Inactivation Effects and Mechanisms of Plasma-Activated Water against <i>S. typhimurium</i>

Qisen XIANG; Rong ZHANG; Guihong DU; Limin WANG; Aimin JIANG

doi:10.13386/j.issn1002-0306.2020080241

Volume 42 Issue 8

Apr. 2021

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Science and Technology of Food Industry > 2021 > 42(8): 138-143. > DOI: 10.13386/j.issn1002-0306.2020080241

XIANG Qisen, ZHANG Rong, DU Guihong, et al. Inactivation Effects and Mechanisms of Plasma-Activated Water against S. typhimurium [J]. Science and Technology of Food Industry, 2021, 42(8): 138−143. (in Chinese with English abstract). doi: 10.13386/ j.issn1002-0306.2020080241.

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Inactivation Effects and Mechanisms of Plasma-Activated Water against S. typhimurium

Qisen XIANG^{1, 2, 3, 4,},
Rong ZHANG³,
Guihong DU^{2, 4},
Limin WANG⁴,
Aimin JIANG^1, ,

1.
College of Food Science, South China Agricultural University, Guangzhou 510642, China
2.
Henan Doyoo Industrial Co., Ltd., Hebi 456750, China
3.
College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China
4.
Kaifeng Doyoo Industrial Co., Ltd., Kaifeng 475000, China

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Received Date: August 25, 2020
Available Online: January 27, 2021

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Abstract

Abstract

Plasma-activated water (PAW) is an emerging non-thermal sterilization technology, which shows potential applications in food preservation. The present study aimed to elucidate the antibacterial effect and underlying mechanisms of PAW against S. typhimurium cells. PAW obtained by plasma discharge for 30, 60, and 90 s were recorded as PAW30, PAW60, and PAW90, respectively. The results showed that the bactericidal effect of PAW against S. typhimurium cells was gradually enhanced with the increasing plasma discharge time. The population of S. typhimurium was decreased by 4.22 lg CFU/mL after PAW60 treatment for 10 min, which was significantly (P<0.05) lower than that of the control group (7.91 lg CFU/mL). The results of scanning electron microscope (SEM) indicated that the PAW60 treatment resulted in obvious changes in the morphology of S. typhimurium cells. The PAW60 treatment also caused severe disruption of the membrane integrity and permeability of S. typhimurium cells, leading to the significant leakages of cytoplasmic nucleic acids and proteins. After the PAW60 treatment, the extracellular membrane integrity of S. typhimurium cells was destroyed and the intracellular reactive oxygen species levels were also significantly increased. In summary, PAW could effectively inactivate S. typhimurium cells, which might be related to the changes in the structure and membrane permeability of S. typhimurium cells. The results of this study provide theoretical basis for the application of PAW in food sterilization and preservation.
- plasma-activated water,
- S. typhimurium,
- inactivation efficacy,
- mechanisms

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