Optimization of Fermentation Conditions and Enzymatic Characteristics of the Cholesterol Esterase from <i>Pichia pastoris</i>

QUAN Haoyan; WANG Peng; WEI Zhengpeng; DU Chunying; SHEN Zhaopeng; ZHANG Jingliang; QIAO Leke

doi:10.13386/j.issn1002-0306.2020110102

Volume 42 Issue 17

Aug. 2021

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Science and Technology of Food Industry > 2021 > 42(17): 94-99. > DOI: 10.13386/j.issn1002-0306.2020110102

QUAN Haoyan, WANG Peng, WEI Zhengpeng, et al. Optimization of Fermentation Conditions and Enzymatic Characteristics of the Cholesterol Esterase from Pichia pastoris [J]. Science and Technology of Food Industry, 2021, 42(17): 94−99. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110102.

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Optimization of Fermentation Conditions and Enzymatic Characteristics of the Cholesterol Esterase from Pichia pastoris

1.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
2.
Qingdao Municipal Hospital, Qingdao 266011, China
3.
National and Local United Engineering Lab of Marine Functional Food, Rongcheng 264309, China
4.
Marine Biomedical Research Institute of Qingdao, Qingdao 266071, China

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Received Date: November 10, 2020
Available Online: July 03, 2021

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Abstract

Abstract

In order to achieve the high-efficiency fermentation of cholesterol esterase, Pichia pastoris was selected as the research object, and its fermentation conditions were optimized by single factor and orthogonal experiments. The results showed that the optimum medium was yeast extract 1.0%, peptone 1.5%, glycerin 1.0%, KH₂PO₄ 1.18%, K₂HPO₄ 0.3%, biotin 4×10^-5%, YNB 1.34%. Under the condition of initial pH7.0, fermentation temperature 30 ℃, fermentation time 96 h, the enzyme activity could reach 11.21 U/mL. And it was 3.65 times higher than before. The results of enzymatic properties showed that the optimum pH and reaction temperature of the enzyme were 8.0 and 50 ℃, respectively. Zn²⁺ had a strong inhibition on the enzyme. The cholesterol esterase had stable enzymatic properties and could provide technical basis for large-scale production in the future.
- Pichia pastoris,
- cholesterol esterase,
- fermentation conditions,
- orthogonal optimization,
- characterization

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