Chemical Constituents and Antioxidant Activities in Vitro of Ganoderma leucocontextum

To study the main chemical components and antioxidant activity of Ganoderma leucocontextum, Ganoderma lucidum and Ganoderma sinense were used as control, a variety of analysis methods were used to analyze the nutritional components, polysaccharides, triterpenes, total saponins, ganoderic acid A, amino acids, and trace elements of G. leucocontextum. The scavenging activities of 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) free radical and hydroxyl radical (·OH) of G. leucocontextum extracts from different varieties were investigated. The results showed that the crude protein content and total amino acid content of G. leucocontextum were 18.02%～19.02%, 113.61～163.51 mg/g, which were significantly higher than G. lucidum and G. sinense. The main activity contents of polysaccharides, triterpenoids and ganoderma acid A in G. leucocontextum were 0.99%～1.42%, 1.22%～1.40%, 0.069%～0.084%, respectively, which were significantly higher than in G. lucidum and G. sinense. The content of total amino acids in G. leucocontextum ranged from 113.61 mg/g to 163.51 mg/g, and the contents of glutamic acid and aspartic acid were high. IC₅₀ value of methanol extract from G. leucocontextum on DPPH and hydroxyl free radical were 0.13～0.19 and 0.54～0.89 mg/mL, respectively, showing a good dose-response relationship, and its antioxidant activity was higher than that of G. sinense. This study provides a theoretical basis for the comprehensive development of G. leucocontextum.