Protectivue of <i>Epimedium</i> Alkaloid on Reproductive System Injury Induced by Cyclophosphamide in Male Mice

DING Yue; XU Xiaoyu; WANG Yafei; GAO Yanlei; SHEN Minghao

doi:10.13386/j.issn1002-0306.2020110125

Volume 42 Issue 21

Oct. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(21): 353-360. > DOI: 10.13386/j.issn1002-0306.2020110125

DING Yue, XU Xiaoyu, WANG Yafei, et al. Protectivue of Epimedium Alkaloid on Reproductive System Injury Induced by Cyclophosphamide in Male Mice[J]. Science and Technology of Food Industry, 2021, 42(21): 353−360. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110125.

Citation:

PDF (2385 KB)

Protectivue of Epimedium Alkaloid on Reproductive System Injury Induced by Cyclophosphamide in Male Mice

1.
College of Food Science and Technology, Jilin Agricultural University, Changchun 130000, China
2.
SGS-CSTG, Dalian 116601, China

More Information

Received Date: November 12, 2020
Available Online: September 05, 2021

Graphical Abstract

Abstract

Abstract

Objective:To study the protective mechanism of Epimedium alkaloids against cyclophosphamide induced reproductive injury in male mice. Methods: First, 40 male mice of Kunming species were randomly divided into blank group and Epimedium low, medium and high dose groups to investigate whether Epimedium alkaloid had reproductive toxicity to male mice. Secondly, another 40 Kunming male mice were injected with cyclophosphamide for modeling, and randomly divided into cyclophosphamide group, cyclophosphamide-alkaloid low, medium and high dose groups. After successful modeling, the mice in each dose group were given the drug for 30 days by gavage except the cyclophosphamide group. After the last administration, mice were sacrificed and the quality of epididymis, testis and sperm of different groups of mice were measured. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), testosterone and Bcl-2/Bax protein in testicular tissue were detected. Results: Epimedium alkaloid had no reproductive toxicity on male mice. Spermatozoa quality and testicular activity decreased significantly after injection of cyclophosphamide, and testicular tissue of mice was seriously damaged, which proved the success of modeling. Compared with the cyclophosphamide group, epididymal coefficient and testicular coefficient of mice in each dose group were significantly increased, sperm number increased, sperm viability increased, sperm malformation rate decreased, SOD activity in testicular tissue increased, MDA content decreased, testosterone level increased, and Bcl-2 /Bax protein ratio increased. Through the observation of testicular tissue section, the damage of testicular tissue was reduced. Conclusion: Epimedium alkaloid can protect the reproductive system of male mice to some extent.
- Epimedium,
- alkaloids,
- cyclophosphamide,
- reproductive system,
- sperm quality

FullText(HTML)

References (28)

References

[1]	高林. 海参多糖对环磷酰胺致小鼠生殖系统受损拮抗作用的实验研究[D]. 青岛: 青岛大学, 2014. Gao L. Experimental study on antagonistic effect of sea cucumber polysaccharide on reproductive system damage induced by cyclophosphamide in mice [D]. Qingdao: Qingdao University, 2014.
[2]	张龙. 离子交换法用于淫羊藿活性成分综合利用工艺的研究[D]. 青岛: 青岛科技大学, 2012. Zhang L. Comprehensive utilization of active components in Epimedium by ion exchange method[D]. Qingdao: Qingdao University, 2012.
[3]	熊志晓, 陈雪娇, 徐长远, 等. 纳米氧化锌对雄性小鼠生殖系统的影响[J]. 农业开发与装备,2019(5):124, 113. [Xiong Z X, Chen X J, Xu C Y, et al. Effects of nano zinc oxide on reproductive system of male mice[J]. Agricultural Development and Equipment,2019(5):124, 113.
[4]	吴雪韦. 朝鲜淫羊藿的化学成分及生物活性研究[D]. 济南: 山东大学, 2018. Wu X W. Studies on chemical constituents and biological activities of Epimedium Korean[D]. Jinan: Shandong University, 2018.
[5]	Zheng Hong, He Bing, Wu Tianxiu, et al. Extraction, purification and anti-osteoporotic activity of a polysaccharide from Epimedium brevicornum Maxim. in vitro[J]. International Journal of Biological Macromolecules,2020:156.
[6]	李聪聪, 赵鹏, 秦燕勤, 等. 淫羊藿苷的药理活性研究进展[J]. 中医学报,2020,35(4):781−78. [Li C C, Zhao P, Qin Y Q, et al. Advances in pharmacological activities of icariin[J]. Chinese Journal of Traditional Chinese Medicine,2020,35(4):781−78.
[7]	韩松辰. 淫羊藿素对食管癌干细胞生理活性的影响及机制研究[D]. 兰州: 甘肃中医药大学, 2019. Han S C. Effect of icariin on physiological activity of esophageal cancer stem cells and its mechanism[D]. Lanzhou: Gansu University of Traditional Chinese Medicine, 2019.
[8]	刘春明, 刘志强, 窦建鹏, 等. 朝鲜淫羊藿中生物碱类新成分的分离提取及结构鉴定[J]. 高等学校化学学报,2003(12):2215−2217. [Liu C M, Liu Z Q, Dou J, et al. Isolation, extraction and structural identification of new alkaloids from Epimedium[J]. Chemical Journal of Chinese Universities,2003(12):2215−2217. doi: 10.3321/j.issn:0251-0790.2003.12.035
[9]	Yao Yu, Liu Xiang, Yang Qianru, et al. The complete chloroplast genome of Epimedium brevicornu (Berberidaceae), a traditional Chinese medicinal herb[J]. Mitochondrial DNA Part B,2020,5(1):887−888. doi: 10.1080/23802359.2020.1718027
[10]	杨席伟, 王佳, 杨毅坚. 淫羊藿主要活性成分在泌尿生殖系统应用研究进展[J]. 云南中医中药杂志,2020,41(3):92−96. [Yang Q W, Wang J, Yang Y J. Research progress in the application of main active ingredients in Epimedium in genitourinary system[J]. Journal of Yunnan Traditional Chinese Medicine and Traditional Chinese Medicine,2020,41(3):92−96. doi: 10.3969/j.issn.1007-2349.2020.03.028
[11]	Seo Chang Seob, Kim Jung Hoon, Lee Sullim, et al. Physical and chemical analysis of Epimedii herba by the packaging methods and storage periods[J]. Korean Journal of Pharmacognosy,2019,50(4):312−317.
[12]	Dieter Slos, Vladimir V Yushin, Myriam Claeys, et al. Structure, development, and evolutive patterns of spermatozoa in rhabditid nematodes (Nematoda: Rhabditida)[J]. Journal of Morphology,2020,281(11):1411−1435.
[13]	韦贤, 彭兴, 王金妮, 等. 老鼠簕生物碱A对环磷酰胺致小鼠生殖系统损伤的保护作用[J]. 右江民族医学院学报,2018,40(2):103−107. [Wei X, Peng X, Wang J N, et al. Protective effect of Acanthus acanthus alkaloid A on reproductive system injury induced by cyclophosphamide in mice[J]. Journal of Youjiang Medical College for Nationalities,2018,40(2):103−107. doi: 10.3969/j.issn.1001-5817.2018.02.002
[14]	Huang Xiaowen, Wang Xuping, Zhang Yang, et al. Absorption and utilisation of epimedin C and icariin from Epimedii herba, and the regulatory mechanism via the BMP2/ Runx2 signalling pathway[J]. Biomedicine & Pharmacotherapy,2019,54(1):28−35.
[15]	孙潇翔, 向娥, 邱帅凯, 等. 吡咯里西啶生物碱毒性作用研究进展[J]. 中国药物戒,2019,16:76−80. [Sun X X, Xiang E, Qiu S K, et al. Progress in toxicity of pyrrolizidine alkaloids[J]. Chinese Journal of Medicine,2019,16:76−80.
[16]	Li Chong, Liao Lanqing, Sun Linjun, et al. Icariin stimulates hFOB 1.19 osteoblast proliferation and differentiation via OPG/RANKL mediated by the estrogen receptor[J]. Current Pharmaceutical Biotechnology,2021,22(1).
[17]	Xiao fang, Wang, Jun, et al. High-throughput metabolomics method for discovering metabolic biomarkers and pathways to reveal effects and molecular mechanism of ethanol extract from epimedium against osteoporosis[J]. Frontiers in pharmacology, 2020, 11: 145.
[18]	芦春斌, 张裕明, 朱倍倍, 等. 铁皮石斛多糖对顺铂引起的雄鼠生殖系统损伤的影响[J]. 中国药学杂志,2018,54(1):28−35. [Lu C B, Zhang Y M, Zhu B B, et al. Effects of Dendrobium officinale polysaccharide on male rat reproductive system injury induced by cisplatin[J]. Chin J Pharmacol,2018,54(1):28−35.
[19]	刘倩, 田元春, 宾彬, 等. 菟丝子黄酮对生殖系统的保护作用研究进展[J]. 中医药导报,2018,26(12):148−151, 156. [Liu Q, Tian Y C, Bin B, et al. Research progress on protective effect of dodder flavonoids on reproductive system[J]. Chinese Journal of Traditional Chinese Medicine,2018,26(12):148−151, 156.
[20]	刘建国, 赵红乐, 赵宇涛, 等. 补肾活血方对环磷酰胺所致小鼠睾丸氧化应激损伤及生精损伤的影响[J]. 中国中医药信息杂志,2020,27(8):68−72. [Liu J G, Zhao H L, Zhao Y T, et al. Effect of Bushen Huoxue prescription on oxidative stress and spermatogenesis injury in mice induced by cyclophosphamide[J]. Chinese Journal of Traditional Chinese Medicine Information,2020,27(8):68−72.
[21]	潘晓燕, 李康, 王喜艳, 等. 温肾生精饮对环磷酰胺致小鼠睾丸氧化损伤的保护作用[J]. 军事医学,2014,38(12):957−961. [Pan X Y, Li K, Wang X Y, et al. Protective effect of wenshen shengjing Yin on oxidative damage of testis induced by cyclophosphamide in mice[J]. Military Medicine,2014,38(12):957−961.
[22]	Liu Hao, Hu Haibo, Zhang Shuihan, et al. The complete chloroplast genome of the rare species Epimedium tianmenshanensis[J]. Molecular Biology of Plants,2020,26(prepublish):186.
[23]	杨翠军, 孙全文, 葛剑, 等. 马齿苋多糖抗雄性大鼠生殖氧化损伤作用的研究[J]. 现代食品科技,2014,30(2):6−11. [Yang C W, Sun Q W, Ge J E, et al. Effects of Portulaca oleracea polysaccharides on reproductive oxidative damage in male rats[J]. Modern Food Science and Technology,2014,30(2):6−11.
[24]	Li Ning, Xie Lijuan, Yang Na, et al. Rapid classification and identification of chemical constituents in Epimedium koreanum Nakai by UPLC-Q-TOF-MS combined with data post-processing techniques[J]. Phytochemical Analysis: PCA,2020,32(2):79.
[25]	张晓伟, 金瑛, 易东旭, 等. 十一酸睾酮原位凝胶植入剂避孕效果及对雄性大鼠生殖系统的影响[J]. 医药导报,2020,39(11):1496−1501. [Zhang X W, Jin Y, Yi D X, et al. Effects of testosterone undecanoate in situ gel implant on contraceptive effect and reproductive system of male rats[J]. J Med Review,2020,39(11):1496−1501.
[26]	Suo Fengmei, Liu Xiang, Zhang Cheng, et al. The complete chloroplast genome of Epimedium elachyphyllum Stearn (Berberidaceae), an endangered species endemic to China[J]. Mitochondrial DNA Part B,2020,5(1):23−27. doi: 10.1080/23802359.2019.1694849
[27]	杨飞来, 罗杰, 邓敦, 等. 淫羊藿提取物和止痢草油对公猪精液量和精液品质的影响[J]. 湖南饲料,2020(4):39−41. [Yang F L, Luo J, Deng D, et al. Effects of Epimedium extract and pullorum oil on semen volume and quality of Boar[J]. Hunan Feed,2020(4):39−41. doi: 10.3969/j.issn.1673-7539.2020.04.014
[28]	李环, 曲振廷, 钱鸿昊, 等. 人参皂苷Rg1联合人参皂苷Rg3对雄性生殖功能损伤模型小鼠生殖功能的改善作用[J]. 吉林大学学报(医学版),2020,46(4):707−713. [Li H, Qu Z Y, Qian H H, et al. Effects of ginsenoside Rg1 combined with ginsenoside Rg3 on reproductive function in male reproductive function injury model mice[J]. Journal of Jilin University (Medical Science Edition),2020,46(4):707−713.

Supplements (0)

Cited By

Cited by

Periodical cited type(9)

1.	刘学艳，吴成远，徐婷，罗正飞，刘艳红，王绍梅. 不同品种工夫红茶挥发性物质研究. 中国茶叶. 2025(04): 62-67 .
2.	徐旭华，黄文洁，陈旭峰，陈园园，吴绍文，李红建，晏石娟. ‘丹霞2号’红茶加工过程中品质特征成分的动态变化研究. 园艺学报. 2024(01): 145-161 .
3.	孙宏，姚晓红，吴逸飞，王新，沈琦，汤江武. 粉绿狐尾藻固态发酵饲料的营养成分及其代谢物分析. 中国畜牧杂志. 2023(04): 186-192 .
4.	唐佳代，冉光耀，陈诺，王芙蓉，赵腾飞，冷枝，王怡，王士超. 基于非靶向代谢组学分析不同陈化时间老鹰茶代谢产物的差异. 中国酿造. 2023(09): 115-119 .
5.	杨云飞，王玉婉，林家正，涂政，蓝天梦，陈琳，叶阳. 发酵中氧对红茶品质的影响及富氧发酵工艺优化. 食品工业科技. 2023(19): 199-207 . 本站查看
6.	夏长杙，冉乾松，蒲璐璐，刘亚兵. 不同发酵方式对古茶树鲜叶制备工夫红茶品质的影响. 中国酿造. 2023(10): 127-135 .
7.	陈霞，唐晨阳，马昕怡，邵童，李晓兵，顾瑞霞. 超微茶粉对益生菌慕斯品质及抗氧化活性的影响. 食品与发酵工业. 2022(10): 194-198 .
8.	刘亚芹，王辉，杨霁虹，周汉琛，黄建琴，雷攀登. 基于滋味成分变化的祁门红茶发酵程度差异研究. 茶叶通讯. 2022(02): 193-201 .
9.	曾议霆，吴雪莉，杨春梅，刘高杰，高丙德，唐克纯. 基于非靶向代谢组学比较不同发酵方式红茶滋味物质差异. 食品安全质量检测学报. 2022(16): 5288-5296 .