Optimization of Ultrasonic-assisted Extraction Conditions and Physico-chemical Analysis of Mycelia Polysaccharides from <i>Paecilomyces hepiali</i>

Zhongwei WU; Zhaohui ZHANG; Doudou LENG; Guang ZHANG; Linlin SONG; Bin WANG; Li MENG; Liyou QIU

doi:10.13386/j.issn1002-0306.2020070258

Volume 42 Issue 10

May 2021

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Science and Technology of Food Industry > 2021 > 42(10): 175-182. > DOI: 10.13386/j.issn1002-0306.2020070258

WU Zhongwei, ZHANG Zhaohui, LENG Doudou, et al. Optimization of Ultrasonic-assisted Extraction Conditions and Physico-chemical Analysis of Mycelia Polysaccharides from Paecilomyces hepiali [J]. Science and Technology of Food Industry, 2021, 42(10): 175−182. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020070258.

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Optimization of Ultrasonic-assisted Extraction Conditions and Physico-chemical Analysis of Mycelia Polysaccharides from Paecilomyces hepiali

1.
Crop Science Postdoctoral Research Centre, Henan Agricultural University, Zhengzhou 450046, China
2.
Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang 453003, China
3.
Collaborative Innovation Center of Mordern Biological Breeding, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China

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Received Date: July 21, 2020
Available Online: March 14, 2021

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Abstract

Abstract

PHMPs extraction conditions were optimized by ultrasonic-assisted method from the power of the P. hepiali fermentation mycelium. Then the phenol sulfuric method, Coomassie brilliant blue staining method, Meta-Hydroxydiphenyl, Fourier transform infrared spectroscopy (FT-IR), high performance gel permeation chromatography (HPGPC) and 1-phenyl-3-methyl-5-pyrazolone (PMP) pre-column derivatization method were applied to analyze chemical composition, molecular weight distribution and monosaccharide kinds. Results showed the optimal extraction conditions with an extraction yield of 13.10% ± 0.22% for PHMPs were determined as follows: Extraction temperature 81 ℃, extraction time 36 min, ultrasound power 297 W and ratio of water to raw material 44 mL/g. Furthermore, the chemical composition analysis revealed that the contents of neutral sugar, protein, uronic acid, phosphate were 78.48% ± 2.08%, 9.82% ± 0.09%, 3.76% ± 0.11%, 2.23% ± 0.09%, respectively. The FT-IR spectra showed the several characteristic functional groups absorption of carbohydrate existed in PHMPs. High performance gel permeation chromatography inferred that the PHMPs was composed of two main peaks of 638 and 575 kDa, with a wide molecular weight range of 5.6~285 kDa polysaccharide fractions. The monosaccharide composition tests indicated that there were the main monosaccharides of mannose, glucose and galactose in molar ratios of 42.32:27.58:21.14 and a small amount of ribose, rhamnose, glucuronic acid, galacturonic acid, arabinose in molar ratios of 0.42:2.89:2.25:1.16:2.24 in PHEMPs.
- Paecilomyces hepiali,
- ultrasonic-assisted extraction,
- mycelia polysaccharides,
- physico-chemical analysis

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