Protective Effect of <i>Forsythia suspensa</i> Leaf Extracts on Heat-stressed Rats

WANG Lingzhi; TANG Jiankai; WANG Shuheng; JIN Xiang; ZHANG Liwei

doi:10.13386/j.issn1002-0306.2020090052

Volume 42 Issue 17

Aug. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(17): 343-349. > DOI: 10.13386/j.issn1002-0306.2020090052

WANG Lingzhi, TANG Jiankai, WANG Shuheng, et al. Protective Effect of Forsythia suspensa Leaf Extracts on Heat-stressed Rats[J]. Science and Technology of Food Industry, 2021, 42(17): 343−349. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090052.

Citation:

PDF (1366 KB)

Protective Effect of Forsythia suspensa Leaf Extracts on Heat-stressed Rats

WANG Lingzhi^{1, 2,},
TANG Jiankai^{1, 2},
WANG Shuheng^{1, 2},
JIN Xiang^{1, 2},
ZHANG Liwei^1, ,

1.
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China
2.
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China

More Information

Received Date: September 09, 2020
Available Online: July 03, 2021

Graphical Abstract

Abstract

Abstract

To study the protective effect of Forsythia suspensa leaf extract (FSLE) on heat-stressed rats, thirty rats were randomly divided into control group, heat stress group, and FSLE low-dose group, FSLE medium-dose group, and FSLE high-dose group, artificial intelligent climatic chamber was uesd to establish the heat stress model of rats. At the end of the heat stress experiment, the growth performance, rectal temperature, organ index and other indicators of the rats in each group were analyzed. The pathological changes of the rats ileum and liver tissues were observed, and the contents of corticosterone (CORT) and adrenocorticotropic hormone (ACTH) in the serum were detected. The results showed that compared with the model group, the immune organ index of the FSLE high-dose group increased significantly(P<0.05). The levels of CORT and ACTH in serum were significantly reduced(P<0.05), and the injury of rats ileum and liver tissue was significantly improved. This study showed that FSLE had a certain protective effect on heat-stressed rats, and it could be used for the prevention of heat stroke.
- Forsythia suspensa leaf extracts,
- heat stress,
- immune organ index,
- liver injury

FullText(HTML)

References (35)

References

[1]	Cheng K, Yan E, Song Z, et al. Protective effect of resveratrol against hepatic damage induced by heat stress in a rat model is associated with the regulation of oxidative stress and inflammation[J]. Journal of Thermal Biology,2019,82:70−75. doi: 10.1016/j.jtherbio.2019.03.012
[2]	郭晨, 刘林峰, 向奕, 等. L-茶氨酸改善热应激引起的小鼠组织损伤和氧化逆境[J]. 茶叶科学,2017,37(1):17−24. [Guo C, Liu L F, Xiang Y, et al. L-theanine attenuates tissue and oxidative damages in heat stressed mice[J]. Journal of Tea Science,2017,37(1):17−24. doi: 10.3969/j.issn.1000-369X.2017.01.003
[3]	Leon L R, Dineen S, Blaha M D, et al. Attenuated thermoregulatory, metabolic and liver acute phase protein response to heat stroke in TNF-receptor knockout mice[J]. American Journal of Physiology-Regulatory Integrative and Comparative Physiology,2013,305(12):1421−1432. doi: 10.1152/ajpregu.00127.2013
[4]	Wang D, Cai M, Wang T, et al. Theanine supplementation prevents liver injury and heat shock response by normalizing hypothalamic-pituitaryadrenal axis hyperactivity in mice subjected to whole body heat stress[J]. Journal of Functional Foods,2018:45.
[5]	Hao Y, Feng Y, Li J, et al. Role of MAPKs in HSP70's protection against heat stress-induced injury in rat small intestine[J]. BioMed Research International,2018:1−10.
[6]	韦大文, 王守东, 孙再玲. 三仙信力胶囊对热应激小鼠病理形态学的观察[J]. 中国中药杂志,2006(6):485−486. [Wei D W, Wang S D, Sun Z L. Observation of Sanxian Xinli capsule on pathologic morphology in superheat mice[J]. China Journal of Chinese Materia Medica,2006(6):485−486. doi: 10.3321/j.issn:1001-5302.2006.06.014
[7]	Erfani M, Ghazi Tabatabaei Z, Sadigh-Eteghad S, et al. Rosa canina L. methanolic extract prevents heat stress-induced memory dysfunction in rat[J]. Experimental Physiology,2019,104(10):1544−1554. doi: 10.1113/EP087535
[8]	邬堂春, 陈胜, 肖成峰, 等. 急性热应激对飞行员免疫活性细胞的影响研究[J]. 职业卫生与病伤,1999,14(2):3−5. [Wu T C, Chen S, Xiao C F, et al. Study on the effect of acute heat stress on the immune active cells of pilots[J]. Journal of Occupational Health and Damage,1999,14(2):3−5.
[9]	Aronow W S, Aan C. Elderly nursing home patients with congestive heart failure after myocardial infarction living in New York city have a higher prevalence of mortality in cold weather and warm weather months[J]. Journal of Gerontology: Medical Sciences,2004,59(2):146−147. doi: 10.1093/gerona/59.2.M146
[10]	郑艺欣, 李奕君, 林埔, 等. 野菊花水提物对果蝇热耐受能力的影响[J]. 食品与机械,2016,32(9):141−143, 171. [Zheng Y X, Li Y J, Lin P, et al. Effect of water extract from Flos Chrysanthemi Indici on heat tolerance of drosophila melanogaster[J]. Food & Machinery,2016,32(9):141−143, 171.
[11]	王慧玲, 崔伟, 秦鑫, 等. 鱼腥草对致热大鼠下丘脑c AMP和腹中隔区精氨酸加压素含量的影响[J]. 中国临床药理学与治疗学,2007,12(1):78−81. [Wang H L, Cui W, Qin X, et al. Effects of Yuxingcao injection on contents of cAMP in hypothalamus and AVP in ventral septal area in fever rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics,2007,12(1):78−81. doi: 10.3969/j.issn.1009-2501.2007.01.018
[12]	张玖, 戴艳玲. 金银花及其水提物对果蝇耐热能力的影响[J]. 中国野生植物资源,1997,16(3):33−34. [Zhang J, Dai Y L. Effects of Flos Lonicerae and its water extract on the heat resistance of Drosophila melanogaster[J]. Chinese Wild Plant Resources,1997,16(3):33−34.
[13]	Kim K J, Yoon K Y, Hong H D, et al. Role of the red ginseng in defense against the environmental heat stress in sprague dawley rats[J]. Molecules,2015,20(11):20240−20253. doi: 10.3390/molecules201119692
[14]	赵韶华, 刘敏彦, 王玉峰, 等. HPLC-ELSD法同时测定连翘叶中齐墩果酸和熊果酸[J]. 食品科学,2012,33(2):224. [Zhang X H, Liu M Y, Wang Y F, et al. Simultaneous determination of oleanolic acid and ursolic acid in the leaves of Forsythia suspensa by HPLC-ELSD[J]. Food Science,2012,33(2):224.
[15]	国家中医药管理局《中华本草》编委会. 中华本草: 第十六卷[M]. 上海: 上海科技出版社, 1999: 1157−1159. Editorial board of Chinese materia medica by the State Administration of traditional Chinese medicine. Chinese materia medica: Volume 16 [M]. Shanghai: Shanghai Science and Technology Press, 1999: 1157-1159.
[16]	侯改霞, 杨建雄. 连翘叶提取物对实验小鼠的降脂保肝作用研究[J]. 河南大学学报(自然科学版),2010,40(5):504−506. [Hou G X, Yang J X. Effects of Extracts from Forsythia suspense leaves on modulating blood lipids and protecting liver of hyperlipidemic mice[J]. Journal of Henan University (Natural Science),2010,40(5):504−506.
[17]	贾东升, 李荣乔, 谢晓亮, 等. 连翘叶不同溶剂提取物体外抗氧化活性研究[J]. 食品研究与开发,2016,37(2):14−18. [Jia D X, Li R J, Xie X L, et al. Study on antioxidant activity of different solvents extracts of Forsythia suspensa leaves[J]. Food Research and Development,2016,37(2):14−18. doi: 10.3969/j.issn.1005-6521.2016.02.004
[18]	王婷婷, 张蕻, 杨志敏, 等. 连翘及连翘叶抑菌抗炎活性及其主要化学成分研究[J]. 中国药物与临床,2019,19(14):2380−2381. [Wang T T, Zhang H, Yang Z M, et al. Study on antibacterial and anti-inflammatory activities and main chemical constituents of Forsythia suspensa and its leaves[J]. Chinese Remedies & Clinics,2019,19(14):2380−2381.
[19]	刘静, 杨建雄. 连翘叶茶对小鼠非特异性免疫及应激作用的试验研究[J]. 榆林学院学报,2006,16(2):45−47. [Liu J, Yang J X. The Experimental study of the immunological function and anti-stress of Forsythia Suspensa leaves tea on mice[J]. Journal of Yulin University,2006,16(2):45−47. doi: 10.3969/j.issn.1008-3871.2006.02.016
[20]	山西省食品安全地方标准审评委员会. DBS14/001-2017食品安全地方标准连翘叶[S]. 北京: 中国标准出版社, 2017. Shanxi local food safety standard review committee. DBS14/001-2017 local food safety standard Forsythia leaf [S]. Beijing: China Standards Press, 2017.
[21]	Wang L, Piao X L, Piao X S, et al. Effects of Forsythia suspensa extract on the growth performance, nutrient digestibility, and antioxidant activities in broiler chickens under high ambient temperature[J]. Poult Sci,2008,87:12871294.
[22]	许佳, 张立伟. 青翘的HPLC指纹图谱研究[J]. 食品工程,2013(2):61−64. [Xu J, Zhang L W. Fingerprints of forsythia suspense vahl by HPLC[J]. Food Engineering,2013(2):61−64. doi: 10.3969/j.issn.1673-6044.2013.02.020
[23]	盛楠, 鲁冠杰, 付岳, 等. 连翘酯苷A对感染H9N2-AIV小鼠的炎症基因水平表达的影响[J]. 中国动物保健,2020,22(4):49−50. [Shang N, Lu G J, Fu Y, et al. Effect of forsythioside A on expression of inflammatory genes in mice infected with H9N2-AIV[J]. China Animal Health,2020,22(4):49−50. doi: 10.3969/j.issn.1008-4754.2020.04.039
[24]	Pan C W, Zhou G Y, Chen W L, et al. Protective effect of forsythiaside A on lipopolysaccharide/d-galactosamine-induced liver injury[J]. International Immunopharmacology,2015,26(1):80−85. doi: 10.1016/j.intimp.2015.03.009
[25]	余亮, 江惠丽, 刘豪杰. 连翘酯苷A对溃疡性结肠炎模型大鼠免疫功能的影响[J]. 中国病理生理杂志,2020,36(6):1128−1132, 1139. [Yu L, Jiang H L, Liu H J. Effect of forsythiaside A on immune function in rats with ulcerative colitis[J]. Chinese Journal of Pathophysiology,2020,36(6):1128−1132, 1139. doi: 10.3969/j.issn.1000-4718.2020.06.024
[26]	王菲, 黄毅, 李延森, 等. Keap1/Nrf2/ARE通路相关基因在热诱导的氧化应激小鼠肝脏中的表达[J]. 中国生物化学与分子生物学报,2014,30(3):284−290. [Wang F, Huang Y, Li Y S, et al. Expression of Keap1 /Nrf2 /ARE pathway-related genes in protective response to heat-induced oxidative stress in the liver of mice[J]. Chinese Journal of Biochemistry and Molecular Biology,2014,30(3):284−290.
[27]	李洁蕾, 杨培歌, 冯跃进, 等. L-肉碱对热应激大鼠机体脂质代谢的影响[J]. 动物营养学报,2015,27(9):2849−2855. [Li J L, Yang P G, Feng Y J, et al. Effects of L-carnitine on lipid metabolism of rats under heat stress[J]. Chinese Journal of Animal Nutrition,2015,27(9):2849−2855. doi: 10.3969/j.issn.1006-267x.2015.09.023
[28]	郑灿财, 黄艳玲, 肖芳. 铬源与铬水平对常规饲养肉仔鸡生长性能、免疫器官指数及肉品质的影响[J]. 动物营养学报,2015,27(8):2378−2387. [Zheng C C, Huang Y L, Xiao F. Effects of chromium sources and chromium levels on growth. performance, lmmune organ indexes and meat quality of broilers under normal rearing condition[J]. Chinese Journal of Animal Nutrition,2015,27(8):2378−2387. doi: 10.3969/j.issn.1006-267x.2015.08.009
[29]	张云山, 罗海吉, 吉雁鸿, 等. L-精氨酸对热应激大鼠胸腺和脾脏的影响[J]. 中国公共卫生,2004,20(5):88−89. [Zhang Y S, Lu H J, Ji Y H, et al. Effects of supplementary L-arginine on thymus and spleen in heat- stressed rats[J]. Chinese Journal of Public Health,2004,20(5):88−89.
[30]	张学人. 中药提取物连翘酯苷A对内毒素血症小鼠的免疫调节作用及相关机制研究[J]. 中医药导报,2016,22(21):57−60. [Zhang X R. Effect of forsythiaside A on immune requlation in endotoxemia mice and mechanism of action[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacy,2016,22(21):57−60.
[31]	Dong L L, Yang Z, Su Xi X, et al. Phenylethanoid glycosides from Forsythia suspensa Vahl[J]. Journal of Chinese Pharma-ceutical Sciences,1998,7(2):103−105.
[32]	Boyle M P, Brewer J A, Funatsu M, et al. Acquired deficit of forebrain glucocorticoid receptor produces depression-like changes in adrenal axis regulation and behavior[J]. Proceedings of the National Academy of Sciences of the United States of America,2005,102(2):473−478. doi: 10.1073/pnas.0406458102
[33]	刘法东, 耿瑶, 陶虹, 等. 急性热应激对大鼠血清ACTH及CORT水平的影响[J]. 宁夏医科大学学报,2014,36(10):1079−1081. [Liu F D, Geng Y, Tao H, et al. Effect of acute heat stress in rats on the contents of ACTH and CORT[J]. Journal of Ningxia Medical University,2014,36(10):1079−1081.
[34]	黄帆, 吕秋凤. 热应激诱导的氧化应激对动物肠道组织的损伤[J]. 动物营养学报,2017,29(6):1856−1860. [Huang F, Lyu Q F. Oxidative Stress induced by heat stress: Injury on intestinal tissue of animals[J]. Chinese Journal of Animal Nutrition,2017,29(6):1856−1860. doi: 10.3969/j.issn.1006-267x.2017.06.005
[35]	卫倩, 李萍, 吴桐, 等. 连翘中苯乙醇苷类成分的研究进展[J]. 中国临床药理学杂志,2018,34(20):2481−2485. [Wei Q, Li P, Wu T, et al. Progress in phenylethanoid glycosides from Forsythia suspensa[J]. The Chinese Journal of Clinical Pharmacology,2018,34(20):2481−2485.

Supplements (0)

Cited By

Cited by

Periodical cited type(10)

1.	韩勇. 饲料用连翘叶不同提取物体外抗炎抑菌活性的比较研究. 饲料工业. 2024(07): 86-92 .
2.	滕文龙，吴永娜，王德富，牛颜冰. 连翘叶茶对肝癌细胞增殖和迁移功能的影响及其作用机制. 生物技术通报. 2024(04): 287-296 .
3.	周孝琼，张海燕，郭水灵，王萌萌，陆元安，周华林，王华. 百香果果壳提取物对热应激小鼠的保护作用. 中国兽医杂志. 2024(05): 118-124 .
4.	王中一，张治杰，姚亚乐，陈国雯，马小燕，安志霞，范碧玥，邱山桐，王萌. 连翘叶乙醇提取物对APAP诱导肝损伤小鼠氧化应激及炎症因子的影响. 甘肃农业大学学报. 2024(04): 1-8 .
5.	陈星蕊，汤瑜晨，庄家蝶，黄亚南，钱心悦，张博涛，王梦苒，胡云飞. 连翘不同部位化学成分及药理作用研究进展. 甘肃中医药大学学报. 2024(06): 53-67 .
6.	王学方，陈玲，宁二娟，归荣，王伟，范毅，王学兵，李晓. 连翘叶中7种成分的抗氧化活性研究. 饲料研究. 2023(02): 94-99 .
7.	栗粟，陈红跃，樊艳，王灿，于洋，梁栋，申鹏，张小路. 连翘提取物对实验性自身免疫性甲状腺炎大鼠甲状腺损伤的影响. 中国免疫学杂志. 2023(05): 978-982 .
8.	杨钰昆，杨岚清，梁小祥，王小敏. 连翘叶风味爆珠的制备工艺研究. 食品科技. 2022(01): 286-292 .
9.	范碧玥，王萌，潘阳阳，安志霞，马小燕，叶得河，王桂荣. 连翘叶酶-醇提取物抗药物性肝损伤作用研究. 西北农林科技大学学报(自然科学版). 2022(10): 23-33 .
10.	李欧，刘银，张晓燕，高小康. 十堰地区连翘叶提取物的抑菌和抗氧化活性研究. 饲料研究. 2021(23): 88-93 .