Synergistic Anti-tumor Effect of Dihydromyricetin and Myricitrin on HepG2 cells

Qiuping CHEN; Xinjian LIN; Shenlan LI; Hesheng LIU; Xiangyang QI

doi:10.13386/j.issn1002-0306.2020070271

Volume 42 Issue 8

Apr. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(8): 333-337. > DOI: 10.13386/j.issn1002-0306.2020070271

CHEN Qiuping, LIN Xinjian, LI Shenlan, et al. Synergistic Anti-tumor Effect of Dihydromyricetin and Myricitrin on HepG2 cells[J]. Science and Technology of Food Industry, 2021, 42(8): 333−337. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306. 2020070271.

Citation:

PDF (1133 KB)

Synergistic Anti-tumor Effect of Dihydromyricetin and Myricitrin on HepG2 cells

College of Biological & Environmental Sciences, Zhejang Wanli University, Ningbo 315100, China

More Information

Received Date: July 22, 2020
Available Online: January 31, 2021

Graphical Abstract

Abstract

Abstract

Objective: To investigate the synergistic anti-tumor effect of Dihydromyricetin (DMY) and Myricitrin (MYT), which were the main active compounds in vine tea. Methods: The HepG2 cells were treated with DMY, MYT and DMY: MYT at the ratios of 1:4, 1:2, 1:1, 2:1, 4:1, 8:1, respectively. The activity of HepG2 cell was measured by MTT assay and fluorescence staining. The synergistic effect of DMY and MYT was analyzed by combination index (CI) and isobole. Results: DMY and MYT could inhibit the proliferation of HepG2 cells with IC₅₀values of 56.46 and 95.01 μmol/L, respectively. When the mixed ratios of the two compounds were 1:4～8:1, their IC₅₀ values on the HepG2 cell proliferation were 40.90～73.00 μmol/L, and the CI values were 0.60～0.86. The mixed ratio of 1:1 showed the strongest synergistic effect, isobole analysis also indicated that there was a synergistic effect between two compounds. Conclusions: The combination of DMY and MYT could effectively promote the activity on inhibiting HepG2 cell proliferation, showing a synergistic effect, which would provide a basis for the further functional research and application of vine tea.
- dihydromyricetin,
- Myricitrin,
- HepG2 cells,
- proliferation inhibition,
- synergism,
- vine tea

FullText(HTML)

References (27)

References

[1]	陈玉琼, 倪德江, 程倩, 等. 藤茶总黄酮及二氢杨梅素降血脂作用研究[J]. 茶叶科学,2007,27(3):221−225, 242. doi: 10.3969/j.issn.1000-369X.2007.03.007
[2]	陈秋平, 王晓艳, 屠熠凡, 等. 藤茶中抗氧化剂阻断N-亚硝胺合成的作用[J]. 核农学报,2014,28(9):1641−1646.
[3]	姚欣, 林静瑜, 倪峰, 等. 藤茶提取物对人前列腺癌LNcap细胞凋亡作用研究[J]. 中国民族民间医药,2015,24(5):26−27.
[4]	Wu S, Liu B, Zhang Q, et al. Dihydromyricetin reduced Bcl-2 expression via p53 in human hepatoma HepG2 cells[J]. Plos One,2013,8(11):e76886. doi: 10.1371/journal.pone.0076886
[5]	周防震, 郑小江, 张晓元. 藤茶活性成分二氢杨梅素对人胚肾细胞和肝细胞增殖的影响[J]. 中成药,2015,37(12):2756−2758.
[6]	龚金炎, 薛薇, 李言郡, 等. 藤茶抑制N-亚硝胺的生成及诱导人前列腺癌PC-3细胞凋亡作用[J]. 中成药,2017,31(10):2160−2163. doi: 10.3969/j.issn.1001-1528.2017.10.037
[7]	肖晓莹, 陈继承, 庞杰. 藤茶的功能性及黄酮类化合物提取研究进展[J]. 热带作物学报,2012,33(8):1522−1527. doi: 10.3969/j.issn.1000-2561.2012.08.036
[8]	沈伟, 孙晓杰, 张秀桥, 等. 大叶蛇葡萄化学成分研究[J]. 时珍国医国药,2010,21(4):866−867. doi: 10.3969/j.issn.1008-0805.2010.04.045
[9]	冯淳, 张妮, 周大颖, 等. HPLC测定显齿蛇葡萄叶中4种黄酮类化合物的含量[J]. 食品工业科技,2018,39(24):246−251.
[10]	苏素娇. 显齿蛇葡萄品质评价及其相关药效学研究[D]. 福州: 福建中医药大学, 2014.
[11]	范莉, 侯小龙, 王文清, 等. 指纹图谱结合一测多评模式在藤茶质量评价中的应用研究[J]. 中草药,2016,47(22):4076−4081.
[12]	徐容容, 张英. 杨梅素和杨梅苷对一些肿瘤细胞的体外抑制及有道凋亡作用研究[J]. 营养学报,2013,35(3):302−303, 306.
[13]	Huang X, Lian T, Guan X, et al. Dihydromyricetin reduces TGF-beta via p53 activation-dependent mechanism in hepatocellular carcinoma HepG2 Cells[J]. Protein & Peptide Letters,2017,24(5):419−424.
[14]	Tomas-Hernandez S, Blanco J, Rojas C, et al. Resveratrol potently counteracts quercetin starvation-induced autophagy and sensitizes HepG2 cancer cells to apoptosis[J]. Molecular Nutrition & Food Research,2018,62(5):1700610.
[15]	曹少谦, 刘亮, 朱孟飞, 等. 乌贼墨多糖提取物的制备及其对B16F10细胞的影响[J]. 核农学报,2017,31(5):906−912. doi: 10.11869/j.issn.100-8551.2017.05.0906
[16]	Liu F, Chen J F, Wang Y, et al. Cytotoxicity of lanostane-type triterpenoids and ergosteroids isolated from Omphalia lapidescens on MDA-MB-231 and HGC-27 cells[J]. Biomedicine & Pharmacotherapy,2019,118:109273.
[17]	Chou T C. Drug combination studies and their synergy quantification using the chou-talalay method[J]. Cancer Research,2010,70(2):440−446. doi: 10.1158/0008-5472.CAN-09-1947
[18]	袁守军. 多药合用药效学协同、相加和拮抗定量计算新方法的建立[J]. 中国药理学与毒理学杂志,2016,30(12):1316−1332. doi: 10.3867/j.issn.1000-3002.2016.12.011
[19]	Tallarida R J. Drug combinations: Tests and analysis with isoboles[J]. Curr Protoc Pharmacol,2016,72(9):191−199.
[20]	皮卓, 李琳娜, 杨德宣, 等. Loewe等效线法计算盐霉素和索拉非尼合用抑制人肺腺癌细胞A549生长的协同相加和拮抗浓度范围[J]. 解放军药学学报,2016,32(4):293−298.
[21]	侯小龙, 王文清, 施春阳, 等. 二氢杨梅素药理作用研究进展[J]. 中草药,2015,46(4):603−609.
[22]	Zhu H, Luo P, Fu Y, et al. Dihydromyricetin prevents cardiotoxicity and enhances anticancer activity induced by adriamycin[J]. Oncotarget,2015,6(5):3254−3267. doi: 10.18632/oncotarget.2410
[23]	周海云. 二氢杨梅素药理及药物相互作用研究进展[J]. 中草药,2018,49(14):3411−3418. doi: 10.7501/j.issn.0253-2670.2018.14.030
[24]	Zhou D Z, Sun H Y, Yue J Q, et al. Dihydromyricetin induces apoptosis and cytoprotective autophagy through ROS-NF-kappaB signalling in human melanoma cells[J]. Free Radical Research,2017,51(5):517−528. doi: 10.1080/10715762.2017.1328552
[25]	苏敬泽, 林谦, 农一兵. 黄芪组分配伍对血管紧张素Ⅱ致肥大心肌细胞模型线粒体活力的影响[J]. 北京中医药,2007,26(11):742−744.
[26]	江湧, 方永奇, 魏刚, 等. 优选细辛醚组合对β-淀粉样蛋白诱导PC12细胞损伤的保护作用[J]. 湖北中医药大学学报,2007,9(1):15−17. doi: 10.3969/j.issn.1008-987X.2007.01.009
[27]	Brahmbhatt M, Gundala S R, Asif G, et al. Ginger phytochemicals exhibit synergy to inhibit prostate cancer cell proliferation[J]. Nutrition and Cancer,2013,65(2):263−272. doi: 10.1080/01635581.2013.749925

Supplements (0)

Cited By

Cited by

Periodical cited type(5)

1.	佘文海，廖威龙，赵永强，董浩. 磷酸盐的持水机理及其在鱼糜加工中的应用. 轻工科技. 2025(01): 51-54 .
2.	郭兵兵，胡澳，陈梦婷，吴文锦，李玟君，关叶霞，熊光权，石柳，陈胜，郭晓嘉，汪超，汪兰，李玮. 魔芋葡甘聚糖对鱼糜抗冻机理和品质特性的研究进展. 现代食品科技. 2025(01): 331-342 .
3.	崔悦，刘锞琳，许龙，祝超智，马长明，余小领. 抗冻剂提高肉糜类制品冻融稳定性的研究进展及展望. 河南科技学院学报(自然科学版). 2024(02): 33-40 .
4.	郭雨晨，赵源，李振坤，石林凡，刘淑集，刘智禹，翁武银. 海水鱼抗冻剂中复合磷酸盐的优化和应用研究. 渔业研究. 2024(05): 458-467 .
5.	王哲，王晓雯，张新笑，王灵娟，秦晓娟，徐为民，王道营，邹烨. 肉制品食品抗冻剂的作用机制及其应用研究进展. 肉类研究. 2023(11): 50-55 .