ZHAO Lijun, TIAN Xue, ZHONG Wei, et al. Analysis of Fungal Contamination Risk in Pre-packaged Dried Soybean Curd Processing Line Based on High-throughput Sequencing Technology[J]. Science and Technology of Food Industry, 2021, 42(9): 207−213. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020070037.
Citation: ZHAO Lijun, TIAN Xue, ZHONG Wei, et al. Analysis of Fungal Contamination Risk in Pre-packaged Dried Soybean Curd Processing Line Based on High-throughput Sequencing Technology[J]. Science and Technology of Food Industry, 2021, 42(9): 207−213. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020070037.

Analysis of Fungal Contamination Risk in Pre-packaged Dried Soybean Curd Processing Line Based on High-throughput Sequencing Technology

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  • Received Date: July 05, 2020
  • Available Online: March 15, 2021
  • In this study, the fungal contamination in pre-packaged dried soybean curd processing line were analyzed by high-throughput sequencing technology, in combination with culture methods. The contact surface and semi-finished products of food had obvious fungal contamination, Kodamaea, Candida, Trichosporon, Alternaria and Aspergillus were detected to be the predominant contaminating fungal. Boiling and terminal sterilization process efficiently reduce the viable microbial count in soybean curd product. Food contact surfaces showed higher community diversity and abundance than semi-finished products. Conveying tanks, the tofu-covering cloth, the alkaline denaturation tank inner wall, baking inner wall and packaging hopper inner wall were high-risk contact surfaces. Fungi were not the main cause of shelf life spoilage but might induce mycotoxin residues risk.
  • [1]
    卫祥云. 豆制品行业现状及其安全问题的思考[J]. 食品工业科技,2011,32(1):54−55.
    [2]
    徐玉环, 吴月芳. 2019大豆食品重点加工企业调研报告[J]. 大豆科技,2019(6):37−40.
    [3]
    励建荣. 中国传统豆制品及其工业化对策[J]. 中国粮油学报,2005(1):41−44. doi: 10.3321/j.issn:1003-0174.2005.01.011
    [4]
    Ribeiro T T, Costa G, Costa M D. Microbial contamination in industrial tofu[J]. Ciência Rural,2017,47(3):1−6.
    [5]
    管立军, 程永强, 李里特. 豆腐保质期研究进展[J]. 食品工业科技,2008(11):269−272.
    [6]
    Lee D Y, Kwon K H, Chai C, et al. Microbial contamination of tofu in Korea and growth characteristics of Bacillus cereus isolates in tofu[J]. LWT,2017,78:63−69. doi: 10.1016/j.lwt.2016.11.081
    [7]
    毛佳怡, 秦礼康, 曾海英, 等. 传统卤豆干工艺优化及其对风味物质的影响[J]. 食品工业科技,2019,40(23):137−142,150.
    [8]
    卫拯友, 吴富强, 党永, 等. 洛南豆干主要致腐微生物的分离及生物防腐研究[J]. 陕西农业科学,2013,59(2):42−45. doi: 10.3969/j.issn.0488-5368.2013.02.013
    [9]
    Rossi F, Felis G E, Martinelli A, et al. Microbiological characteristics of fresh tofu produced in small industrial scale and identification of specific spoiling microorganisms (SSO)[J]. LWT,2016,70:280−285. doi: 10.1016/j.lwt.2016.02.057
    [10]
    曾齐. 不同生长期大豆根际真菌群落结构动态变化研究[D]. 哈尔滨:哈尔滨师范大学, 2019.
    [11]
    魏思楠, 朱晓峰, 陈立杰, 等. 我国不同地区大豆主栽品种种子携带真菌研究[J]. 大豆科学,2014,33(6):890−895.
    [12]
    陈嵘. 我国部分地区自然发酵豆酱中真菌的多样性和真菌毒素的检测[D]. 沈阳:沈阳农业大学, 2008.
    [13]
    Xu H, Zhang X, Yao W, et al. Characterization of the vaginal microbiome during cytolytic vaginosis using high-throughput sequencing[J]. J Clin Lab Anal,2019,33(1):e22653. doi: 10.1002/jcla.22653
    [14]
    古其会, 吴清平, 张菊梅, 等. 高通量测序技术在饮用水微生物多样性研究中的应用[C]//中国食品科学技术学会. 中国食品科学技术学会第十三届年会论文摘要集. 中国食品科学技术学会: 中国食品科学技术学会, 2016: 466−467.
    [15]
    Liu Y R, Delgado-Baquerizo M, Bi L, et al. Consistent responses of soil microbial taxonomic and functional attributes to mercury pollution across China[J]. Microbiome,2018,6(1):183, 1−12.
    [16]
    Ezeokoli O T, Gupta A K, Mienie C, et al. “PCR-denaturing gradient gel electrophoresis analysis of microbial community in soy-daddawa, a Nigerian fermented soybean (Glycine max (L.) Merr.) condiment.”[J]. Int J Food Microbiol,2016,220:58−62. doi: 10.1016/j.ijfoodmicro.2016.01.003
    [17]
    Xu D, Wang P, Zhang X, et al. “High-throughput sequencing approach to characterize dynamic changes of the fungal and bacterial communities during the production of sufu, a traditional Chinese fermented soybean food.”[J]. Food Microbiol,2020,86:103340. doi: 10.1016/j.fm.2019.103340
    [18]
    陈玉婷, 乌日娜. 传统发酵制品中霉菌的应用研究[J]. 中国酿造,2019,38(7):1−4. doi: 10.11882/j.issn.0254-5071.2019.07.001
    [19]
    葛志文, 方宝庆, 邱伟芬, 等. 储藏稻谷优势霉菌的分离鉴定及特征分析[J]. 粮油食品科技,2019,27(3):72−77.
    [20]
    郑贺云, 姚军, 耿新丽, 等. 食品添加剂对甜瓜采后病原真菌抑制作用研究[J]. 中国食品添加剂,2019,30(10):117−121.
    [21]
    梁明, 陈杰锋, 冼燕萍, 等. QuEChERS-UPLC-MS/MS测定焙烤食品中4种链格孢菌毒素[J]. 现代食品科技,2019,35(6):280−286.
    [22]
    Kumar A, Kumari M, Swarupa P, et al. Characterization of pH dependent growth response of agriculturally important microbes for development of plant growth promoting bacterial consortium[J]. J Pure Appl Microbio,2019,13(2):1053−1061. doi: 10.22207/JPAM.13.2.43
    [23]
    赵明秋, 沈海燕, 潘文, 等. 念珠菌病的流行病学新动态及防控措施[J]. 畜牧兽医科技信息,2011(5):1−4. doi: 10.3969/J.ISSN.1671-6027.2011.05.001
    [24]
    姜冬梅, 王瑶, 姜楠, 等. 农产品及其制品中交链孢酚和交链孢酚单甲醚研究进展[J]. 食品科学,2017,38(21):287−293. doi: 10.7506/spkx1002-6630-201721045
    [25]
    赖红琳, 李强. 真菌毒素与肺部疾病[J]. 中华结核和呼吸杂志,2005(10):60−62.
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