Separation and Purification of Ellagic Acid from <i>Rosa setate x Rosa rugosa</i> Waste by Macroporous Resin and High-speed Countercurrent Chromatography

WU Mengqi; HUANG Hai; CAI Dihong; XU Zhizhen; XIA Wei; ZHANG Wenqing

doi:10.13386/j.issn1002-0306.2020060147

Volume 42 Issue 5

Feb. 2021

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Science and Technology of Food Industry > 2021 > 42(5): 182-187. > DOI: 10.13386/j.issn1002-0306.2020060147

WU Mengqi, HUANG Hai, CAI Dihong, XU Zhizhen, XIA Wei, ZHANG Wenqing. Separation and Purification of Ellagic Acid from Rosa setate x Rosa rugosa Waste by Macroporous Resin and High-speed Countercurrent Chromatography[J]. Science and Technology of Food Industry, 2021, 42(5): 182-187. DOI: 10.13386/j.issn1002-0306.2020060147

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Separation and Purification of Ellagic Acid from Rosa setate x Rosa rugosa Waste by Macroporous Resin and High-speed Countercurrent Chromatography

School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

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Received Date: June 10, 2020
Available Online: March 02, 2021

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Abstract

Abstract

A purification method of ellagic acid(Ea)from Kushui rose waste and its antioxidant activities were studied. Firstly,the crude alcohol extracts of rose residues were purified by macroporous adsorption resin to enrich the target component. Then,the separation was performed using high-speed count-current chromatography with a two-phase solvent system consisting of n-hexane-ethyl acetate-methanol-water(2:7:3.5:7,v/v/v/v). Ellagic acid with purity of 96.2% was achieved with the flow rate of 3.0 mL·min^-1,the speed of 850 r·min^-1,the system temperature of 30 ℃ and sample loading of 800 mg,upper phase as stationary phase and lower phase as mobile phase. Furthermore,the in-vitro antioxidant test results showed that Ea possessed the strong antioxidant effects on the clearance of DPPH· and ABTS⁺·,with IC₅₀ values of(2.54±0.05)and(9.62±0.17) mg·L^-1,respectively. Meanwhile,the total antioxidant capacity FRAP value was(180.85±3.16) μmol·L^-1·mg^-1. This simple technology obtained the products with high purity,which provided a theoretical basis for the integrated utilization of rose waste biomass.
- Rosa setate x Rosa rugosa,
- flower waste,
- ellagic acid,
- macroporous resin,
- high speed counter-current chromatography

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