Separation and Purification of Kudinoside D and Its Nanoparticle Preparation and Characterization

HU Xiatian; ZHANG Fengqing; YU Min

doi:10.13386/j.issn1002-0306.2020090324

Volume 42 Issue 19

Sep. 2021

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Science and Technology of Food Industry > 2021 > 42(19): 15-20. > DOI: 10.13386/j.issn1002-0306.2020090324

HU Xiatian, ZHANG Fengqing, YU Min. Separation and Purification of Kudinoside D and Its Nanoparticle Preparation and Characterization[J]. Science and Technology of Food Industry, 2021, 42(19): 15−20. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090324.

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Separation and Purification of Kudinoside D and Its Nanoparticle Preparation and Characterization

1.
College of Chemistry and Life Sciences, Changchun University of Technology, Changchun 130012, China
2.
Jinlin Provincial Academy of Traditional Chinese Medicine, Changchun 130118, China

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Received Date: September 29, 2020
Available Online: August 08, 2021

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Abstract

Abstract

Objective: To improve the water solubility of Kudinoside D. Methods: Using atmospheric column chromatography, semi-preparative high performance liquid chromatography and other means to separate and purify saponins from Ilex kudingcha C.J.Tseng, the compound Kudinoside D was obtained. Using polyglutamic acid (γ-PGA) and L-phenylalanine ethyl ester (L-PAE) as raw materials, Kudinoside D nanoparticles were prepared by precipitation method and dialysis method respectively. The physical and chemical properties of nanoparticles were characterized by transmission electron microscopy and dynamic nanoparticle size analyzer, and their in vitro drug release characteristics were investigated by dialysis. Results: Compared with the precipitation method, the encapsulation efficiency and drug loading of Kudinoside D nanoparticles prepared by the dialysis method were increased by 1.5 and 4.5 times, respectively, the encapsulation rate reached 65.46%, and the drug loading reached 13.24%. Kudinoside D nanoparticles had a regular spherical shape with an average particle size of (75±25) nm and a Zeta potential of 33.7. Conclusion: In vitro drug release experiments show that Kudinoside D nanoparticles can significantly improve the water solubility of the drug and have a good sustained release effect in vitro.
- Kudinoside D,
- γ-PGA-PAE,
- nanoparticles,
- characterization,
- isolation and purification

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