement of edible fungal polysaccharides (lentinan, tremellan, and pachymaran) on cyclophosphamide-induced immunosuppression in mouse model[J]. Evidence-Based Complementary and Alternative Medicine,2017,2017:9459156.
    [11]
    Chen S P, Liu C C, Huang X J, et al. Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts[J]. Journal of Functional Foods,2020,66:103791. doi: 10.1016/j.jff.2020.103791
    [12]
    Wu F F, Zhou C H, Zhou D D, et al. Structure characterization of a novel polysaccharide from Hericiumerinaceus fruiting bodies and its immunomodulatory activities[J]. Food & Function,2018,9(1):294−306.
    [13]
    尚京迎, 付海田, 邓超, 等. 基于巨噬细胞模型的竹荪多糖的免疫功能[J]. 食品与生物技术学报,2016,35(8):849−854. [Shang J Y, Fu H T, Deng C, et al. Immunomodulatory effects of a polysaccharide from Dictyophora indusiata om macrophage[J]. Journal of Food Science and Biotechnology,2016,35(8):849−854. doi: 10.3969/j.issn.1673-1689.2016.08.010
    [14]
    赵明明. 黑灵芝多糖对小鼠肠道黏膜免疫及黏膜损伤的影响[D]. 南昌: 南昌大学, 2018.

    Zhao M M. Effect of polysaccharide from Ganoderma atrum on intestinal mucosal immunity and mucosal injury in mice[D]. Nanchang: Nanchang University, 2018.
    [15]
    Yu Q, Nie S P, Wang J Q, et al. Signaling pathway involved in the immunomodulatory effect of Ganoderma atrumpolysaccharide in spleen lymphocytes[J]. Journal of Agricultural and Food Chemistry,2015,63(10):2734−2740. doi: 10.1021/acs.jafc.5b00028
    [16]
    Sun S H, Li K J, Xiao L, et al. Characterization of polysaccharide from Helicteres angustifolia L. and its immunomodulatory activities on macrophages RAW264.7[J]. Biomedicine & Pharmacotherapy,2019,109:262−270.
    [17]
    Ubanako Philemon, Xelwa Ntombikayise, Ntwasa Monde. LPS induces inflammatory chemokines via TLR-4 signalling and enhances the Warburg effect in THP-1 cells[J]. Plos One,2019,14(9):e0222614. doi: 10.1371/journal.pone.0222614
    [18]
    童微, 余强, 李虎, 等. 铁皮石斛多糖化学修饰及其对免疫活性的影响[J]. 食品科学,2017,38(7):155−160. [Tong W, Yu Q, Li H, et al. Chemial modification and immunoregulatory activity of polysaccharides from Dendrobium officinale[J]. Food Science,2017,38(7):155−160. doi: 10.7506/spkx1002-6630-201707025
    [19]
    Wang J Q, Nie S H P, Cui Steve W, et al. Structural characterization and immunostimulatory activity of a glucan from natural Cordyceps sinensis[J]. Food Hydrocolloids,2017,67:139−147. doi: 10.1016/j.foodhyd.2017.01.010
    [20]
    Wu J J, Xu Y B, Zhu B, et al. Characterization of an arabinogalactan from the fruit hulls of Ficus pumila Linn. and its immunomodulatory effect[J]. Journal of Functional Foods,2020,73:104091. doi: 10.1016/j.jff.2020.104091
    [21]
    王萌皓, 郝正祺, 常明昌, 等. 广叶绣球菌β-D-葡聚糖对巨噬细胞RAW264.7免疫调节作用受体TLR4和TLR2的影响[J]. 菌物学报,2020,39(5):907−916. [Wang M H, Hao Z Q, Chang M C, et al. Effects of Sparassis latifolia β-D-glucan on immune receptors TLR4 and TLR2 of RAW254.7 macrophages[J]. Journal of Fungus,2020,39(5):907−916.
    [22]
    陈炅然, 胡庭俊, 程富胜, 等. 蕨麻多糖对小鼠淋巴细胞增殖和一氧化氮分泌的影响[J]. 中国兽医科学,2005,35(9):735−738. [Chen J R, Hu T J, Cheng F S, et al. Effect of Potentilla anserine polysaccharide on proliferation of splenic lymphocytes and production of nitric oxide in mice[J]. Veterinary Science in China,2005,35(9):735−738.
    [23]
    周妍, 王凌, 孙利芹, 等. 5种海洋微藻多糖体外免疫调节活性的筛选[J]. 海洋通报,2010(2):76−80. [Zhou Y, Wang L, Sun L Q, et al. Immunomodulation activities of polysaccharides in vitro from five micro-algae[J]. Marine Science Bulletin,2010(2):76−80.
    [24]
    张凯月, 李春楠, 兰梦, 等. 鹿胎肽对巨噬细胞RAW264.7的免疫调节作用[J]. 食品工业科技,2021,42(1):342−347. [Zhang K Y, Li C N, Lan M, et al. Immunomodulatory effects of peptides from deer fetus on RAW264.7 macrophages[J]. Science and Technology of Food Industry,2021,42(1):342−347.
    [25]
    Paterson R R M. Ganoderma—A therapeutic fungal biofactory[J]. Phytochemistry,2006,67(18):1985−2001. doi: 10.1016/j.phytochem.2006.07.004
    [26]
    Cunther C, Martini E, Wittkopf N, et al. Caspase-8 regulate TNF-α-induced epithelial and terminal ileitis[J]. Nature,2011,477(7364):335−339. doi: 10.1038/nature10400
    [27]
    刘苏, 姜玥, 罗建平, 等. 5种食用菌多糖理化性质及免疫活性的比较研究[J]. 食品科学,2015,36(13):252−256. [Liu S, Jiang Y, Luo J P, et al. Physicochemical properties and immunomodulating activities of polysaccharides from five species of edible mushrooms[J]. Food Science,2015,36(13):252−256. doi: 10.7506/spkx1002-6630-201513047
    [28]
    Miller Andrew M, Horiguchi Norio, Jeong Won-II, et al. Molecular mechanisms of alcoholic liver disease: innate immunity and cytokines[J]. Alcoholism-Clinical and Experimental Research,2011,35(5):787−793. doi: 10.1111/j.1530-0277.2010.01399.x
    [29]
    吴珊, 罗刚健, 姚伟锋, 等. 右美托咪定受体依赖性增强巨噬细胞吞噬功能[J]. 中山大学学报(医学科学版),2020,41(2):268−272. [Wu S, Luo G J, Yao W F, et al. Dexmedetomidine receptor dependently enhanced macrophage phagocytosis[J]. Journal of Sun Yat-sen University (Medical Sciences),2020,41(2):268−272.
    [30]
    王淑惠, 杨玉洁, 周爱梅, 等. 两种方法提取佛手渣多糖及其对巨噬细胞RAW264.7免疫调节活性的研究[J]. 食品工业科技,2020,41(15):179−187. [Wang S H, Yang Y J, Zhou A M, et al. Study on the polysaccharide extracted from Bergamot (Citrus medica L. var. sarcodactylis) residue by two methods and its immunomodulatory function in RAW264.7 cells[J]. Science and Technology of Food Industry,2020,41(15):179−187.
    [31]
    Yu Q, Nie S P, Li W J, et al. Macrophage immunomodulatory activity of a purified polysaccharide isolated from Ganoderma atrum[J]. Phytotherapy Research,2013,27(2):186−191. doi: 10.1002/ptr.4698
    [32]
    朱淼. 喇叭菌多糖(CC-P)和竹荪多糖(DI-P)结构、生物活性比较研究及其相关分子机制初探[D]. 南充: 西华师范大学, 2020.

    Zhu M. Comparative study on structure and bioactivities of Cantharellus cinnabarinus polysaccaride and Dictyophora polysaccharideand preliminary exploration of related molecular mechanisms[D]. Nanchong: China West Normal University, 2020.
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