Protein Components of P<italic/>rotein in <i>Colla Corii Asini</i>

Feng LIAO; Yumei FAN; Hang TIE; Ning SU

doi:10.13386/j.issn1002-0306.2020090189

Volume 42 Issue 10

May 2021

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Science and Technology of Food Industry > 2021 > 42(10): 122-129. > DOI: 10.13386/j.issn1002-0306.2020090189

LIAO Feng, FAN Yumei, TIE Hang, et al. Protein Components of Protein in Colla Corii Asini [J]. Science and Technology of Food Industry, 2021, 42(10): 122−129. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090189.

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Protein Components of Protein in Colla Corii Asini

1.
National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co., Ltd., Liaocheng 252201, China
2.
Chinese Academy of Inspection and Quarantine, Beijing 100176, China

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Received Date: September 17, 2020
Available Online: March 15, 2021

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Abstract

Abstract

To explore the pharmacological activity of donkey hide gelatin related protein components, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Orbitrap Fusion Lumos Tribrid were used to reveal the composition of Colla Corii Asini (CCA), which would provide a basis for the exploration of protein components related to the pharmacological activity of CCA and further development of CCA. The results showed that the molecular weight was distributed in the range of 10~250 kDa. By comparing with the equine protein library, 316 proteins were retrieved. Results found that CCA contained a variety of active proteins, including leucine-rich proteoglycans, such as decorin, biglycan, Lumican, fibromodulin, and other active substances such as collagen and immunoglobulins. Further bioinformatics analysis of proteins in CCA revealed that these proteins are participate in signal pathways such as systemic lupus erythematosus, protein digestion and absorption, ECM receptor interaction, viral carcinogenesis, hypertrophic cardiomyopathy, and dilated cardiomyopathy. The screening of pharmacologically active ingredients provides a theoretical basis. This results would provide a theoretical basis for the screening and further development of pharmacological active components of CCA.
- Colla Corii Asini,
- Orbitrap Fusion Lumos Tribrid,
- protein fraction,
- bioinformatics

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References

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