Detection of Calcium Peroxide in Flour by Methanobactin-Cu Simulated Peroxidase Method

Jiayu ZHANG; Jiaying XIN; Fengyuan LIU; Chungu XIA; Xikai XU; Guangjiao WANG; Tianyu CUI

doi:10.13386/j.issn1002-0306.2020060120

Volume 42 Issue 9

Apr. 2021

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Science and Technology of Food Industry > 2021 > 42(9): 257-262. > DOI: 10.13386/j.issn1002-0306.2020060120

ZHANG Jiayu, XIN Jiaying, LIU Fengyuan, et al. Detection of Calcium Peroxide in Flour by Methanobactin-Cu Simulated Peroxidase Method[J]. Science and Technology of Food Industry, 2021, 42(9): 257−262. (in Chinese with English abstract). doi: 10.13386/ j.issn1002-0306.2020060120.

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Detection of Calcium Peroxide in Flour by Methanobactin-Cu Simulated Peroxidase Method

1.
Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China
2.
State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China

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Received Date: June 09, 2020
Available Online: February 04, 2021

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Abstract

Abstract

By using the ultraviolet absorption spectrum to detect the change of absorption value of CaO₂ at 288 nm, a fast and accurate method of CaO₂ detecting in flour was established. The results showed that when the Mb-Cu concentration was 3.9×10⁻⁶ mol/L, the detection temperature was 60 ℃, and the detection time was 180 s, the CaO₂ concentration was linear with the change in absorbance at 288 nm, and the linear equation was y=0.00643+0.02117x, R²=0.99281. The detection limit of the method was 1.82×10⁻²mg/L, the average recovery of standard addition was 98.2%～100.6%, and the relative standard deviation RSD was 0.49%～1.32%. This Mb-Cu mimic peroxidase method has high accuracy, fast detection speed, and low detection limit. It can be used in practice for the trace detection of CaO₂ in flour, providing a new strategy for the food detection industry.
- methanobactin-Cu coordination complex (Mb-Cu),
- flour,
- calcium peroxide,
- peroxidase

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References

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