Study on <i>In Vivo</i> and <i>In Vitro</i> Antioxidant Activity of Collagen Oligopeptide Chelated Zinc from Puffer Skin

GUO Honghui; ZHANG Yiping; HONG Zhuan; YU Yun

doi:10.13386/j.issn1002-0306.2020050219

Volume 42 Issue 5

Feb. 2021

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Science and Technology of Food Industry > 2021 > 42(5): 66-71. > DOI: 10.13386/j.issn1002-0306.2020050219

GUO Honghui, ZHANG Yiping, HONG Zhuan, YU Yun. Study on In Vivo and In Vitro Antioxidant Activity of Collagen Oligopeptide Chelated Zinc from Puffer Skin[J]. Science and Technology of Food Industry, 2021, 42(5): 66-71. DOI: 10.13386/j.issn1002-0306.2020050219

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Study on In Vivo and In Vitro Antioxidant Activity of Collagen Oligopeptide Chelated Zinc from Puffer Skin

1. Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China;
2. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

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Received Date: May 19, 2020
Available Online: March 02, 2021

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Abstract

Abstract

Collagen oligopeptide chelated zinc was prepared from puffer skin. Its composition and structure were characterized. Taking 1,1-diphenyl-2-trinitrobenzene hydrazine free radical(DPPH·),hydroxyl free radical(·OH)scavenging ability,ferric iron(Fe³⁺)reducing power as indicators,the in vitro antioxidant capacity of collagen oligopeptide chelated zinc was evaluated. The experimental results showed that both collagen peptide and zinc chelate had DPPH free radical and hydroxyl free radicals scavenging activities,and ferric iron(Fe³⁺)reducing power. They also had good dose-effect relationship with the sample concentration. Collagen oligopeptide chelated zinc showed greater antioxidant ability than collagen oligopeptide. Collagen oligopeptide chelated zinc could significantly improve the total antioxidant capacity(T-AOC),glutathione(GSH)and total superoxide dismutase(T-SOD)activity in the serum of Zn-deficient rats,and obviously reduce the content of malondialdehyde(MDA)(P<0.05,0.01). Collagen oligopeptide chelated zinc from puffer skin has excellent antioxidant activity in vitro and in vivo,which is expected to be used as a new antioxidant in biological products and food industry.
- puffer skin,
- collagen oligopeptide chelated zinc,
- antioxidant activity,
- animal experiment,
- in vivo,
- in vitro

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Science and Technology of Food Industry

Study on In Vivo and In Vitro Antioxidant Activity of Collagen Oligopeptide Chelated Zinc from Puffer Skin