Simultaneous Determination of Six Functional Components in Chishui Sun Vinegar by RP-HPLC

YANG Sha; XIANG Liping; LING Lei; ZHANG Ji; LU Ye; WANG Lanlan; WANG Ao

doi:10.13386/j.issn1002-0306.2020070313

Volume 42 Issue 3

Jan. 2021

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Science and Technology of Food Industry > 2021 > 42(3): 247-252. > DOI: 10.13386/j.issn1002-0306.2020070313

YANG Sha, XIANG Liping, LING Lei, ZHANG Ji, LU Ye, WANG Lanlan, WANG Ao. Simultaneous Determination of Six Functional Components in Chishui Sun Vinegar by RP-HPLC[J]. Science and Technology of Food Industry, 2021, 42(3): 247-252. DOI: 10.13386/j.issn1002-0306.2020070313

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Simultaneous Determination of Six Functional Components in Chishui Sun Vinegar by RP-HPLC

Zunyi Insitute of Products Quality Insection and Testing, Zunyi 563000, China

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Received Date: July 26, 2020
Available Online: February 02, 2021

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Abstract

Abstract

Objective:To establish a method for determining the contents of six functional components including protocatechuic acid,epicatechin,caffeic acid,vanillic acid,ferulic acid and ligustrazinein in Chishui sun Vinegar by RP-HPLC. Methods:The separation was performed on the Waters xSelect HSS T3(5 μm,4.6 mm×250 mm)column with gradient elution. The mobile phase was 0.1% phosphoric acid water solution and acetonitrile,and the flow rate was set as 0.8 mL/min. The column temperature was kept at 30 ℃. The detection wavelength was set at 285 nm. Results:The linear ranges of protocatechuic acid,epicatechin,caffeic acid,vanillic acid,ferulic acid and ligustrazine were within 10.013~320.416,10.015~320.48,10.012~320.384,10.001~320.032,10.021~320.672,10.072~322.304 μg/mL,respectively. The average recovery rates for six components were within 92.93%~104.53% with RSD less than 3%. The limit of determination was 0.30~1.80 μg/mL and the limit of quantification was 1.02~5.52 μg/mL. Except for caffeic acid,the remaining 5 functional components were detected in the seven sun vinegar samples. The amount of vanillic acid in vinegar was much higher than other components. Conclusion:This method is simple,accurate and suitable for the determination of the protocatechuic acid,epicatechin,caffeic acid,vanillic acid,ferulic acid and ligustrazine in Chishui Sun Vinegar.
- chishui sun vinegar,
- functional components,
- ligustrazine,
- phenolic acids,
- HPLC

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[1]	Samad A,Azlan A,Ismail A. Therapeutic effects of vinegar:A review[J]. Current Opinion in Food Science,2016,8:56-61.
[2]	Yusoff N A,Yam M F,Beh H K,et al. Antidiabetic and antioxidant activities of Nypa fruticans Wurmb. vinegar sample from Malaysia[J]. Asian Pacific Journal Tropical Medicine,2015,8(8):595-605.
[3]	李震宇. 山西老陈醋指纹图谱及功能成分研究[D]. 太原:山西大学,2017.
[4]	包伊凡,沈新春,汪芳. 咖啡酸及其主要衍生物的研究进展及开发前景[J].天然产物研究与开发,2018,30(10):1825-1833.
[5]	Beck J J,Kim J H,Campbell B C,et al. Fungicidal activities of dihydroferulic acid alkyl esteranalogues[J]. Journal of Natural Products,2007,70(5):779-782.
[6]	Anabela B,Maria J S,Manuel S. The activity of ferulic and gallic acids in biofilm prevention and control of pathogenic bacteria[J]. Biofouling,2012,28(7):755-767.
[7]	Kumar S,Prahalathan P,Raja B. Vanillic acid:A potential inhibitor of cardiac and aortic wall remodeling in l-NAME induced hypertension through upregulation of endothelial nitric oxide synthase[J]. Environment Toxicology Pharmacology,2014,38(2):643-652.
[8]	Xiao H H,Gao Q G,Zhang Y,et al. Vanillic acid exerts oestrogen-like activities in osteoblast-like UMR 106 cells through MAP kinase(MEK/ERK)-mediated ER signaling pathway[J].The Journal of Steroid Biochemistry and Molecular Biology,2014,144:382-391.
[9]	王晗. 原儿茶酸对脂肪干细胞体外增殖和迁移的影响[D].大连:大连理工大学,2008.
[10]	吴文华,张晓雷,韩凤梅. 原儿茶酸与拉米夫定体外联合抗HBV药效学研究[J]. 湖北大学学报(自然科学版),2011,33(2):193-196.
[11]	刘厚佳,胡晋红,孙莲娜,等. 原儿茶酸等化合物对HBV DNA转染人癌细胞株的作用[J]. 第二军医大学学报,2001,22(7):661-663.
[12]	贾振宇,孙怡,陈怡飞. 原儿茶酸对阪崎克罗诺肠杆菌的抑制作用[J]. 微生物学通报,2018,45(4):788-796.
[13]	杨美花,石艳,李智聪,等. 原儿茶酸对小鼠黑色素瘤B16细胞酪氨酸酶活力及黑色素生成的抑制效应[J]. 厦门大学学报(自然科学版),2013,52(6):842-845.
[14]	童观珍,付晓萍,杨艳. 表儿茶素的分布及药理活性研究进展[J]. 云南农业大学学报(自然科学),2018,33(2):343-346.
[15]	Xiao Z J,Hou X Y,Liu X,et al. Accelerated green process of tetramethylpyrazine production from glucose and diammonium phosphate[J]. Biotechnology for Biofuels,2014,7(1):106-110.
[16]	Meng W,Xiao D,Wang R. Enhanced production of tetramethylpyrazine in Bacillus licheniformis BL1 by bdhA disruption and 2,3-butanediolsupplementation[J]. World Journal of Microbiology and Biotechnology,2016,32(3):1-7.
[17]	纪凤娣,鲁绯,陶汇源,等. 基于液相色谱的镇江香醋及其相关产品中四甲基吡嗪的分析方法[J]. 食品科学,2019,40(22):293-298.
[18]	朱丽,樊垚,曾泳艇,等. 山西老陈醋中川穹嗪测定及特征分析研究[J].食品科技,2014,39(12):294-298.
[19]	唐杰. 赤水晒醋醋曲品质、微生物多样性及制曲工艺的探究[D]. 重庆:西南大学,2018.
[20]	唐杰,龙晓琴,何银,等. 赤水晒醋的香气成分分析研究[J]. 中国调味品,2017,42(10):135-139.
[21]	刘雪娇,刘超兰,王李,等. 川南晒醋功能特性的分析[J].中国调味品,2018,43(11):141-149.
[22]	Chen T,Gui Q,Shi J J,et al. Analysis of variation of main components during aging process of shanxi aged vinegar[J]. Acetic Acid Bacteria,2013,2(1s):6.
[23]	王戎,廖勤俭,安明哲,等. 白酒中酚酸及酚酸酯检测方法的研究[J]. 酿酒科技,2019(7):110-113.
[24]	李艺. 山西老陈醋指纹图谱及功能成分研究[D]. 太原:山西大学,2017.
[25]	Yao L H. Phenolic acids and abscisic acid in Australian Eucalyptus honeys and their potential for floral authentication[J].Food Chemistry,2004,2(86):169-177.
[26]	Sun C Z,Wang C,Cai Z Z,et al. Determination of flavonoids,phenolic acids and abscisic acid in honeys of different floral origins by HPLC[J]. Food Science,2013,34(10):281-285.
[27]	王戎,廖勤俭,安明哲,等. 白酒中酚酸及酚酸酯检测方法的研究[J]. 酿酒科技,2019(7):110-113.
[28]	李璐,任长忠,王丽丽,等. 燕麦醋中多酚类的含量和组分分析[J]. 中国调味品,2010,35(10):110-112.
[29]	李建龙,刘书亮,彭杨,等. HPLC法同时检测食醋中川芎嗪和阿魏酸的含量[J]. 食品工业科技,2015,36(9):287-291.
[30]	Qing L,Guo Y T,Cai N Z,et al. Antioxidant activities,phenolic profiles,and organic acid contents of fruit vinegar[J]. Antioxidants,2019,8(78):1-12.

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