Effects of One Berry Herbal Beverage on Retinal Cells and Antioxidant Capacity in Optic Nerve Crushed Mice

XU Wenliu; WENG Shaoquan; XU Haining

doi:10.13386/j.issn1002-0306.2020050301

Volume 42 Issue 4

Feb. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(4): 288-294. > DOI: 10.13386/j.issn1002-0306.2020050301

XU Wenliu, WENG Shaoquan, XU Haining. Effects of One Berry Herbal Beverage on Retinal Cells and Antioxidant Capacity in Optic Nerve Crushed Mice[J]. Science and Technology of Food Industry, 2021, 42(4): 288-294. DOI: 10.13386/j.issn1002-0306.2020050301

Citation:

PDF (1603 KB)

Effects of One Berry Herbal Beverage on Retinal Cells and Antioxidant Capacity in Optic Nerve Crushed Mice

Guangzhou Wanglaoji Health Industry Co., Ltd., Guangzhou 510623, China

More Information

Received Date: May 25, 2020
Available Online: March 01, 2021

Graphical Abstract

Abstract

Abstract

To explore the eye protection effect of one berry herbal beverage,optic nerve crush model mice were used. The beverage was administered intragastrically daily for 7 days before and after optic nerve crushed,respectively. The gavage dose of the experimental group was 0.1,1,10,100 mg/kg,and the control group was gavage with phosphate buffer solution. Immunofluorescence staining of retinal stretching preparation,western blot analysis and reactive oxygen species(ROS)fluorescent probe were applied to determine the survival of retinal ganglion cells,retinal protein expression and retinal oxidative stress level of mice after 14 days’ intragastric administration. The results showed that this eye-protecting beverage affected retinal ganglion cells(RGCs)in a dose-dependent manner. Among the 4 dose groups,only 10 mg/kg group significantly increased the survival of retinal ganglion cells(RGCs)by about 8.25% compared with the control group(P<0.05). Under the above-mentioned dosage,there was no significant change observed in the activation and polarization of microglia,a retinal immunoinflammatory cell. But the content of some oxidative stress related proteins in retina,including manganese superoxide dismutase(MnSOD),heme oxygenase 1(HO-1)and nuclear factor erythroid 2-related factor 2(Nrf-2),increased significantly. Meanwhile,the ROS level of retina of experimental group was decreased by about 25.62% and 16.95% for crush injured eye and self-control contralateral eye respectively. Therefore,this beverage could protect eyes by maintaining the activity of retinal ganglion cells,enhancing the antioxidant capacity of retina and reducing the level of reactive oxygen species of retina.
- eye protection efficacy,
- retinal ganglion cells,
- microglia,
- reactive oxygen species,
- anti-oxidative stress,
- medicine food homology,
- wolfberry

FullText(HTML)

References (32)

References

[1]	Li H-Y,Huang M,Luo Q-Y,et al. Lycium barbarum(wolfberry)increases retinal ganglion cell survival and affects both microglia/macrophage polarization and autophagy after rat partial optic nerve transection[J]. Cell Transplantation,2019,28(5):607-618.
[2]	Li H-Y,Ruan Y-W,Kau P W-F,et al. Effect of Lycium barbarum(wolfberry)on alleviating axonal degeneration after partial optic nerve transection[J]. Cell Transplantation,2015,24(3):403-417.
[3]	Liu F,Zhang J,Xiang Z,et al. Lycium barbarum polysaccharides protect retina in rd1 mice during photoreceptor degeneration[J]. Investigative Opthalmology & Visual Science,2018,59(1):597.
[4]	Yang D,So K-F,Lo A C. Lycium barbarum polysaccharide extracts preserve retinal function and attenuate inner retinal neuronal damage in a mouse model of transient retinal ischaemia:Lycium barbarum protect mouse retina[J]. Clinical & Experimental Ophthalmology,2017,45(7):717-729.
[5]	Chen W,Li Y,Bao T,et al. Mulberry fruit extract affords protection against ethyl carbamate-induced cytotoxicity and oxidative stress[J]. Oxidative Medicine and Cellular Longevity,2017,2017:1-12.
[6]	Lee S H,Jeong E,Paik S-S,et al. Cyanidin-3-glucoside extracted from mulberry fruit can reduce N-methyl-N-nitrosourea-induced retinal degeneration in rats[J]. Current Eye Research,2014,39(1):79-87.
[7]	Jang D S,Lee G Y,Kim Y S,et al. Anthraquinones from the seeds of Cassia tora with inhibitory activity on protein glycation and aldose reductase[J]. Biological & Pharmaceutical Bulletin,2007,30(11):2207-2210.
[8]	Skrovankova S,Sumczynski D,Mlcek J,et al. Bioactive compounds and antioxidant activity in different types of berries[J]. International Journal of Molecular Sciences,2015,16(10):24673-24706.
[9]	Zafra-Stone S,Yasmin T,Bagchi M,et al. Berry anthocyanins as novel antioxidants in human health and disease prevention[J]. Molecular Nutrition & Food Research,2007,51(6):675-683.
[10]	Tu L,Sun H,Tang M,et al. Red raspberry extract(Rubus idaeus L shrub)intake ameliorates hyperlipidemia in HFD-induced mice through PPAR signaling pathway[J]. Food and Chemical Toxicology,2019,133:110796.
[11]	Chen L,Li K,Liu Q,et al. Protective effects of raspberry on the oxidative damage in HepG2 cells through Keap1/Nrf2-dependent signaling pathway[J]. Food and Chemical Toxicology,2019,133:110781.
[12]	Li Y,Yang P,Luo Y,et al. Chemical compositions of chrysanthemum teas and their anti-inflammatory and antioxidant properties[J]. Food Chemistry,2019,286:8-16.
[13]	Shen Z,Shao J,Dai J,et al. Diosmetin protects against retinal injury via reduction of DNA damage and oxidative stress[J]. Toxicology Reports,2016,3:78-86.
[14]	Hou B,He S,Gong Y,et al. Effect of obtusifolin administration on retinal capillary cell death and the development of retinopathy in diabetic rats[J]. Cell Biochemistry and Biophysics,2014,70(3):1655-1661.
[15]	Isenmann S. Molecular determinants of retinal ganglion cell development,survival,and regeneration[J]. Progress in Retinal and Eye Research,2003,22(4):483-543.
[16]	Karlstetter M,Scholz R,Rutar M,et al. Retinal microglia:Just bystander or target for therapy?[J]. Progress in Retinal and Eye Research,2015,45:30-57.
[17]	Ahmed Z,Shaw G,Sharma V P,et al. Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry[J]. Journal of Histochemistry & Cytochemistry,2007,55(7):687-700.
[18]	Zhou T,Huang Z,Sun X,et al. Microglia polarization with M1/M2 phenotype changes in rd1 mouse model of retinal degeneration[J]. Frontiers in Neuroanatomy,2017,11:77.
[19]	Cherry J D,Olschowka J A,O'Banion M. Neuroinflammation and M2 microglia:The good,the bad,and the inflamed[J]. Journal of Neuroinflammation,2014,11(1):98.
[20]	Rashid K,Akhtar-Schaefer I,Langmann T. Microglia in retinal degeneration[J]. Frontiers in Immunology,2019,10:1975.
[21]	Nicholls S J. The complex intersection of inflammation and oxidation[J]. Journal of the American College of Cardiology,2008,52(17):1379-1380.
[22]	Strauss O. The retinal pigment epithelium in visual function[J]. Physiological Reviews,2005,85(3):845-881.
[23]	Garg T K,Chang J Y. Oxidative stress causes ERK phosphorylation and cell death in cultured retinal pigment epithelium:Prevention of cell death by AG126 and 15-deoxy-delta 12,14-PGJ2[J]. BMC Ophthalmology,2003,3(1):5.
[24]	Bajpai V K,Alam M B,Quan K T,et al. Antioxidant efficacy and the upregulation of Nrf2-mediated HO-1 expression by(+)-lariciresinol,a lignan isolated from Rubia philippinensis,through the activation of p38[J]. Scientific Reports,2017,7(1):46035.
[25]	Cardoso S. Special Issue:The antioxidant capacities of natural products[J]. Molecules,2019,24(3):492.
[26]	Ung L,Pattamatta U,Carnt N,et al. Oxidative stress and reactive oxygen species:A review of their role in ocular disease[J]. Clinical Science,2017,131(24):2865-2883.
[27]	Amagase H,Sun B,Borek C. Lycium barbarum(goji)juice improves in vivo antioxidant biomarkers in serum of healthy adults[J]. Nutrition Research,2009,29(1):19-25.
[28]	Wang F,Miao M,Xia H,et al. Antioxidant activities of aqueous extracts from 12 Chinese edible flowers in vitro and in vivo[J]. Food & Nutrition Research,2017,61(1):1265324.
[29]	Raman S,Ganeshan A K G,Chen C,et al. In vitro and in vivo antioxidant activity of flavonoid extracted from mulberry fruit(Morus alba L.)[J]. Pharmacognosy Magazine,2016,12(46):128.
[30]	Liu C,Liu Q,Sun J,et al. Extraction of water-soluble polysaccharide and the antioxidant activity from Semen cassiae[J].Journal of Food and Drug Analysis,2014,22(4):492-499.
[31]	Teng H,Fang T,Lin Q,et al. Red raspberry and its anthocyanins:Bioactivity beyond antioxidant capacity[J]. Trends in Food Science & Technology,2017,66:153-165.
[32]	Kalt W,Blumberg J B,McDonald J E,et al. Identification of anthocyanins in the liver,eye,and brain of blueberry-fed pigs[J].Journal of Agricultural and Food Chemistry. 2008,56:705-712.

Supplements (0)

Cited By

Cited by

Periodical cited type(6)

1.	周勇刚. 不同种质资源花椒的品质差异及影响因素分析. 绿色科技. 2024(05): 152-155 .
2.	刘诚诚，蔺雨阳，何玲，杨涛，俞静，沈杰，蒋祺，彭建勇，兰海. 石漠土结构化培肥下青花椒产量性状与光合行为的平衡. 农学学报. 2024(06): 60-66 .
3.	徐丽，朱田田，晋玲，黄钰芳，马晓辉，康舒淇. 基于感官评价和化学计量学的兰州百合品质分析. 食品工业科技. 2024(18): 219-227 . 本站查看
4.	蔡思进，李雅芝，刘慧娟，王耀，谢姣. 低温贮藏对鲜青花椒品质的影响. 中国果菜. 2023(05): 29-34+63 .
5.	曾维友，周於强，池浩. 颗粒度对干制青花椒粉品质的影响. 食品与发酵工业. 2023(20): 265-275 .
6.	严春俊，郭川川，李般程，谢思，马钤，王浩文. 不同品种青花椒品质对比分析. 食品工业. 2022(11): 314-320 .