Study on the Test of Sodium Alginate Lyase Activity

Mingliang ZHENG; Hunlong ZHENG; Chun MENG; Hang WANG

doi:10.13386/j.issn1002-0306.2020050180

Volume 42 Issue 7

Mar. 2021

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Science and Technology of Food Industry > 2021 > 42(7): 246-251. > DOI: 10.13386/j.issn1002-0306.2020050180

ZHENG Mingliang, ZHENG Hunlong, MENG Chun, et al. Study on the Test of Sodium Alginate Lyase Activity [J]. Science and Technology of Food Industry, 2021, 42(7): 246−251. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020050180.

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Study on the Test of Sodium Alginate Lyase Activity

1.
Research Institute of Photocatalysis, College of Biological Science and Engineering, Fuzhou University, Fuzhou 350116, China
2.
Fujian Bokai Technology Co., LTD, Fuzhou 350108, China

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Received Date: May 17, 2020
Available Online: January 27, 2021

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Abstract

Abstract

Based on the principle of enzyme reaction kinetics, the reaction system and conditions for the test of enzyme activity of sodium alginate lyase were studied in order to improve the test method of sodium alginate lyase. In this experiment, the enzyme activity was measured by ultraviolet absorption method, and the improved method was as follows: 300 μL of substrate solution (sodium alginate 2.2%, KCl 5 mmol/L, 0.1 mol/L Na₂HPO₄-NaH₂PO₄ buffer, pH7.0) and 50 μL of enzyme solution diluted to 2.4～30 U/mL was mixed and reacted in a 37 ℃ water bath for 20 minutes, and then the reaction was terminated with an ice bath. After the reaction solution was diluted 20 times, the absorbance was measured at 235 nm. Each substrate solution need to be removed with a new pipette tip to improve the precision, and the pipette error was less than 2%. The relative standard deviation of this enzyme activity determination method was less than 5%.
- sodium alginate lyase,
- enzyme reaction kinetics,
- improvement,
- enzyme activity measurement

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References (31)

References

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