Extraction of Total Alkaloids from <i>Suaeda salsa</i> and Its Antioxidant Activity in Mice Fed with High-fat Diet

Xiaoyang LI; Guanying HAN; Song YAN; Yandan ZHAO; Bin GUO; Zan CUI

doi:10.13386/j.issn1002-0306.2020060194

Volume 42 Issue 7

Mar. 2021

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Science and Technology of Food Industry > 2021 > 42(7): 188-194. > DOI: 10.13386/j.issn1002-0306.2020060194

LI Xiaoyang, HAN Guanying, YAN Song, et al. Extraction of Total Alkaloids from Suaeda salsa and Its Antioxidant Activity in Mice Fed with High-fat Diet[J]. Science and Technology of Food Industry, 2021, 42(7): 188−194. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060194.

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Extraction of Total Alkaloids from Suaeda salsa and Its Antioxidant Activity in Mice Fed with High-fat Diet

1.
School of Pharmaceutical Sciences, Jinzhou Medical University, Jinzhou 121000, China
2.
The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, China

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Received Date: June 15, 2020
Available Online: January 27, 2021

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Abstract

Abstract

Objective: To optimize the extraction process of total alkaloids from Suaeda salsa L. and study the antioxidant activity of total alkaloids from Suaeda salsa on high-fat diet in mice. Methods: The yield of total alkaloids from Suaeda salsa was used as the index, and the orthogonal analysis was carried out on the basis of single factor experiment. The total alkaloids of Suaeda salsa were gavaged to high-fat diet mice, and the aging indexes of mice were monitored regularly. Results: The optimum extraction conditions were as follows: Ultrasonic time 20 min, ultrasonic temperature 45 ℃, ethanol concentration 85%, solid-liquid ratio 1:10. Under these conditions, the yield of total alkaloids was (0.65 ± 0.02) mg/g. The antioxidant activity test showed that the low, medium and high dose groups of Suaeda salsa total alkaloids could significantly reduce the content of MDA in the liver of mice (P<0.05), and the low(2 mg/kg), medium(4 mg/kg) and high dose(8 mg/kg) groups of total alkaloids of Suaeda salsa could also significantly increase cat and GSH-Px in liver (P<0.05). Conclusion:The optimized process has good feasibility. Suaeda salsa alkaloids can be used as the basis for the study of the total antioxidant activity of Suaeda salsa.
- Suaeda salsa,
- total alkaloids,
- orthogonal experiment,
- extraction process optimization,
- MDA,
- CAT,
- GSH-Px,
- antioxidant activity

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