Optimization of Extraction Technology of Ampelopsin from <i>Ampelopsis grossedentata</i> by Aqueous Two-Phase System

ZHANG Lihua; ZHANG Yuefen; HUANG Renjie

doi:10.13386/j.issn1002-0306.2020050287

Volume 42 Issue 6

Mar. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(6): 181-186,194. > DOI: 10.13386/j.issn1002-0306.2020050287

ZHANG Lihua, ZHANG Yuefen, HUANG Renjie. Optimization of Extraction Technology of Ampelopsin from Ampelopsis grossedentata by Aqueous Two-Phase System[J]. Science and Technology of Food Industry, 2021, 42(6): 181-186,194. DOI: 10.13386/j.issn1002-0306.2020050287

Citation:

PDF (1497 KB)

Optimization of Extraction Technology of Ampelopsin from Ampelopsis grossedentata by Aqueous Two-Phase System

Department of Pharmacy, Fujian Health College, Fuzhou 350101, China

More Information

Received Date: May 24, 2020
Available Online: March 15, 2021

Graphical Abstract

Abstract

Abstract

In this work, the effect of the composition of polyethylene glycol(PEG) -ammonium sulfate aqueous two-phase system on the extraction rate of ampelopsin from Ampelopsis grossedentata was studied. The effects of the molecular weight of PEG, the mass fraction of PEG, the mass fraction of ammonium sulfate, pH and the mass fraction of NaCl on the extraction rate were studied by single factor experiment. Secondly, three factors with significant influence on extraction rate were selected through Plackett-Burman design, namely, mass fraction of PEG, mass fraction of ammonium sulfate and pH. Response surface methodology was used to further optimize the influential factors. The optimum extraction conditions were: PEG1000 mass fraction 28%, ammonium sulfate mass fraction 11.2%, pH6.0. Under this condition, the theoretical extraction rate was 98.82%, and the actual extraction rate was 98.53% after process verification, which was close to the predicted value and 17.53% higher than the extraction rate before optimization(71%), which was conducive to the further separation, purification and application of ampelopsin.
- Ampelopsis grossedentata,
- ampelopsin,
- aqueous two-phase,
- extraction,
- Plackett-Burman design,
- response surface methodology

FullText(HTML)

References (31)

References

[1]	中国科学院研究所.中国高等植物图鉴补编:第2册[M]. 北京:科学出版社,1979:353.
[2]	中国科学院中国植物志编辑委员会. 中国植物志第第十九卷[M]. 北京:科学出版社,1999.
[3]	Qiu P,Dong Y,Li B,et al. Dihydromyricetin modulates p62 and autophagy crosstalk with the Keap-1/Nrf2 pathway to alleviate ethanol-induced hepatic injury[J]. Toxicol Lett,2017,274:31-41.
[4]	周海云,王文清,施春阳,等. 二氢杨梅素药理及药物相互作用研究进展[J]. 中草药,2018,49(14):,3411-3418.
[5]	罗文敏,孙超,向准,等. 藤茶止咳化痰药效学研究[J]. 贵州科学,2014,32(6):58-60.
[6]	Matsumoto T,Tahara S. Ampelopsin,a major antifungal constituent from Salix sachalinensis and its methyl ethers[J]. Nippon Nogeik Kaishi,2001,75(6):659-668.
[7]	邓新武,黄金光,张永强,等. 藤茶防腐抑菌作用的研究[J].生物学通报,2019,54(4):49-52.
[8]	Dalcin A J F,Santos C G,Gundel S S,et al. Anti biofilm effect of dihydromyricetin-loaded nanocapsules on urinary catheter infected by Pseudomonas aeruginosa[J]. Colloids Surf B Biointerfaces,2017,156(5):282-291.
[9]	Jeon,Seong H,Chun,et al. Cyto-toxic constituents from the bark of Salix hulteni[J]. Archives of PharmacalResearch,2008,31(8):978-982.
[10]	Fan K J,Yang B,Liu Y,et al. Inhibition of human lung cancer proliferation through targeting stromal fi-broblasts by dihydromyricetin[J]. Mol Med Rep,2017,16(6):9758-9762.
[11]	Luo G Q,Zeng S,Liu D Y. Inhibitory Effects of Ampelopsin on Angiogenesis[J]. Journal of Chinese Medicinal Materials,2006,29(2):146-150.
[12]	谭胜蓝,陈磊,何桂霞,等. 二氢杨梅素心血管保护作用的研究进展[J]. 中南药学,2017,15(3):339-343.
[13]	李自强,覃丽,牛丽,等. 藤茶对心血管保护作用的研究进展[J]. 食品科技,2019,44(5):97-101.
[14]	逯凤肖,郁建平,秦湉,等. 藤茶中二氢杨梅素对糖尿病小鼠血糖和血脂的影响[J]. 山地农业生物学报,2015,34(6):89-92.
[15]	熊伟,王慧宾,李雄辉,等. 热水浸提法同步提取藤茶中二氢杨梅素和多糖的工艺研究[J]. 生物化工,2015,1(1):5-6 ,11.
[16]	肖浩,郑小江. 响应面法优化发酵藤茶黄酮和多糖的提取工艺[J]. 湖北民族学院学报(自然科学版),2013,31(2):132-137.
[17]	陈玉琼,李安琪,孟燕. 藤茶黄酮及二氢杨梅素提取条件的优化[J]. 华中农业大学学报,2009,28(1):106-110.
[18]	宾冬梅,易诚,颜彩虹. 微波辅助法提取藤茶黄酮工艺优化[J]. 安徽农业科学,2014,42(27):9320-9321 ,9403.
[19]	雷燕妮,张小斌,李多伟. 显齿蛇葡萄叶中二氢杨梅素超声提取工艺的优化[J]. 贵州农业科学,2018,46(5):123-126.
[20]	陈雁梅,于华忠,刘同方,等. 酶法提取藤茶茎中二氢杨梅素工艺研究[J]. 应用化工,2016,45(2):304-307.
[21]	严赞开. 二氢杨梅素的分离研究进展[J]. 安徽农业科学,2008,36(34):14834-14836.
[22]	Rahimpour F,Hatti-Kaul R,Mamo G. Response surface methodology and artificial neural network modelling of an aqueous two-phase system for purification of a recombinant alkaline active xylanase[J]. Process Biochemistry,2016,51(3):452-462.
[23]	钟方丽,王文姣,王晓林,等. 刺玫果总黄酮的双水相萃取工艺及其抗氧化能力[J]. 林产化学与工业,2016,36(4):64-72.
[24]	Naveena B J,Altaf M,Bhadria K,et al. Bioresource Technology[J]. 2005,96(4):485.
[25]	Kasiri M B,Aleboyeh H,Aleboyeh A. Modeling and optimization of heterogeneous photo-fenton process with response surface methodology and artifcial neural networks[J]. Environmental Science and Technology,2008,42(21):7970-7975.
[26]	裴莹,孙玉波,黄斌. 双水相萃取分离α-酮戊二酸[J]. 食品与发酵工业,2018,44(12):150-154.
[27]	李化,柯华香,李发洁,等. Box-Behnken响应面法优选五味子多糖双水相提取工艺[J]. 中药材,2016,39(3):593-597.
[28]	袁雷,钟政昌,刘瑜,等. 响应面法优化血满草多糖双水相萃取工艺[J]. 安徽农业科学,2020,48(13):179-182 ,234.
[29]	罗光宏,张喜峰,张丽娟,等. 响应面法优化双水相萃取棉花叶中总黄酮的研究[J]. 食品工业科技,2014,35(16):196-200.
[30]	汪建红,廖立敏,王碧. 乙醇-硫酸铵双水相体系提取柠檬渣中总黄酮研究[J]. 华中师范大学学报,2013,47(1):78-81.
[31]	钟玲,张越非,李小菊,等. 乙醇/无机盐双水相体系分离纯化黄芪总黄酮的研究[J]. 中国中药杂志,2012,37(22):3395-3399.