JING Jinjin, ZHANG Ruoyu, DU Xue, DU Lidong, LIU Zhaoli, LIN Bingjie, ZHANG Fengxiang, XUE Peng. Synthesis and Synergistic Antibacterial Activity of Complexes of Quinoa Saponins with Silver Nanoparticles[J]. Science and Technology of Food Industry, 2021, 42(6): 50-55,61. DOI: 10.13386/j.issn1002-0306.2020060176
Citation: JING Jinjin, ZHANG Ruoyu, DU Xue, DU Lidong, LIU Zhaoli, LIN Bingjie, ZHANG Fengxiang, XUE Peng. Synthesis and Synergistic Antibacterial Activity of Complexes of Quinoa Saponins with Silver Nanoparticles[J]. Science and Technology of Food Industry, 2021, 42(6): 50-55,61. DOI: 10.13386/j.issn1002-0306.2020060176

Synthesis and Synergistic Antibacterial Activity of Complexes of Quinoa Saponins with Silver Nanoparticles

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  • Received Date: June 14, 2020
  • Available Online: March 15, 2021
  • Quinoa saponins and silver nitrateas raw materials, the complexes of quinoa saponins with nano-silver were prepared by biological reduction method, and the antibacterial activity of the complexes was studied. The complexes of quinoa saponins and silver nanoparticles were characterized and analyzed by ultraviolet-visible spectrophotometer(UV-vis spectrophotometer) and transmission electron microscope(TEM). The synergistic antibacterial activity of quinoa saponins and silver nanoparticles were studied by bacteriostatic circle experiment, minimal inhibitory concentration(MIC) experiment, minimum bactericidal concentration(MBC) experiment and so on. The results showed that the complex of quinoa saponins with silver nanoparticles was spherical, and the diameter of the particles was mostly concentrated around 10 nm, with good dispersity. Characteristic absorption peaks appear at 420 nm. The complex of quinoa saponins and nano-silver preform had good inhibitory effect and synergistic inhibitory effect on foodborne microorganisms, and had good inhibitory effect on Staphylococcus epidermidis and Staphylococcus aureus, the bacteriostatic diameter were(11.33±0.58) and(10.67±1.15) mm, respectively. The low toxicity to normal gastric mucosal epithelial cell(GES) and gastric cancer cell(SGC). As a biological reducing agent, quinoa saponins enhanced the solubility and dispersion of silver nanoparticles, retained the broad-spectrum antibacterial properties of silver nanoparticles, and cooperated with each other to enhance their antibacterial properties.
  • [1]
    陈婧雯,张芯.纳米银的制备方法和应用现状[J].山东化工,2019;48(2):58-60

    ,63.
    [2]
    孙彩华,陈水英,余朝辉.大黄提取物制备纳米银抑菌材料形态及抑菌性能研究[J].中华中医药学刊,2017(35):1070-1073.
    [3]
    张元新,葛雅琨.抗菌肽KS26基因的串联及在毕赤酵母中的表达[J]. 吉林化工学院学报,2014(31):14-17.
    [4]
    洪志伟,孟令宇,葛雅琨,等.槲皮素对大肠杆菌和金黄色葡萄球菌的体外抑菌作用的研究[J]. 吉林化工学院学报,2017(34):38-41.
    [5]
    胡滨,刘国军,刘素花,等.抗菌涂料中无机抗菌剂的研究进展[J].现代涂料与涂装,2009,12(1):18-21.
    [6]
    郭春兰,邓红艳,屈红玲.不同银敷料在慢性伤口治疗中应用效果的对比研究[J].护理研究,2015(29):1170-1175.
    [7]
    Maryam A,Sakineh Mesgar Shahroodi. Effects of silver nanoparticles on the thermal properties of polyethylene matrix nanocomposites[J]. Therm Anal Calori,2017,28(2):1117-24.
    [8]
    Tobaldi D M,Piccirillo C,Pullar R C,et al. Silver-modified nano-titania as an antibacterial agent and photocatalyst[J]. The Journal of Physical Chemistry C,2014,118:4751-66.
    [9]
    高柯玄,庙荣荣,何亮. 金属有机配合物的合成及其在催化领域中的应用研究进展[J].广东化工,2018(45):73-75.
    [10]
    李波,孙天颖,于鑫洋.苜蓿皂苷的抑菌活性和抗氧化特性的研究[J].食品研究与开发,2013,34(2):1-2

    ,33.
    [11]
    安继红,吕维玲,张永州,等.人参皂苷对金黄色葡萄球菌生物被膜形成的影响[J].山东大学学报(医学版),2018,56(7):28-32.
    [12]
    Mandal P,Sinha Babu S P,Mandal N C. Antimicrobial activity of saponins from Acacia auriculiformis[J]. Fitoterapia,2005,76(5):462-465.
    [13]
    孟庆然,李立博,王晓闻.酸浆宿萼总皂苷体外抑菌效果研究[J].食品学,2013,34(19):84-87.
    [14]
    胡一晨,赵钢,秦培友,等.藜麦活性成分研究进展[J].作物学报,2018,44(11):1579-91.
    [15]
    Kuljanabhagavad T,Thongphasuk P,Chamulitrat W,et al. Triterpene saponins from Chenopodium quinoa Willd[J]. Phytochemistry,2008,69:1919-26.
    [16]
    Yao Y,Yang X,Shi Z,et al. Anti-inflammatory activity of saponins from quinoa(Chenopodium quinoa Willd.)seeds in lipopolysaccharide-stimulated RAW 264.7 macrophages cells[J].Food Sci,2014,79:1018-1023.
    [17]
    赵雷,李晓娜,史龙龙,等. 藜麦麸皮营养成分测定及其油脂的抗氧化活性研究[J]. 现代食品科技,2019,35(11):157,205

    -211.
    [18]
    陈满堂.枸杞水提物制备纳米银及其应用研究[D].武汉:华中科技大学,2016.
    [19]
    徐雅心,叶赐能,方歆倩,等. 植物提取法制备纳米银及其在个人洗护用品中的应用[J]. 中国洗涤用品工业,2019(4):76-81.
    [20]
    薛海燕,张颖,张宝艳,等.安石榴苷还原壳聚糖/纳米银溶胶制备表征及其抑菌性能[J].农业工程学报,2018,34(4):306-314.
    [21]
    赵雷,丁葵英,郑星,等.藜麦麸皮不同极性部位的抑菌及酪氨酸酶抑制活性研究[J].食品工业科技,2019,40:82-88,94.
    [22]
    Dong S X,Yang X S,Zhao L,et al. Antibacterial activity and mechanism of action saponins from Chenopodium quinoa Willd. husks against foodborne pathogenic bacteria[J]. Industrial Crops and Products,2020.https://doi.org/10.1016/j.indcrop.2020.112350.
    [23]
    刘朗.高价银配合物的合成、表征及抗菌性能研究[D]. 南京:南京航空航天大学,2017.
    [24]
    Veerasamy R,Xin T Z,Gunasagaran S,et al. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities[J]. Journal of Saudi Chemical Society,2011,15:113-120.
    [25]
    Park S Y,Park J H,Kim H S,et al. Systems-level mechanisms of action of Panax ginseng:A network pharmacological approach[J]. Ginseng Res,2018,42:98-106.
    [26]
    Cao Y,Zheng R,Ji X,et al. Syntheses and characterization of nearly monodispersed,size-tunable silver nanoparticles over a wide size range of 7~200 nm by tannic acid reduction[J]. Langmuir,2014,30:3876-3882.
    [27]
    郭春兰,席祖洋,邓红艳,等.纳米银敷料用于体表慢性难愈合伤口的效果及安全性评价[J].广东医学,2016(37):3477-3480.
    [28]
    Nakkala J R,Mata R,Raja K,Khub Chandra V,Sadras S R. Green synthesized silver nanoparticles:Catalytic dye degradation,in vitro anticancer activity and in vivo toxicity in rats[J]. Mater Sci Eng C Mater Biol Appl,2018,91:372-381.
    [29]
    郭春兰,席祖洋,邓红艳,等.纳米银敷料用于体表慢性难愈合伤口的效果及安全性评价[J].广东医学,2016(37):3477-3480.
    [30]
    Sun W,Qu D,Ma Y,et al. Enhanced stability and antibacterial efficacy of a traditional Chinese medicine-mediated silver nanoparticle delivery system[J]. Int J Nanomedicine,2014,9:5491-502.
    [31]
    徐锐,缪宏超,王伟,等.仙人掌还原纳米银溶液的制备及其抑菌效果研究[J].纺织导报,2010(8):97-99.
    [32]
    方蕾,赵晓丽,王珺瑜,等.具有不同粒径和相同表面结构纳米银颗粒的制备及表征[J].环境科学研究,2019(32):866-874.
    [33]
    Triterpenes. Researchers from kyung hee university report recent findings in triterpenes(use of gold nanoparticle fertilizer enhances the ginsenoside contents and anti-inflammatory effects of red ginseng)[J]. Biotech Week,2017.
    [34]
    Singh P,Kim Y J,Yang D C. A strategic approach for rapid synthesis of gold and silver nanoparticles by Panax ginseng leaves[J]. Artif Cells Nanomed Biotechnol,2016,44(8):1949-1957.
    [35]
    Ravichandran Veerasamy,Tiah,et al. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities[J]. Journal of Saudi Chemical Society,2011,15(2):113-120.
    [36]
    Singh Priyanka,Kim Yeon Ju,Wang Chao,etal. Biogenic silver and gold nanoparticles synthesized using red ginseng root extract,and their applications[J]. Artificial Cells,Nanomedicine,and Biotechnology,2016,44(3):1-6.
    [37]
    强舒悦,吴小玲,姚平,等.茶多酚纳米银的绿色制备及其工艺优化[J].轻工科技,2018,34(7):30-31

    ,40.
    [38]
    曹雪玲,陆书来,张东杰,等.纳米银作为抗菌剂的抗菌性能研究[J].吉林化工学院学报,2017(34):30-34.

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