Improving Effect of Chitotriose Guanidine Hydrochloride on Insulin Resistance Cell Model

ZHENG Anna; ZHAO Mengyao; YOU Jiangshan; PAN Xiaoxu; ZHAO Liming

doi:10.13386/j.issn1002-0306.2020100020

Volume 42 Issue 19

Sep. 2021

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Science and Technology of Food Industry > 2021 > 42(19): 350-356. > DOI: 10.13386/j.issn1002-0306.2020100020

ZHENG Anna, ZHAO Mengyao, YOU Jiangshan, et al. Improving Effect of Chitotriose Guanidine Hydrochloride on Insulin Resistance Cell Model[J]. Science and Technology of Food Industry, 2021, 42(19): 350−356. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100020.

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Improving Effect of Chitotriose Guanidine Hydrochloride on Insulin Resistance Cell Model

State Key Laboratory of Bioreactor Engineering, Research and Development Centre of Separation and Extraction Technology in Fermentation Industry, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China

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Received Date: October 11, 2020
Available Online: August 11, 2021

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Abstract

Abstract

In order to evaluate the ability of chitotriose guanidine hydrochloride to improve insulin resistance, this study used high-sugar and high-fat to induce HepG2 cells to establish an insulin resistance cell model, and intervened the cell model with different concentrations and guanidine substituted chitotriose guanidine hydrochloride. Then, indicators such as glucose uptake of model cells were detected. The results showed that 300 µg/mL and 600 µg/mL chitotriose guanidine hydrochloride could significantly increase the glucose uptake of the model cells (P<0.05), while 600 µg/mL displayed a superior effect. Chitotriose guanidine hydrochloride with different guanidine substitution degrees could significantly increase the glucose uptake of model cells (P<0.05), while 78% guanidine substitution had the best effect. Chitotriose guanidine hydrochloride with 600 µg/mL and 78% guanidine substitution degree significantly increased the glucose uptake of the model cells and glycogen content (P<0.05), while reducing the iNOS activity in the cells (P<0.05), and exhibiting a beneficial effect on the insulin-resistant cell model, which provides a theoretical basis for the development of new functional foods that have a good effect in type 2 diabetes intervention.
- type 2 diabetes,
- improving effect,
- chitotriose guanidine hydrochloride,
- insulin resistance

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