TU Bowen, XUE Yingang, YAO Ping, WAND Nan, LI Junhong, TANG Hongbing, DU Qiang. Virulence Characteristics Application of Vibrio cholera from Different Source by Microbial Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(6): 130-136,150. DOI: 10.13386/j.issn1002-0306.2020060092
Citation: TU Bowen, XUE Yingang, YAO Ping, WAND Nan, LI Junhong, TANG Hongbing, DU Qiang. Virulence Characteristics Application of Vibrio cholera from Different Source by Microbial Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(6): 130-136,150. DOI: 10.13386/j.issn1002-0306.2020060092

Virulence Characteristics Application of Vibrio cholera from Different Source by Microbial Mass Spectrometry

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  • Received Date: June 08, 2020
  • Available Online: March 15, 2021
  • Objective: To analysis the regularities of distribution of Vibrio cholera from food risk monitoring samples and evaluate the merits of VITEK-MS rapid analysis methods in identification of Vibrio cholera. Methods: V. cholera strains were isolated from freshwater products and patients. The VITEK-MS and VITEK-2 identification were operated respectively. The serotype was detected by serum agglutination and immunogold. The virulence genes ctxAB was detected by qPCR. All the virulence genes in different types of strains were analyzed by whole genome sequencing. Results: 17 of the 23 V. cholera strains were non-O1/O139 serotype. 6 strains were detected as O139 V. cholera and ctxAB gene carrier. All the V. cholera strain showed typical mass spectral characteristics based on the fingerprint spectrum. The particular and lacking peaks patterns of O139 V. cholera were representative for Serotype identification. ctxAB, ace and tcp islands genes were harbored in O139 Vibrio cholera, but only auxiliary virulence factor can be detected in non-O1/O139 Vibrio cholera except zot gene. Conclusion: There are many differences between the O139 and non-O1/O139 Vibrio cholera strains in whether virulence gene type or fingerprint spectrum. The thorough application for serotype identification by using microbial mass spectrometry may be a new assistance for cholera surveillance.
  • [1]
    Ali M,Nelson A R,Lopez A L,et al. Updated Global burden of cholera in endemic countries[J]. PLoS Neglected Tropical Diseases,2015,9(6):e0003832. DOI:10.1371/journal. pntd. 0003832.
    [2]
    Ramamurthy T,Das B,Chakraborty S,et al. Diagnostic techniques for rapid detection of Vibrio cholerae O1/O139[J]. Vaccine. 2020,38:A73-A82.
    [3]
    Faruque S M,Chowdhury N,Kamruzzaman M,et al. Genetic diversity and virulence potential of environmental Vibrio cholera population in a cholera-endemic area[J]. Proc Natl Acad Sci U S A,2004,101(7):2123-8.
    [4]
    Gong L,Yu P,Zheng H J,et al. Comparative genomics for non-O1/O139Vibrio cholerae isolates recovered from the Yangtze River Estuary versus V. cholerae representative isolates from serogroup O1[J]. Molecular Genetics and Genomics,2019,294(2):417-430.
    [5]
    Fu S Z,Hao J W,Jin S B,et al. A Human intestinal infection caused by a novel non-O1/O139 Vibrio cholerae genotype and its dissemination along the river[J]. Frontiers in Public Health,2019,7:100.
    [6]
    Baker-Austin C,Trinanes J A,Taylor N G H,et al. Emerging Vibrio risk at high latitudes in response to ocean warming[J]. Nature Climate Change,2012,3(1):73-77.
    [7]
    Frank C,Littman M,Alpers K,et al. Vibrio vulnificus wound infections after contact with the Baltic Sea,Germany[J]. Euro Surveillance,2006,11(8):E060817.1.
    [8]
    Kim E J,Yu H J,Lee J H,et al. Replication of Vibrio cholerae classical CTX phage[J]. Proc Natl Acad Sci USA,2017,114(9):2343-2348.
    [9]
    夏晓滨. 霍乱弧菌生物被膜[J]. 中华微生物学和免疫学杂志,2008,28(4):381-384.
    [10]
    Huq A,Whitehouse C A,Grim C J,et al. Biofilms in water,its role and impact in human disease transmission[J]. Current Opinion in Biotechnology,2008,19(3):244-247.
    [11]
    Chang Y W,Kjær A,Ortega D R,et al. Architecture of the Vibrio cholerae toxin-coregulated Pilus machine revealed by electron cryotomography[J]. Nature Microbiology,2017,2:16269.
    [12]
    Ng D,Harn T,Altindal T,et al. The Vibrio cholerae minor pilin TcpB initiates assembly and retraction of the toxin-coregulated Pilus[J]. PLoS Pathogens,2016,12(12):e1006109.
    [13]
    Rahman M H,Biswas K,Hossain M A,et al. Distribution of genes for virulence and ecological fitness among diverse Vibrio cholerae population in a cholera endemic area:Tracking the evolution of pathogenic strains[J]. DNA and Cell Biology,2008,27(7):347-355.
    [14]
    Chow K H,Ng T K,Yuen K Y,et al. Detection of RTX toxin gene in Vibrio cholerae by PCR[J]. Journal of Clinical Microbiology,2001,39(7):2594-2597.
    [15]
    Satchell K J F.MARTX,multifunctional autoprocessing repeats-in-toxin toxins[J]. Infection and Immunity,2007,75(11):5079-5084.
    [16]
    Erler R,Wichels A,Heinemeyer E A,et al. Vibrio base:A MALDI-TOF MS database for fast identification of Vibrio spp. that are potentially pathogenic in humans[J]. Systematic and Applied Microbiology,2015,38(1):16-25.
    [17]
    Vidal L M R,Venas T M,Gonçalves A R P,et al. Rapid screening of marine bacterial symbionts using MALDI-TOF MS[J]. Archives of Microbiology,2020,202(8):2329-2336.
    [18]
    Kazazić S P,Topić Popović N,Strunjak-Perović I,et al. Matrix-assisted laser desorption/ionization time of flight mass spectrometry identification of Vibrio(Listonella)anguillarum isolated from sea bass and sea bream[J]. PLoS One,2019,14(11):e0225343.
    [19]
    Krishnamurthy T,Ross P L,Rajamani U. Detection of pathogenic and non-pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry[J]. Rapid Communications in Mass Spectrometry:RCM,1996,10(8):883-888.
    [20]
    Bauer A,Rørvik L M. A novel multiplex PCR for the identification of Vibrio parahaemolyticus,Vibrio cholerae and Vibrio vulnificus[J]. Letters in Applied Microbiology,2007,45(4):371-375.
    [21]
    Schirmeister F,Dieckmann R,Bechlars S,et al. Genetic and phenotypic analysis of Vibrio cholerae non-O1,non-O139 isolated from German and Austrian patients[J]. European Journal of Clinical Microbiology & Infectious Diseases,2014,33(5):767-778.
    [22]
    Chatterjee S,Ghosh K,Raychoudhuri A,et al. Incidence,virulence factors,and clonality among clinical strains of non-O1,non-O139Vibrio cholerae isolates from hospitalized diarrheal patients in Kolkata,India[J]. Journal of Clinical Microbiology,2009,47(4):1087-1095.
    [23]
    Pang B,Yan M Y,Cui Z G,et al. Genetic diversity of toxigenic and nontoxigenic Vibrio cholerae serogroups O1 and O139 revealed by array-based comparative genomic hybridization[J]. Journal of Bacteriology,2007,189(13):4837-4849.
    [24]
    Bhattacharya T,Chatterjee S,Maiti D,et al. Molecular analysis of the rstR and orfU genes of the CTX prophages integrated in the small chromosomes of environmental Vibrio cholerae non-O1,non-O139 strains[J]. Environmental Microbiology,2006,8(3):526-634.
    [25]
    Mauritzen J J,Castillo D,Tan D M,et al. Beyond cholera:Characterization of zot-encoding filamentous phages in the marine fish pathogen Vibrio anguillarum[J]. Viruses,2020,12(7):730.
    [26]
    Shanley J,Kanj A,El Zein S,et al. Non-O1,non-O139Vibrio cholerae bacteremia in an urban academic medical center in the United States[J]. IDCases,2019,15:e00527.
    [27]
    Takemura T,Murase K,Maruyama F,et al. Genetic diversity of environmental Vibrio cholerae O1 strains isolated in Northern Vietnam[J]. Infection,Genetics and Evolution,2017,54:146-151.
    [28]
    Xue H,Xu Y,Boucher Y,et al. High frequency of a novel filamentous phage,VCY φ,within an environmental Vibrio cholerae population[J]. Applied and Environmental Microbiology,2012,78(1):28-33.
    [29]
    Faruque S M,Bin Naser,Fujihara K,et al. Genomic sequence and receptor for the Vibrio cholerae phage KSF-1phi:Evolutionary divergence among filamentous vibriophages mediating lateral gene transfer[J]. Journal of Bacteriology,2005,187(12):4095-4103.
    [30]
    Faruque S M,Kamruzzaman M,Asadulghani,et al. CTXphi-independent production of the RS1 satellite phage by Vibrio cholera[J]. Proc Natl Acad Sci U S A,2003,100(3):1280-5.
    [31]
    Paauw A,Trip H,Niemcewicz M,et al. OmpU as a biomarker for rapid discrimination between toxigenic and epidemic Vibrio cholerae O1/O139 and non-epidemic Vibrio cholerae in a modified MALDI-TOF MS assay[J]. BMC Microbiol,2014,14:158.
    [32]
    Chaika S O,Telesmanich N R,Lomov Y M. Mass spectrometry virulence marker Vibrio cholera[J]. Klinicheskaia Laboratornaia Diagnostika,2018,63(7):445-449.
    [33]
    Dieckmann R,Strauch E,Alter T. Rapid identification and characterization of Vibrio species using whole-cell MALDI-TOF mass spectrometry[J]. Journal of Applied Microbiology,2010,109(1):199-211.
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