Optimization of Extraction by BP-ANN and Determination of Flavonoids in Three Varieties of Polygonati Rhizoma Before and After Irradiation by HPLC Chromatographic Fingerprint

XU Sheng; QU Zengyi; BI Jianli; LI Tingting; GAN Xiaofeng; WEI Guoliang; DU Jifu; HE Min; FAN Baolei

doi:10.13386/j.issn1002-0306.2020050171

Volume 42 Issue 5

Feb. 2021

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Science and Technology of Food Industry > 2021 > 42(5): 257-264. > DOI: 10.13386/j.issn1002-0306.2020050171

XU Sheng, QU Zengyi, BI Jianli, LI Tingting, GAN Xiaofeng, WEI Guoliang, DU Jifu, HE Min, FAN Baolei. Optimization of Extraction by BP-ANN and Determination of Flavonoids in Three Varieties of Polygonati Rhizoma Before and After Irradiation by HPLC Chromatographic Fingerprint[J]. Science and Technology of Food Industry, 2021, 42(5): 257-264. DOI: 10.13386/j.issn1002-0306.2020050171

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Optimization of Extraction by BP-ANN and Determination of Flavonoids in Three Varieties of Polygonati Rhizoma Before and After Irradiation by HPLC Chromatographic Fingerprint

1. College of Pharmacy, Hubei University of Science and Technology, Xianning 437000, China;
2. Market Supervision and Administration Bureau of Xian'an District, Xianning 437000, China;
3. College of Nuclear Technology and Chemical Biology, Hubei University of Science and Technology, Xianning 437000, China;
4. Yufeng Ecological Agriculture Development Co., Ltd., Tongcheng 437400, China

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Received Date: May 17, 2020
Available Online: March 02, 2021

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Abstract

Abstract

Objective:Back propagation artificial neural network(BP-ANN)was designed to optimize the yield rate of seven flavonoids from Polygonatum sibiricum,Polygonatum odoratum and Polygonatum filipes,to determine the content changes of flavonoids before and after electron beam irradiation of different varieties of Polygonati Rhizoma,and to establish chromatographic fingerprint. Methods:The determination was performed by high performance liquid chromatography(HPLC),Inertsil ODS-3,C₁₈ column(250 mm×4.6 mm,5 μm)was used,the mobile phase consisted of 0.02% phosphoric acid(A)-acetonitrile(B),gradient elution at a flow rate of 1.2 mL/min,and the column temperature was 43 ℃,the detection wavelength was set at 270 nm. The fingerprint of Polygonati Rhizoma flavonoids under different irradiation doses was established,and the content changes of Polygonati Rhizoma flavonoids under different irradiation doses were compared. Results:The established HPLC method could accurately determine the contents of rutin,myricetin,liquiritigenin,quercetin,apigenin,kaempferol and isorhamnetin in Polygonati Rhizoma. All the 7 chemical constituents in the Polygonati Rhizoma could produce complete peaks,with the separation degree was more than 1.5,and the components had a good linear relationship within the corresponding concentration range(R²>0.999),and the recovery rate was 97.95%~103.71%. The optimized extraction results by BP-ANN technology showed that under the conditions of solid-liquid ratio of 1:50,extraction time of 2 h,extraction temperature of 90 ℃,and ethanol concentration of 70%,the maximum extraction efficiency(0.0622%)of flavonoids from Polygonati Rhizoma could be obtained. The results of the sample determination showed that only the Polygonatum sibiricum contained all 7 kinds of flavonoids,while the flavonoids in the other two kinds of Polygonati Rhizoma were not the same. The content of flavonoids decreased with the increase of irradiation dose. Conclusion:BP-ANN has high precision and good fitting effect to fit yield rate of flavonoids in Polygonati Rhizoma. The HPLC for the determination of seven flavonoids in Polygonati Rhizoma has high sensitivity and specificity,and can be used for the determination of flavonoids in Polygonati Rhizoma.
- HPLC,
- Polygonati Rhizoma,
- flavonoids,
- electron beam irradiation,,
- BP-ANN

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References

[1]	国家药典委员会.中华人民共和国药典[M]. 2015年版. 一部. 北京:中国医药科技出版社,2015:306-307.
[2]	Zhao X Y,Li J.Chemical constituents of the genus Polygonatum and their role in medicinal treatment[J].Natural Product Communications,2015,10(4):683-688.
[3]	Kumar Singh S,Patra A.Evaluation of phenolic composition,antioxidant,anti-inflammatory and anticancer activities of Polygonatum verticillatum(L.)[J].Journal of Integrative Medicine,2018,16(4):273-282.
[4]	甘小凤,韦国良,李婷婷,等.基于RSM及PMP-HPLC特征图谱分析黄精炮制过程中多糖组分含量变化[J].中草药,2019,50(20):4932-4941.
[5]	Ganbaatar C,Gruner M,Mishig D,et al. Flavonoid glycosides from the aerial parts of Polygonatum odoratum(mill.)druce growing in Mongolia[J].The Open Natural Products Journal,2015,8(1):1-7.
[6]	Sharma S,Joshi R,Kumar D.Quantitative analysis of flavonols,flavonol glycoside and homoisoflavonoids in Polygonatum verticillatum using UHPLC-DAD-QTOF-IMS and evaluation of their antioxidant potential[J].Phytochemical Analysis,2020,31(3):333-339.
[7]	Wang H J,Fowler M I,Messenger D J,et al. Homoisoflavonoids are potent glucose transporter 2(GLUT2)inhibitors:A potential mechanism for the glucose-lowering properties of Polygonatum odoratum[J].Journal of Agricultural and Food Chemistry,2018,66(12):3137-3145.
[8]	胡本祥,彭亮,杨冰月,等."秦药"的现代研究概况[J].中草药,2018,49(21):4949-4959.
[9]	高颖,戚楚露,张磊,等.黄精新鲜药材的化学成分[J].药学与临床研究,2015,23(4):365-367.
[10]	陶爱恩,张晓灿,杜泽飞,等.黄精属植物中黄酮类化合物及其药理活性研究进展[J].中草药,2018,49(9):2163-2171.
[11]	Fathi F M,Harita H,Mahmood A A,et al. Acceleration of wound healing activity by Polygonatum odoratum leaf extract in rats[J].Journal of Medicinal Plants Research,2014,8(13):523-528.
[12]	范林林,韩鹏祥,冯叙桥,等.电子束辐照技术在食品工业中应用的研究与进展[J].食品工业科技,2014,35(14):374-380.
[13]	Pillai S D,Shayanfar S.Electron beam technology and other irradiation technology applications in the food industry[J].Topics in Current Chemistry,2017,375:6.
[14]	Teets A S,Minardi C S,Sundararaman M,et al. Extraction,identification,and quantification of flavonoids and phenolic acids in electron beam-irradiated almond skin powder[J].Journal of Food Science,2009,74(3):C298-C305.
[15]	Nam H A,Ramakrishnan S R,Kwon J H.Effects of electron-beam irradiation on the quality characteristics of mandarin oranges(Citrus unshiu(Swingle)Marcov)during storage[J].Food Chemistry,2019,286:338-345.
[16]	Koike A,Barreira J C M,Barros L,et al. Edible flowers of Viola tricolor L.as a new functional food:Antioxidant activity,individual phenolics and effects of gamma and electron-beam irradiation[J].Food Chemistry,2015,179:6-14.
[17]	Villavicencio A L C H,Heleno S A,Calhelha R C,et al. The influence of electron beam radiation in the nutritional value,chemical composition and bioactivities of edible flowers of Bauhinia variegata L.var.Candida alba Buch.-Ham from Brazil[J].Food Chemistry,2018,241:163-170.
[18]	王文君,向灿辉,刘成红.黄精总黄酮的提取与性质分析[J].食品工业,2014,35(10):258-262.
[19]	Hou M Y,Hu W Z,Wang A S,et al. Ultrasound-assisted extraction of total flavonoids from Pteris cretica L.:Process optimization,HPLC analysis,and evaluation of antioxidant activity[J].Antioxidants,2019,8(10):425.
[20]	Niu Q,Gao Y M,Liu P H.Optimization of microwave-assisted extraction,antioxidant capacity,and characterization of total flavonoids from the leaves of Alpinia oxyphylla Miq[J]. Preparative Biochemistry & Biotechnology,2020,50(1):82-90.
[21]	刘金宇,李艳芳,范建伟,等.HPLC同时测定茵栀黄颗粒中4种黄酮类成分[J].药学研究,2019,38(1):19-21 ,29.
[22]	冯淳,张妮,周大颖,等.HPLC测定显齿蛇葡萄叶中4种黄酮类化合物的含量[J].食品工业科技,2018,39(24):240-245.
[23]	宋晓芳,范宝磊,曾祥玲,等.HPLC-MS/MS法同时测定不同桂花品种中7个多酚类活性成分的含量[J].药物分析杂志,2019,39(10):1811-1820.
[24]	Du K Z,Li J,Guo X R,et al. Quantitative analysis of phenolic acids and flavonoids in Cuscuta chinensis Lam.by synchronous ultrasonic-assisted extraction with response surface methodology[J].Journal of Analytical Methods in Chemistry,2018,2018:1-10.
[25]	Hu X,Zhao H D,Shi S Y,et al. Sensitive characterization of polyphenolic antioxidants in Polygonatum odoratum by selective solid phase extraction and high performance liquid chromatography-diode array detector-quadrupole time-of-flight tandem mass spectrometry[J].Journal of Pharmaceutical and Biomedical Analysis,2015,112:15-22.
[26]	Caprioli G,Nzekoue F K,Giusti F,et al. Optimization of an extraction method for the simultaneous quantification of sixteen polyphenols in thirty-one pulse samples by using HPLC-MS/MS dynamic-MRM triple quadrupole[J].Food Chemistry,2018,266:490-497.
[27]	Luo Y,Wen Q,Lai C J S,et al. Characterization of polymeric phenolic acids and flavonoids in Clerodendranthi Spicati Herba using ultrahigh-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry with target and nontarget data mining strategy[J].Rapid Communications in Mass Spectrometry,2019,33(24):1884-1893.
[28]	侯召华,张宇,金春爱,等.基于人工神经网络的黑米花色苷提取的研究[J].食品科技,2012,37(1):194-198 ,203.
[29]	Mei Y,Yang J Q,Lu Y,et al. BP-ANN model coupled with particle swarm optimization for the efficient prediction of 2-chlorophenol removal in an electro-oxidation system[J]. International Journal of Environmental Research and Public Health,2019,16(14):2454.
[30]	Manda A,Walker R B,Khamanga S M,et al. An artificial neural network approach to predict the effects of formulation and process variables on prednisone release from a multipartite system[J]. Pharmaceutics,2019,11(3):109-126.
[31]	Dou M,He X H,Sun Y,et al. Controlled acid hydrolysis and kinetics of flavone C-glycosides from trollflowers[J]. Chinese Chemical Letters,2015,26(2):255-258.
[32]	Zhou W,Yang Z H,Huang S,et al. Rapid quantitative analysis of ginkgo flavonoids using paper spray mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis,2019,171:158-163.
[33]	Ranjbar Banforuzi S,Hadjmohammadi M R.Two-phase hollow fiber-liquid microextraction based on reverse micelle for the determination of quercetin in human plasma and vegetables samples[J].Talanta,2017,173:14-21.

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