PMP-HPLC Fingerprint of Partial Acid Hydrolysate of <i>Lycium barbarum</i> Polysaccharides Based on the Chemometric Methods

XI Jingrui; ZHANG Yilin; WU Mengqi; ZHANG Wenqing; XU Zhizhen; XIA Wei

doi:10.13386/j.issn1002-0306.2020110002

Volume 42 Issue 18

Sep. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(18): 268-275. > DOI: 10.13386/j.issn1002-0306.2020110002

XI Jingrui, ZHANG Yilin, WU Mengqi, et al. PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Lycium barbarum Polysaccharides Based on the Chemometric Methods[J]. Science and Technology of Food Industry, 2021, 42(18): 268−275. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110002.

Citation:

PDF (1427 KB)

PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Lycium barbarum Polysaccharides Based on the Chemometric Methods

East China University of Science and Technology, Shanghai Key Laboratory of Functional Materials Chemistry, Shanghai 200237, China

More Information

Received Date: November 02, 2020
Available Online: July 19, 2021

Graphical Abstract

Abstract

Abstract

In this paper, the fingerprints of partial acid hydrolysates of Lycium barbarum polysaccharides (LBPs) were constructed, combined with a variety of chemometric methods, to evaluate the differences of LBPs from different origins and identify the authenticity of commercial samples. Sixteen batches of Lycium barbarum polysaccharides (LBPs) from different areas were obtained by the method of water extraction and alcohol precipitation, which were partially hydrolyzed by trifluoroacetic acid. The PMP pre-column derivatization-HPLC method was applied to analyse the monosaccharides and oligosaccharides in the partial hydrolysates of LBPs. The similarity of samples of different origins was calculated by the “similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(TCM)”software. And the fingerprints were further evaluated by chemometric methods including cluster analysis (HCA), principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The results showed that the partial acid hydrolysis products of LBPs contained seven monosaccharides, galacturonic acid glycans of DP 2~4 and three kinds of disaccharides. The similarities of 16 batches of LBPs of different origins were high ranging from 0.911 to 0.997. HCA, PCA and PLS-DA all divided LBPs from different origins into three categories, and the classification results had certain correlation with the origins. The three iconic components that influenced the differences between origins were screened out by PLS-DA, which were glucose (Glc), arabinose (Ara) and galactose (Gal), respectively. The established fingerprint in this paper could be used for the quality control of LBPs.
- Lycium barbarum polysaccharides,
- partial acid hydrolysis,
- fingerprinting,
- chemometrics,
- quality control

FullText(HTML)

References (26)

References

[1]	Zhang Q, Chen W, Zhao J, et al. Functional constituents and antioxidant activities of eight Chinese native goji genotypes[J]. Food Chemistry,2016,200(Jun. 1):230−236.
[2]	夏娜, 赵丽凤. 黑果枸杞功能性成分抗氧化活性及对线粒体的保护作用研究[J]. 食品工业科技,2014,35(22):162−166. [Xia N, Zhao L F. Study on the antioxidant activity and protective effect on mitochondria of Lycium ruthenicum functional ingredients[J]. Science and Technology of Food Industry,2014,35(22):162−166.
[3]	Hempel J, Schdle C N, Sprenger J, et al. Ultrastructural deposition forms and bioaccessibility of carotenoids and carotenoid esters from goji berries (Lycium barbarum L.)[J]. Food Chemistry,2017,218(1):525−533.
[4]	Liu W J, Jiang H F, Rehman F U L, et al. Lycium barbarum polysaccharides decrease hyperglycemia-aggravated ischemic brain injury through maintaining mitochondrial fission and fusion balance[J]. International Journal of Biological Sciences,2017,13(7):901−910. doi: 10.7150/ijbs.18404
[5]	Zhao Q, Li J, Yan J, et al. Lycium barbarum polysaccharides ameliorates renal injury and inflammatory reaction in alloxan-induced diabetic nephropathy rabbits[J]. Life Sciences,2016,157(15):82−90.
[6]	Sun X, Lv Y, Huang L, et al. Pro-inflammatory cytokines serve as communicating molecules between the liver and brain for hepatic encephalopathy pathogenesis and Lycium barbarum polysaccharides protection[J]. Journal of Ethnopharmacology,2020,248(Feb. 10):112357−112357.
[7]	黄琳娟, 田庚元, 齐春会, 等. 枸杞子糖缀合物LbGp4糖链的结构及其免疫活性的研究[J]. 高等学校化学学报,2001,22(3):407−411. [Huang L J, Tian G Y, Qi C H, et al. Structure elucidation and immunoactivity studies of glycan of glycoconjugate LbGp4 isolated from the fruit of Lycium barbarum L[J]. Chemical Journal of Chinese Universities,2001,22(3):407−411. doi: 10.3321/j.issn:0251-0790.2001.03.010
[8]	Harunobu A, Sun B X, Carmia B. Lycium barbarum (goji) juice improves in vivo antioxidant biomarkers in serum of healthy adults[J]. Nutrition Research,2009,29(1):19−25. doi: 10.1016/j.nutres.2008.11.005
[9]	李良, 金文娟. 枸杞多糖含量测定方法的比较分析[J]. 食品研究与开发,2016,37(11):143−146. [Li L, Jin W J. Comparative analysis methods of Lycium barbarum polysaccharide content determination[J]. Food Research and Development,2016,37(11):143−146. doi: 10.3969/j.issn.1005-6521.2016.11.033
[10]	Zhang H, Wang J N, Chen Y, et al. Establishing the chromatographic fingerprint of traditional Chinese medicine standard decoction based on quality by design approach: A case study of licorice[J]. Journal of Separation Science,2019,42(6):1144−1154. doi: 10.1002/jssc.201800989
[11]	Liu X Y, Jiang W W, Su M, et al. Quality evaluation of traditional Chinese medicines based on fingerprinting[J]. Journal of Separation Science,2020,43(1):6−17. doi: 10.1002/jssc.201900365
[12]	符梦凡. 枸杞多糖的分离提取及质量鉴定研究[D]. 杭州: 浙江大学, 2018. Fu M F. Studies on the extraction and quality determination of Lycium barbarum polysaccharide[D]. Hangzhou: Zhejiang University, 2018.
[13]	蒋梅, 谭丽蓉, 黄晓洁, 等. 枸杞子多糖柱前衍生HPLC指纹图谱分析[J]. 中国实验方剂学杂志,2014,20(22):53−56. [Jiang M, Tan L R, Huang X J, et, al. Pre-column derivatives HPLC fingerprint of Lycii fructus polysaccharides[J]. Chinese Journal of Experimental Traditional Medical Formulae,2014,20(22):53−56.
[14]	徐金楠, 刘玮, 刘春晶, 等. 不同枸杞中多糖含量与结构特征的对比研究[J]. 中国食品学报,2015,15(4):233−239. [Xu J N, Liu W, Liu C J, et al. Comparative study of the content and structural features of polysaccharides from different Lycium[J]. Journal of Chinese Institute of Food Science and Technology,2015,15(4):233−239.
[15]	Liu W, Xu J N, Zhu R, et al. Fingerprinting profile of polysaccharides from Lycium barbarum using multiplex approaches and chemometrics[J]. International Journal of Biological Macromolecules,2015,78(Jul):230−237.
[16]	陈欢欢, 赵峡, 栾晓红, 等. 电喷雾质谱在寡糖序列分析中的应用[J]. 高等学校化学学报,2015,36(1):1−8. [Chen H H, Zhao X, Luan X H, et al. Application of electrospray tandem mass spectrometry in sequence analysis of oligosaccharides[J]. Chemical Journal of Chinese Universities,2015,36(1):1−8.
[17]	Bai W D, Fang X D, Zhao W H, et al. Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography[J]. Journal of Food and Drug Analysis,2015,23(4):645−651. doi: 10.1016/j.jfda.2015.04.011
[18]	Wong T L, Li L F, Zhang J X, et al. Oligosaccharide-marker approach for qualitative and quantitative analysis of specific polysaccharide in herb formula by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry: Dendrobium officinale, a case study[J]. Journal of Chromatography A,2019,1607(6):460338−460338.
[19]	肖颖, 吴梦琪, 张文清, 等. 茯苓多糖HPLC指纹图谱与免疫活性的相关分析[J]. 华东理工大学学报(自然科学版),2020,46(5):672−679. [Xiao Y, Wu M Q, Zhang W Q, et al. Correlation analysis between HPLC fingerprint of polysaccharides from poria cocos and immunological activity[J]. Journal of East China University of Science and Technology(Natural Science Edition),2020,46(5):672−679.
[20]	王园, 吴梦琪, 张文清, 等. HPLC结合化学计量学的枸杞多糖指纹图谱研究[J]. 中药材,2019,42(7):1572−1577. [Wang Y, Wu M Q, Zhang W Q, et al. Study on fingerprint of Lycium barbarum polysaccharides by HPLC combined with chemometrics[J]. Journal of Chinese Medicinal Materials,2019,42(7):1572−1577.
[21]	潘建义, 成浩, 王丽鸳, 等. 基于化学指纹图谱的茶树新品系丽早香识别研究[J]. 浙江农业学报,2016,28(10):1724−1729. [Pan J Y, Cheng H, Wang L Y, et al. Identification and characterization of new tea line Lizaoxiang based on chemical fingerprint[J]. Acta Agricluturae Zhejiangensis,2016,28(10):1724−1729. doi: 10.3969/j.issn.1004-1524.2016.10.14
[22]	Wang H, Zhao J, Li D M, et al. Comparison of polysaccharides of Haliotis discus hannai and Volutharpa ampullacea perryi by PMP-HPLC-MSn analysis upon acid hydrolysis[J]. Carbohydrate Research,2015,415(Oct. 13):48−53.
[23]	鲁姣姣, 艾春青, 温成荣, 等. PMP-HPLC-MSn法分析弱酸降解鲍鱼性腺多糖产生的寡糖[J]. 食品科学,2016,37(16):112−116. [Lu J J, Ai C Q, Wen C R, et al. Analysis of oligosaccharides released from weak acid hydrolysis of abalone gonad polysaccharide using PMP-HPLC-MSn[J]. Food Science,2016,37(16):112−116. doi: 10.7506/spkx1002-6630-201616018
[24]	李文德, 陈尚卫, 杨浩, 等. 基于柱前衍生化反相色谱分析的枸杞多糖产品的掺杂检测[J]. 中国食品添加剂,2019,30(12):181−188. [Li W D, Chen S W, Yang H, et al. Adulteration detection of Lycium barbarum polysaccharide products based on per-column derivatization reversed-phase liquid chromatography[J]. China Food Additives,2019,30(12):181−188.
[25]	Sun M, Yan D H, Yang X L, et al. Quality assessment of crude and processed Arecae semen based on colorimeter and HPLC combined with chemometrics methods[J]. Journal of Separation Science,2017,40(10):2151−2160. doi: 10.1002/jssc.201700006
[26]	Li Y Q, Kong D X, Wu H. Comprehensive chemical analysis of the flower buds of five Lonicera species by ATR-FTIR, HPLC-DAD, and chemometric methods[J]. Revista Brasileira de Farmacognosia,2018,28(5):533−541. doi: 10.1016/j.bjp.2018.06.007