Determination of Arsenic Species in Dried Edible Fungi by HPLC-ICP-MS Combined with Microwave Acid Extraction Analysis

In this paper, microwave acid extraction and HPLC-ICP-MS were combined to establish a simple and efficient method for the determination of arsenic speciation in dried edible fungi. By comparing the extraction methods and optimizing the extraction conditions, the precision, detection limit, accuracy and recovery of the method were verified. The results showed that the microwave-acid extraction method not only satisfied the extraction efficiency, but also ensured that the forms of arsenic did not change. The separation of (As (III), As (V), MMA, DMA, ASB, ASC was realized within 10 minutes. The linear relationship was good (r > 0.9990), RSD%<10.0%. The quantitative limits were 0.009, 0.012, 0.013, 0.010, 0.011 and 0.010 mg/kg, respectively. The recoveries of morels and black truffle were 83.6%~105.5%. The accuracy of the method was verified by two kinds of standard materials. The method had the advantages of simple pretreatment, good separation effect, good precision and high accuracy, and was suitable for the analysis and determination of arsenic species in dried edible fungi, and it would provide a new method for the determination of inorganic arsenic in edible fungi and basic data for the determination of inorganic arsenic limit in edible fungi.