Ultrasonic Extraction of <i>β</i>-Carotene from <i>Spirulina platensis</i> in Aqueous Two-phase System and Its Anti-glycosylation Effect

Yin WANG; Xifeng ZHANG; Guanghong LUO

doi:10.13386/j.issn1002-0306.2020060090

Volume 42 Issue 8

Apr. 2021

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Science and Technology of Food Industry > 2021 > 42(8): 152-157. > DOI: 10.13386/j.issn1002-0306.2020060090

WANG Yin, ZHANG Xifeng, LUO Guanghong. Ultrasonic Extraction of β -Carotene from Spirulina platensis in Aqueous Two-phase System and Its Anti-glycosylation Effect[J]. Science and Technology of Food Industry, 2021, 42(8): 152−157. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060090.

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Ultrasonic Extraction of β-Carotene from Spirulina platensis in Aqueous Two-phase System and Its Anti-glycosylation Effect

1.
Faculty of Science, Linfen College of Shanxi Normal University, Linfen 041000, China
2.
Gansu Engineering Technology Research Center for Microalgae, Hexi University, Zhangye 734000, China

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Received Date: June 07, 2020
Available Online: January 27, 2021

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Abstract

Abstract

The optimum extraction conditions of β-carotene from Spirulina platensis by ultrasonic assisted organic solvent / sugar aqueous two-phase system were studied, and its anti glycosylation effect was analyzed. On the basis of determining the extraction system as tert butyl alcohol/maltose aqueous two-phase system, the amount of Spirulina platensis powder, ultrasonic time and ultrasonic power were taken as independent variables, and the yield of β-carotene was taken as dependent variable. The results showed that, in the system of 3.4 g tert-butanol/2.4 g maltose, the amount of Spirulina powder was 0.05 g, fill up with water to 10g, under the ultrasonic power of 90 W and ultrasonic time of 5 min, the extraction yield of β-carotene from Spirulina was 3.19 mg/g. When β-carotene concentration was at ranges of 50 to 450 μg/mL, the highest inhibition rate of AGEs was 47.28%, which could provide a reference for the development of novel AGEs inhibitors.
- β-carotene,
- Spirulina platensis,
- ultrasonic extraction,
- t-butanol/maltose system,
- anti-glycosylation

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References

[1]	Walker T, Purton S, Becker D, et al. Microalgae as bioreactors[J]. Plant Cell Reports,2005,24(11):629−641. doi: 10.1007/s00299-005-0004-6
[2]	Moraes C C, Jf D M B, Kalil S J. C-phycocyanin extraction process for large-scale use[J]. Journal of Food Biochemistry,2010,34(s1):133−148.
[3]	Ma Q, Fang M, Zheng J, et al. Optimized extraction of β-carotene from Spirulina platensis and hypoglycaemic effect in streptozotocin-induced diabetic mice[J]. Journal of the Science of Food and Agriculture,2016,96(5):1783−1789. doi: 10.1002/jsfa.7286
[4]	Dey S, Rathod V K. Ultrasound assisted extraction of β-carotene from Spirulina platensis[J]. Ultrasonics Sonochemistry,2013,20(1):271−276. doi: 10.1016/j.ultsonch.2012.05.010
[5]	Yahia E M, de Jesús Ornelas-Paz J, Emanuelli T, et al. Chemistry, stability, and biological actions of carotenoids[J]. Fruit and Vegetable Phytochemicals: Chemistry and Human Health,2017,2:285.
[6]	Szpylka J, De Vries J W. Determination of β-carotene in supplements and raw materials by reversed-phase high pressure liquid chromatography: Collaborative study[J]. Journal of AOAC International,2005,88(5):1279−1291. doi: 10.1093/jaoac/88.5.1279
[7]	Ford E S, Will J C, Bowman B A, et al. Diabetes mellitus and serum carotenoids: Findings from the third national health and nutrition examination survey[J]. American Journal of Epidemiology,1999,149(2):168−176. doi: 10.1093/oxfordjournals.aje.a009783
[8]	赵楠, 李勇勇, 邵明飞, 等. 螺旋藻粉β-胡萝卜素含量测定方法的优化[J]. 菏泽学院学报,2012(5):36−40.
[9]	廖传华, 朱廷风. 螺旋藻中β-胡萝卜素的超临界CO₂萃取实验研究[J]. 粮油加工与食品机械,2003(4):66−67.
[10]	张喜峰, 杨生辉, 罗光宏, 等. (乙醇+丙酮)/硫酸铵二元双水相萃取分离螺旋藻中β-胡萝卜素[J]. 天然产物研究与开发,2015(3):393−397.
[11]	Wang B, Ezejias T, Feng H, et al. Sugaring-out: A novel phase separation and extraction system[J]. Chemical Engineering Science,2008,63(9):2595−2600. doi: 10.1016/j.ces.2008.02.004
[12]	孜力汗, 康凯, 牟英, 等. 糖析萃取与发酵耦合分离发酵液中乙醇初探[J]. 中国科学: 化学,2015(5):547−557.
[13]	Tsai W H, Chuang H Y, Chen H H, et al. Application of sugaring-out extraction for the determination of sulfonamides in honey by high-performance liquid chromatography with fluorescence detection[J]. Journal of Chromatography A,2010,1217(49):7812−7815. doi: 10.1016/j.chroma.2010.10.008
[14]	de Brito Cardoso G, Mourão T, Pereira F M, et al. Aqueous two-phase systems based on acetonitrile and carbohydrates and their application to the extraction of vanillin[J]. Separation and Purification Technology,2013,104:106−113. doi: 10.1016/j.seppur.2012.11.001
[15]	Wang B, Feng H, Ezeji T, et al. Sugaring-out separation of acetonitrile from its aqueous solution[J]. Chemical Engineering & Technology: Industrial Chemistry-Plant Equipment-Process Engineering-Biotechnology,2008,31(12):1869−1874.
[16]	Zhang C, Huang K, Yu P, et al. Sugaring-out three-liquid-phase extraction and one-step separation of Pt (IV), Pd (II) and Rh (III)[J]. Separation and purification technology,2012,87:127−134. doi: 10.1016/j.seppur.2011.11.032
[17]	刘贵梅, 章鼎敏, 李普, 等. 大豆 7S 蛋白-糖体系晚期糖基化终产物形成因素[J]. 食品科学,2017,38(15):20−25. doi: 10.7506/spkx1002-6630-201715004
[18]	马宗艳, 蔡宏斌, 葛朝明. 晚期糖基化终末产物与阿尔茨海默病的关系[J]. 解放军医学杂志,2018,43(1):79−84.
[19]	吴茜, 董丽红, 李书艺, 等. 莲原花青素对含有不同饱和度脂肪酸的乳糖-赖氨酸体系中晚期糖基化终末产物的抑制作用[J]. 食品科学,2015(13):44−48.
[20]	鞠兴荣, 严梅荣, 三相分离法提取米糠油的初步研究[J]. 中国粮油学报, 2007, 22(6): 125-128.
[21]	张兰威, 陈一, 韩雪, 等. 双水相萃取法从风干香肠中分离提取蛋白酶[J]. 分析化学,2008,36(7):900−904. doi: 10.3321/j.issn:0253-3820.2008.07.007
[22]	Tu Z, Huang T, Wang H, et al. Physico-chemical properties of gelatin from bighead carp (Hypophthalmichthys nobilis) scales by ultrasound-assisted extraction[J]. Journal of Food Science and Technology,2015,52(4):2166−2174. doi: 10.1007/s13197-013-1239-9
[23]	户宪珍, 杨长花, 杨文娟, 等. 响应面法优化超声辅助提取朱砂根中岩白菜素的提取工艺[J]. 西北药学杂志,2019,34(4):458−462. doi: 10.3969/j.issn.1004-2407.2019.04.008
[24]	王露, 李瑾, 权利娜, 等. 西藏绿萝花总多糖提取的动力学模型研究[J]. 中南药学,2019,17(1):30−33.
[25]	赵凯, 牛会平, 侯建平, 等. 香菇多糖提取动力学模型研究[J]. 河北师范大学学报: 自然科学版,2019(1):52−59.
[26]	张喜峰, 张芬琴, 杨生辉, 等. 超声集成乙醇/正丁醇双水相萃取螺旋藻中的 β-胡萝卜素[J]. 食品工业科技,2015,36(17):187−191.
[27]	房明. 螺旋藻 β-胡萝卜素的提取及降血糖活性研究[D]. 北京: 北京林业大学, 2012.
[28]	王海英, 杜为民, 李雯, 等. 螺旋藻干粉中β-胡萝卜素提取工艺研究[J]. 中国食物与营养,2010(6):64−66. doi: 10.3969/j.issn.1006-9577.2010.06.017

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