Effect of Dry Methods on Differential Protein of Peony Peta Based on the Analysis of Label-Free Quantitative Proteomics

Ning XU; Yuehan ZHAO; Zhaohua HOU

doi:10.13386/j.issn1002-0306.2020110094

Volume 42 Issue 9

Apr. 2021

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Science and Technology of Food Industry > 2021 > 42(9): 136-144. > DOI: 10.13386/j.issn1002-0306.2020110094

XU Ning, ZHAO Yuehan, HOU Zhaohua. Effect of Dry Methods on Differential Protein of Peony Peta Based on the Analysis of Label-Free Quantitative Proteomics [J]. Science and Technology of Food Industry, 2021, 42(9): 136−144. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110094.

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Effect of Dry Methods on Differential Protein of Peony Peta Based on the Analysis of Label-Free Quantitative Proteomics

Ning XU^1,,
Yuehan ZHAO²,
Zhaohua HOU^{1, 2, ,}

1.
Shandong Academy of Agricultural Machinery Sciences, Jinan 250000, China
2.
College of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China

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Received Date: November 10, 2020
Available Online: March 15, 2021

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Abstract

Abstract

In order to explore the protein differences of different processing methods at the protein level on peony petal(hot air drying, HD; natural drying, ZD; vacuum freeze drying, FD), and provide theoretical basis for subsequent processing, the proteomic differences of three kinds of processed peony petal were compared by label-free proteomics. The results showed that: A total of 1423 proteins were identified and 1349 proteins (94.80%)were quantified in the three groups. 1325, 677, and 1085 proteins were quantified in FD, HD and ZD respectively. There were 656 proteins in the three groups. Compared with HD, 77 differentially expressed proteins (DEPs) were detected in ZD, up-regulated 24 proteins, down-regulated 53 proteins. 77 DEPs annotation using GO and KEGG database revealed that proteins were enriched in glycolysis/gluconeogenesis pathway, pyruvate metabolism, TCA cycle, protein processing in endoplasmic reticulum. Conclusion: Processing methods had significant effect on protein difference of peony. This study is the first time to analyze the expression changes of differential proteins in peony flower by proteomics method, and provide scientific basis for different processing methods in practice.
- peony petal,
- vacuum freeze drying,
- hot air drying,
- natural drying,
- label-free quantification (LFQ),
- proteomics

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