Determination of Chloramphenicol, Thiamphenicol, Florfenicol and Florfenicol Amine Residues in Beeswax by Solid Phase Extraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry

LEI Meikang; HOU Jianbo; PENG Fang; ZHANG Wenhua; ZHU Zitong; ZHANG Yingjun; CHEN Yujiao; XIE Wen

doi:10.13386/j.issn1002-0306.2020100039

Volume 42 Issue 17

Aug. 2021

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Science and Technology of Food Industry > 2021 > 42(17): 241-246. > DOI: 10.13386/j.issn1002-0306.2020100039

LEI Meikang, HOU Jianbo, PENG Fang, et al. Determination of Chloramphenicol, Thiamphenicol, Florfenicol and Florfenicol Amine Residues in Beeswax by Solid Phase Extraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(17): 241−246. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100039.

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Determination of Chloramphenicol, Thiamphenicol, Florfenicol and Florfenicol Amine Residues in Beeswax by Solid Phase Extraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry

LEI Meikang^{1, 2,},
HOU Jianbo¹,
PENG Fang^{1, 2},
ZHANG Wenhua¹,
ZHU Zitong^{1, 2},
ZHANG Yingjun²,
CHEN Yujiao²,
XIE Wen^1, ,

1.
Technical Center of Hangzhou Customs, Hangzhou 310016, China
2.
The Comprehensive Technology and Service Center of Quzhou Customs, Quzhou 324003, China

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Received Date: October 11, 2020
Available Online: July 04, 2021

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Abstract

Abstract

A method based on solid-phase extraction and high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry(SPE-HPLC-MS/MS) had been proposed for the determination of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine residues in beeswax. The sample was pre dissolved with n-hexane and then extracted with water, purified the Waters Oasis MCX micro-column and separated on the InertSustain Swift C₁₈ column (100 mm×2.1 mm, 1.9 μm). The determination was carried out with eletrospray ion source and multiple reaction monitoring (MRM) mode and the corresponding quantification was performed by employing the method of internal standards. The calibration curve of mixed standard solution showed a good linearity in the concentration rang from 0.1 to 10 ng/mL with the correlation coefficients (r) more than 0.99. The limits of quantification (S/N>10) for antibiotics were 0.1 μg/kg. The recovery was 65.0% to 113.20% in the spiked concentration 0.1, 0.3 and 1.0 μg/kg, with the relative standard deviations (RSD, n=6) lower than 20%.The method was applied to detect 15 beeswax samples, and the results showed that chloramphenicol residues were detected in 4 batches of samples. The method is rapid, sensitive and accurate, and could be applied for the qualitative and quantitative analysis of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine residues antibiotics in beeswax.

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References

[1]	徐国钧, 宋鑫. 世界主要蜂蜡出口国的国际竞争力分析[J]. 蜜蜂杂志,2020,40(4):7−10. [Xu Guo-jun, Song Xin. Analysis on international competitiveness of the world's leading exporter of beewax[J]. Journal of Bee,2020,40(4):7−10.
[2]	王静琳, 汪燕, 马振刚. 综述蜂蜡的应用[J]. 蜜蜂杂志,2019,12:9−12. [Wang Jing-lin, Wang Yan, Ma Zhen-gang. Review of the application of beeswax[J]. Journal of Bee,2019,12:9−12.
[3]	雷美康, 彭芳, 祝子铜, 等. 高效液相色谱-串联质谱法测定蜂蜡中硝基呋喃类代谢物[J]. 分析试验室,2015,34(10):1174−1177. [Lei Mei-kang, Peng Fang, Zhu Zi-tong, et al. Determination of metabolites of nitrofuran antibiotics in beeswaxusing high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Analysis Laboratory,2015,34(10):1174−1177.
[4]	M D Navarro-Hortal, Orantes-Bermejo F J , C Sánchez-González, et al.Industrial-scale decontamination procedure effects on the content of acaricides, heavy metals and antioxidant capacity of beeswax[J]. Molecules 2019, 24(8): 1518.
[5]	Silvia V, Ares ana maría, Bernal josé luis, et al. Fast determination of neonicotinoid insecticides in beeswax by ultra-high performance liquid chromatography-tandem mass spectrometry using an enhanced matrix removal-lipid sorbent for clean-up[J]. Microchemical Journal,2018,142:70−77. doi: 10.1016/j.microc.2018.06.020
[6]	Muhammad jamal, Muhmmad asif aziz, Muhammad naeem, et al. Detection of flumethrin acaricide residues from honey and beeswax using high performance liquid chromatography (HPLC) technique[J]. Journal of King Saud University - Science,2020,32(3):2229−2235. doi: 10.1016/j.jksus.2020.02.035
[7]	Wallner, K. Varroacides and their residues in bee products[J]. Apidologie,1999,30:235−248. doi: 10.1051/apido:19990212
[8]	Issa M M, Taha S M, El-marsafy A M, et al. Acetonitrile-ethyl acetate based method for the residue analysis of 373 pesticides in beeswax using LC-MS/MS and GC-MS/MS[J]. Journal of Chromatography B,2020,1145:122106. doi: 10.1016/j.jchromb.2020.122106
[9]	Medina-dzul K, Medina-peralta S, Carrera-figueiras C, et al. Matrix solid-phase dispersion extraction of organophosphorous pesticides from beeswax[J]. International Journal of Environmental Analytical Chemistry,2017:1−10.
[10]	陈芳, 李丹, 张金振, 等. 气相色谱微池电子捕获检测器测定蜂蜡中6种杀螨剂药物残留[J]. 食品科学,2010,31(18):244−247. [Chen Fang, Li Dan, Zhang Jin-zhen, et al. Determination of six acaricide residues in beeswax by gas chromatography coupled with a micro-electron capture detector[J]. Food Science,2010,31(18):244−247.
[11]	张伟玮, 曾明华, 许世富, 等. 高效液相色谱-串联质谱法同时测定鸡肉中氯霉素药物及其代谢物残留方法的建立[J]. 安徽农业大学学报,2012,39(4):641−645. [Zhang Wei-wei, Zeng Hua-ming, Xu Shi-fu, et al. Development of method for simultaneous determination of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine residues in chicken tissues by LC-MS/MS[J]. Journal of Anhui Agricultural University,2012,39(4):641−645.
[12]	Wang Hao, Zhou Xiang-Juan, Liu Yan-Qin, et al. Simultaneous determination of chloramphenicol and aflatoxin M1 residues in milk by triple quadrupole liquid chromatography tandem mass spectrometry[J]. Journal of Agricultural and Food Chemistry,2011,59:3532−3538. doi: 10.1021/jf2006062
[13]	Rodziewicz L, Zawadzka I. Rapid determination of chloramphenicol residues in milk powder by liquid chromatography–elektrospray ionization tandem mass spectrometry[J]. Talanta,2008,75:846−850. doi: 10.1016/j.talanta.2007.12.022
[14]	祝子铜, 雷美康, 彭芳, 等. 快速溶剂萃取-凝胶渗透色谱净化-LC/MS/MS结合测定蜂花粉中硝基咪唑类药物[J]. 药物分析杂志,2016(3):522−529. [Zhu Zi-tong, Lei Mei-kang, Peng Fang, et al. Determination of nitroimidazoles residues in bee pollen using LC-MS/MS coupled with accelerated solvent extraction and gel permeation chromatographic purification[J]. Chinese Journal of Pharmaceutical Analysis,2016(3):522−529.
[15]	GB 31650-2019 食品安全国家标准食品中兽药最大残留限量[S]. 北京:中国标准出版社, 2019. GB 31650-2019 National food safety standard—Maximum residue limits for veterinary drugs in foods[S]. Beijing: China Standards Press, 2019.
[16]	李莹莹, 宋永青, 赵榕, 等. 液质联用检测鱼肉中氟苯尼考和氟苯尼考胺的残留量[J]. 食品科学,2010,31(12):219−222. [Li Ying-ying, Song Yong-qing, Zhao Rong, et al. HPLC-MS/MS determination of florfenicoland florfenicol amine residues in fish[J]. Food Science,2010,31(12):219−222.
[17]	张凤清, 王岩松, 范世华, 等. 高效液相色谱-串联质谱法测定动物肌肉组织中氯霉素、甲砜霉素和氟苯尼考残留量[J]. 食品科学,2010,31(8):248−251. [Zhang Feng-qing, Wang Yan-song, Fan Shi-hua, et al. High performance liquid chromatography-tandem mass spectrometric determination of chloramphenicol, thiamphenicoland florfenicol residues in animal muscles[J]. Food Science,2010,31(8):248−251.
[18]	Muhammad imran, Fazal-e- habib, Saima majeed, et al. LC-MS/MS-based determination of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in poultry meat from the Punjab-Pakistan[J]. Food Addit Contam Part A Chem Anal Control Expo Risk Assess,2018,35(8):1530−1542. doi: 10.1080/19440049.2018.1463569
[19]	Xie Xing, Wang Bo, MaodaPang, et al. Quantitative analysis of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in eggs via liquid chromatography-electrospray ionization tandem mass spectrometry[J]. Food Chemistry,2018,269:542−548. doi: 10.1016/j.foodchem.2018.07.045
[20]	Fabianobarreto, Cristinaribeiro, Rodrigobarcellos hoff, et al. Determination of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in poultry, swine, bovine and fish by liquid chromatography-tandem mass spectrometry[J]. Journal of Chromatography A,2016,1449:48−53. doi: 10.1016/j.chroma.2016.04.024
[21]	1025号公告-23-2008 动物源食品中磺胺类药物残留检测液相色谱—串联质谱法[S]. 北京:中国标准出版社, 2008. Announcement no. 1025-23-2008. Determination of sulfonamides residues in edible tissues of animal Liquid chromatography-tandem mass spectrometry[S]. Beijing: China Standards Press, 2008.
[22]	Shizuka, Saito-Shida, Kouji, et al. Determination of total florfenicol residues as florfenicol amine in bovine tissues and eel by liquid chromatography-tandem mass spectrometry using external calibration.[J]. Journal of Chromatography B,2019,1109:37−44. doi: 10.1016/j.jchromb.2019.01.018
[23]	Orlando, Adilson E, Roque C, et al. UPLC-MS/MS determination of florfenicol and florfenicol amine antimicrobial residues in tilapia muscle[J]. Journal of Chromatography B,2016,.1035:8−15. doi: 10.1016/j.jchromb.2016.09.013
[24]	方海仙, 耿慧春, 梅文泉, 等. 气相色谱法测定农产品中农药残留的基质效应研究[J]. 食品安全质量检测学报,2018,9(14):3770−3779. [Fang Hai-xian, Geng Hui-chun, Mei Wen-quan, et al. Study on matrix effect of determination of pesticide residues in agricultural products by gas chromatography[J]. Journal of Food Safety and Quality,2018,9(14):3770−3779. doi: 10.3969/j.issn.2095-0381.2018.14.037
[25]	Li Jian-bo, Hu Yu-jie, Wu Ya-jin, et al. Pharmacokinetics of palmatine in rat after oral and intravenous administration by UPLC-MS/MS[J]. Acta Chromatographica,2021,33(1):25−29.
[26]	Hana bavlovi piskáková, Iestad E L, Nela váňová, et al. Electromembrane extraction of anthracyclines from plasma: Comparison with conventional extraction techniques[J]. Talanta,2021,223:121748. doi: 10.1016/j.talanta.2020.121748
[27]	吕冰峰, 刘敏, 裴新荣. 2015~2018年蜂蜜国家食品安全监督抽检结果分析[J]. 食品安全质量检测学报,2019,23(12):8164−8170. [Lv Bing-Feng, Liu Min, Pei Xin-rong. Results analysis of the national food safety supervision and sampling inspection of honey from 2015 to 2018[J]. Journal of Food Safety and Quality,2019,23(12):8164−8170.
[28]	Shimshoni J A, Sperling R, Massarwa M, et al. Pesticide distribution and depletion kinetic determination in honey and beeswax: Model for pesticide occurrence and distribution in beehive products[J]. PLoS One,2019,14(2):e0212631. doi: 10.1371/journal.pone.0212631
[29]	Agrebi N E, Traynor K, Wilmart O, et al. Pesticide and veterinary drug residues in Belgian beeswax: Occurrence, toxicity, and risk to honey bees[J]. Science of The Total Environment,2020,745:141036. doi: 10.1016/j.scitotenv.2020.141036
[30]	Vijayakumar bommuraj, Chen Y, Klein H, et al. Pesticide and trace element residues in honey and beeswax combs from Israel in association with human risk assessment and honey adulteration[J]. Food Chemistry,2019,299:125123. doi: 10.1016/j.foodchem.2019.125123

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