Mutation Breeding and Enzymatic Properties of Cold-adapted Chitinolytic Strain <i>Photobacterium </i>sp. from Marine

Ligong CHEN; Jiawei WU; Jinping ZHANG; Qingfang ZHANG; Naiyu CHI; Xiaohui WANG; Mengyu WANG

doi:10.13386/j.issn1002-0306.2020090077

Volume 42 Issue 10

May 2021

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Science and Technology of Food Industry > 2021 > 42(10): 105-111. > DOI: 10.13386/j.issn1002-0306.2020090077

CHEN Ligong, WU Jiawei, ZHANG Jinping, et al. Mutation Breeding and Enzymatic Properties of Cold-adapted Chitinolytic Strain Photobacterium sp. from Marine[J]. Science and Technology of Food Industry, 2021, 42(10): 105−111. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090077.

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Mutation Breeding and Enzymatic Properties of Cold-adapted Chitinolytic Strain Photobacterium sp. from Marine

Dalian Key Laboratory of Synthetic Biology, Liaoning Marine Microbial Engineering Technology Research Center, School of Life Science and Biotechnology, Dalian University, Dalian 116600, China

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Received Date: September 09, 2020
Available Online: March 15, 2021

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Abstract

To study the physicochemical properties of the strain Photobacterium sp. LG-1 and improve its enzyme production activity, BIOLOG was used to carry out physiological and biochemical experiments. Parallel breeding through ultraviolet mutagenesis, chemical mutagenesis and compound mutagenesis. Research on its enzymatic properties and antibacterial properties. The enzyme activity of this strain reached 2.690 U/mL after compound mutagenesis, and the increasing rate of enzyme activity was 139.78%. The optimum temperature was 35 °C, and the chitinase still showed catalytic activity at 0 °C. The optimum pH was 8.0, and it had stability in an alkaline environment. Ca²⁺, K⁺, Mg²⁺, Cu²⁺ and Na⁺ could promote chitinase activity. LG-1 had inhibitory activity against Rhizopus, Aspergillus niger, Mucor and rot of Corn .
- Photobacterium,
- cold-adapted chitinase,
- physiological and biochemical,
- enzymatic properties,
- antifungal activity

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