ZHONG Shengnan, YU Xiaobo, HU Ye, NONG Xiang. The Antioxidant Activity and Monosaccharide Composition Analysis of the Polysaccharide from Scorias spongiosa[J]. Science and Technology of Food Industry, 2021, 42(6): 62-66. DOI: 10.13386/j.issn1002-0306.2020060281
Citation: ZHONG Shengnan, YU Xiaobo, HU Ye, NONG Xiang. The Antioxidant Activity and Monosaccharide Composition Analysis of the Polysaccharide from Scorias spongiosa[J]. Science and Technology of Food Industry, 2021, 42(6): 62-66. DOI: 10.13386/j.issn1002-0306.2020060281

The Antioxidant Activity and Monosaccharide Composition Analysis of the Polysaccharide from Scorias spongiosa

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  • Received Date: June 23, 2020
  • Available Online: March 15, 2021
  • The monosaccharide composition and antioxidant activity of Scorias spongiosa polysaccharide(SSP) were analyzed. The aim of this study was to investigate the polysaccharide contents and antioxidant activities of Scorias spongiosa extract. The SSP obtained through ultrasound-assisted extraction was characterized, and its monosaccharide composition was studied by high-performance liquid chromatography. The antioxidant capacity was evaluated by 1, 1-diphenyl-2-picryl-hydrazyl(DPPH), 2, 2'-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) (ABTS+·), and ·OH.The polysaccharide extraction rate of Scorias spongiosa was 9.61%. The total sugar content was 70.92±1.530 g/100 g, which was mainly composed of glucose, mannose, and galactose in the molar ratio of 1.35:212:1. SSP had a relatively high antioxidant activity as revealed by scavenging ability tests on DPPH·, ABTS+·, and ·OH.Antioxidant assays showed the enhanced potency of SSP with its half maximal inhibitory concentration values of(0.796±0.002), (0.923±0.012), and(1.993±0.026) mg/mL for DPPH·, ABTS+·, and ·OH, respectively. This investigation shows that polysaccharide extracts obtained from Scorias spongiosa might be used as bioactive natural sources in the food, and cosmetic industries. Furthermore, we could further study the other biological activities of the polysaccharides.
  • [1]
    贺新生,刘超洋,林琦,等. 竹类煤烟病大型病原菌-海绵胶煤炱菌的分子鉴定[J].福建林学院学报,2011,31(4):363-367.
    [2]
    张协光,孙天宇,肖伟敏,等. HPLC法同时测定植物样品中3种甾醇含量[J].食品工业,2019,40(1):327-330.
    [3]
    冯望,吴颖,尤雅,姚珂,等. 海绵胶煤炱菌酶活性与多糖抗氧化活性的研究[J].食品与发酵科技,2013,49(5):34-37.
    [4]
    Wu Y,Li T X,Jia X W,et al. Effect of Tween 80 and chloroform on the secretion,structure and cytotoxic activities of exopolysaccharides from Scorias spongiosa[J]. Journal of Biologically Active Products from Nature,2018,8(5):312-318.
    [5]
    袁小红,张春杰,刘霞,等. 6种高等真菌醇提物对植物病原菌的抗菌活性研究[J]. 安徽农业科学,2013,41(12):5289-5290

    ,5294.
    [6]
    袁小红,张春杰,郑仁林,等. 六种高等真菌醇提物的抗肿瘤活性筛选[J]. 西南科技大学学报,2013,28(3):95-97.
    [7]
    Zhong S N,Yu H,Nong X,et al. A mini-review of distribution,growth environment and nutrient composition from Scorias spongiosa(Bamboo Bird's Nest)[J]. IOP Conf. Series:Materials Science and Engineering,2020,782:042040.
    [8]
    梁雪,倪秀珍,汉丽萍. 食药用真菌多糖生物活性的研究进展[J]. 长春师范大学学报,2018,37(10):82-85.
    [9]
    徐盼菊. 黑虎掌菌子实体粗多糖制备及其生物活性研究[D]. 长春:吉林大学,2017.
    [10]
    Dubois M,Gilles K A,Hamilton J K,et al. Colorimetric method for determination of sugars and related substances[J]. Analytical Chemistry,1956,28(3):350-356.
    [11]
    戴军,朱松,汤坚,等. PMP柱前衍生高效液相色谱法分析杜氏盐藻多糖的单糖组成[J].分析测试学报,2007,26(2):206-210.
    [12]
    Chen Z,Zhang W,Tang X,et al. Extraction and characterization of polysaccharides from Semen Cassiae by microwave-assisted aqueous two-phase extraction coupled with spectroscopy and HPLC[J]. Carbohydrate Polymers,2016:263-270.
    [13]
    罗懿洋,任道远,陈丽芳,等. 杏鲍菇多糖的单糖组成分析及其抗氧化活性研究[J]. 食品工业科技,2015,36(8):158-161

    ,166.
    [14]
    Sakina R,Kandikattu K,Ilaiyaraja N,et al. Antioxidant,biomolecule oxidation protective activities of nardostachysjatamansi DC and its phytochemical analysis by RP-HPLC and GC-MS[J]. Antioxidants,2015,4(1):185-203.
    [15]
    Abbey S,Amin S,Hongxia Z,et al. Antioxidant activities and caffeic acid content in New Zealand Asparagus(Asparagus officinalis)roots extracts[J]. Antioxidants,2018,7(52):1-13.
    [16]
    Jiang Y Y,Wang L,Zhang L,T.et al. Optimization of extraction and antioxidant activity of polysaccharides from Salvia miltiorrhiza bunge residue[J]. International Journal of Biological Macromolecules,2015,79:533-541,
    [17]
    Zhang J X,Wen C T,Gu J Y,et al. Effects of subcritical water extraction microenvironment on the structure and biological activities of polysaccharides from Lentinus edodes[J]. International Journal of Biological Macromolecules,2019,123:1002-1011.
    [18]
    Yan J K,Ding Z C,Gao X L,et al. Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions[J]. Carbohydrate Polymers,2018,193:373-82.
    [19]
    Aytursun A,Parhat R,Paiheerding M,et al. Effects of different extraction techniques on physicochemical properties andbiological activities of polysaccharides from Fritillaria pallidiflora Schrenk[J]. Process Biochemistry,2019,83:189-197.
    [20]
    Kang Q Z,Chen S S,Li S F,et al. Comparison on characterization and antioxidant activity of polysaccharides from Ganoderma lucidum by ultrasound and conventional extraction[J].International Journal of Biological Macromolecules,2019,124:1137-1144.
    [21]
    闫征,郝利民,张黎明,等. 3种不同产地灵芝子实体主要化学成分比较[J]. 食品科学,2019,40(6):240-246.
    [22]
    赵诗雨,赵婷婷,梁锐,等. 三个不同产地香菇多糖的理化性质及生物活性研究[J/OL].菌物学报:1-8[2020-08-09

    ]. https://doi.org/10.13346/j.mycosystema.200030.
    [23]
    王家辉,刘杉杉,张鑫,等. 牛蒡不同部位多糖的抗氧化与抗凝血活性研究[J]. 食品工业科技,2020,41(6):305-310.
    [24]
    Chen Y F,Jiang W W,Zhang S Q,et al. Antioxidant activity and characterization of one new polysaccharide obtained from perigord truffle(Tuber huidongense)[J]. Evidence-Based Complementary and Alternative Medicine,2016,(1):1-7.
    [25]
    Ebru D,Fatih Ç,Gülsen T,et al. Structural characterization and determination of biological activities for different polysaccharides extracted from tree mushroom species[J]. Journal of Food Biochemistry,2019,43(9).
    [26]
    Hu Y N,Sung T Z,Chou C H,et al. Characterization and antioxidant activities of yellow strain Flammulina velutipes(Jinhua Mushroom)polysaccharides and their effects on ROS content in L929 Cell[J]. Antioxidant,2019,8(8):298.
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