ZHANG Zhihua, ZHENG Huanyu, YAN Guosen, SUN Meixin, XU Hui, CHEN Hao. Research Progress of Allergenic Soybean Protein P34 and Its Removal Method[J]. Science and Technology of Food Industry, 2021, 42(5): 363-368. DOI: 10.13386/j.issn1002-0306.2020050030
Citation: ZHANG Zhihua, ZHENG Huanyu, YAN Guosen, SUN Meixin, XU Hui, CHEN Hao. Research Progress of Allergenic Soybean Protein P34 and Its Removal Method[J]. Science and Technology of Food Industry, 2021, 42(5): 363-368. DOI: 10.13386/j.issn1002-0306.2020050030

Research Progress of Allergenic Soybean Protein P34 and Its Removal Method

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  • Received Date: May 05, 2020
  • Available Online: March 02, 2021
  • As one of the "eight major" allergic foods,soybeans account for about 25% of the total number of food allergies. How to reduce the allergenicity of soybean products and ensure consumer safety has become a hot topic in the field of food safety. Soybean contains a variety of allergenic factors,such as Gly-m Bd 30K protein,Gly-m Bd 28K protein and Gly-m Bd 28K protein. Among them,Gly-m Bd 30K protein,as the most sensitive allergen,can be recognized by the serum of 65% of soybean allergic patients and occupies a prominent position among several allergic proteins. In this paper the research situation of the antigenic epitopes and removal methods of Gly-m Bd 30K protein are reviewed. Among them,enzymatic treatment,ultra-high pressure method,extrusion and expansion and other treatments have made certain achievements in reducing the allergenicity of soybeans. This provides a scientific reference for further improving the quality of soybeans and their products and ensuring their safe use in the food industry and animal husbandry.
  • [1]
    Huang T,Bu G H,Chen F S.The influence of composite enzymatic hydrolysis on the antigenicity of β-conglycinin in soy protein hydrolysates[J].Journal of Food Biochemistry,2018,42(5):e12544.
    [2]
    Meinlschmidt P,Sussmann D,Schweiggert-Weisz U,et al. Enzymatic treatment of soy protein isolates:Effects on the potential allergenicity,technofunctionality,and sensory properties[J].Food Science & Nutrition,2016,4(1):11-23.
    [3]
    Xi J,He M X.High hydrostatic pressure(HHP)effects on antigenicity and structural properties of soybean β-conglycinin[J].Journal of Food Science and Technology,2018,55(2):630-637.
    [4]
    Saeed H,Gagnon C,Cober E,et al. Using patient serum to epitope map soybean glycinins reveals common epitopes shared with many legumes and tree nuts[J].Molecular Immunology,2016,70:125-133.
    [5]
    Watanabe D,Adányi N,Takács K,et al. Development of soybeans with low P34 allergen protein concentration for reduced allergenicity of soy foods[J].Journal of the Science of Food and Agriculture,2017,97(3):1010-1017.
    [6]
    李堂昊,布冠好,陈复生.大豆主要过敏原β-伴大豆球蛋白及其抗原表位的研究进展[J].大豆科学,2019,38(5):806-812.
    [7]
    邓涵. 转谷氨酰胺酶交联协同热加工对豆腐品质特性和潜在致敏性的影响[D]. 南昌:南昌大学,2018.
    [8]
    Ballmer-Weber B K,Vieths S.Soy allergy in perspective[J]. Current Opinion in Allergy and Clinical Immunology,2008,8(3):270-275.
    [9]
    Rouquié D,Capt A,Eby W H,et al. Investigation of endogenous soybean food allergens by using a 2-dimensional gel electrophoresis approach[J].Regulatory Toxicology and Pharmacology,2010,58(3 Suppl):S47-S53.
    [10]
    Bando N,Tsuji H,Yamanishi R,et al. Identification of the glycosylation site of a major soybean allergen,Gly m Bd 30K[J]. Bioscience,Biotechnology,and Biochemistry,1996,60(2):347-348.
    [11]
    Liu B,Teng D,Wang X M,et al. Expression of the soybean allergenic protein P34 in Escherichia coli and its indirect ELISA detection method[J].Applied Microbiology and Biotechnology,2012,94(5):1337-1345.
    [12]
    郑环宇,邵红梅,赵丹丹,等.超高压对风味蛋白酶酶解大豆分离蛋白中P34免疫活性的影响[J].食品科学,2015,36(13):95-100.
    [13]
    李玲梅.大豆糖蛋白N-糖基化分析及其致敏性研究[D].西安:西北大学,2017.
    [14]
    Hiemori M,Bando N,Ogawa T,et al. Occurrence of IgE antibody-recognizing N-linked glycan moiety of a soybean allergen,Gly m Bd 28K[J].International Archives of Allergy and Immunology,2000,122(4):238-245.
    [15]
    Morita H,Kaneko H,Ohnishi H,et al. Structural property of soybean protein P34 and specific IgE response to recombinant P34 in patients with soybean allergy[J].International Journal of Molecular Medicine,2012,29(2):153-158.
    [16]
    Koppelman S J,Hefle S L.Detecting allergens in food[M]. Woodhead Publishing Limited,2006.
    [17]
    Pierboni E,Rondini C,Torricelli M,et al. Digital PCR for analysis of peanut and soybean allergens in foods[J].Food Control,2018,92:128-136.
    [18]
    Bu G H,Huang T,Li T H.The separation and identification of the residual antigenic fragments in soy protein hydrolysates[J].Journal of Food Biochemistry,2020,44(3):e13144.
    [19]
    林苏霞,王晓梅,刘志刚,等.大豆主要过敏原Glym Bd 30K的抗原表位区基因的克隆表达、纯化及免疫原性鉴定[J].大豆科学,2010,29(2):186-190.
    [20]
    Candreva A M,Smaldini P L,Curciarello R,et al. Cross-reactivity between the soybean protein P34 and bovine caseins[J].Allergy,Asthma & Immunology Research,2015,7(1):60.
    [21]
    Wang W,Zhu X D,Teng S,et al. Label-free biochips for rapid detection of soybean allergen GlymBd 30K(P34)in foods[J].Tropical Journal of Pharmaceutical Research,2017,16(4):755.
    [22]
    Takahashi M,Uematsu Y,Kashiwaba K,et al. Accumulation of high levels of free amino acids in soybean seeds through integration of mutations conferring seed protein deficiency[J]. Planta,2003,217(4):577-586.
    [23]
    Herman E.Soybean allergenicity and suppression of the immunodominant allergen[J].Crop Science,2005,45(2):462-467.
    [24]
    尹红.美国科学家首次成功培育过敏性低大豆品种[J]. 粮食与油脂,2003,16(1):22.
    [25]
    徐圣捷,赵东,高祥,等.超高压食品加工设备现状及发展趋势[J].食品工业,2019,40(12):222-225.
    [26]
    Zhang Y F,Ren Y Y,Bi Y G,et al. Review:Seafood allergy and potential application of high hydrostatic pressure to reduce seafood allergenicity[J].International Journal of Food Engineering,2019,15(8):1-12.
    [27]
    Li H J,Zhu K X,Zhou H M,et al. Effects of high hydrostatic pressure treatment on allergenicity and structural properties of soybean protein isolate for infant formula[J].Food Chemistry,2012,132(2):808-814.
    [28]
    郑环宇,董小超,白小娟,等.超高压处理对大豆蛋白致敏原P34免疫活性的影响研究[J].食品工业科技,2014,35(24):152-155.
    [29]
    Li H J,Jia Y M,Peng W,et al. High hydrostatic pressure reducing allergenicity of soy protein isolate for infant formula evaluated by ELISA and proteomics via Chinese soy-allergic children's sera[J].Food Chemistry,2018,269:311-317.
    [30]
    金征宇,赵建伟.不同温度湿法挤压对全脂大豆成分及抗营养因子的影响[J].饲料与畜牧,2008(9):7-9.
    [31]
    Franck P,Moneret Vautrin D A,Dousset B,et al. The allergenicity of soybean-based products is modified by food technologies[J].International Archives of Allergy and Immunology,2002,128(3):212-219.
    [32]
    郑环宇.挤压膨化工艺对大豆分离蛋白-玉米和脱脂大豆粉玉米膨化产品中致敏因子免疫活性的影响[A].中国食品科学技术学会第九届年会论文摘要集[C].中国食品科学技术学会:中国食品科学技术学会,2012:2.
    [33]
    Rahaman T,Vasiljevic T,Ramchandran L.Effect of processing on conformational changes of food proteins related to allergenicity[J].Trends in Food Science & Technology,2016,49:24-34.
    [34]
    刘晓毅.大豆食源性致敏蛋白的识别、去除及脱敏后加工特性研究[D].北京:中国农业大学,2005.
    [35]
    Wilson S,Martinez-Villaluenga C,De Mejia E G.Purification,thermal stability,and antigenicity of the immunodominant soybean allergen P34 in soy cultivars,ingredients,and products[J].Journal of Food Science,2008,73(6):T106-T114.
    [36]
    Samoto M,Akasaka T,Mori H,et al. Simple and efficient procedure for removing the 34kDa allergenic soybean protein,gly m I,from defatted soy milk[J].Bioscience,Biotechnology,and Biochemistry,1994,58(11):2123-2125.
    [37]
    Zhang Q,Li L,Lan Q Y,et al. Protein glycosylation:A promising way to modify the functional properties and extend the application in food system[J].Critical Reviews in Food Science and Nutrition,2019,59(15):2506-2533.
    [38]
    Babiker E F E,Hiroyuki A,Matsudomi N,et al. Effect of polysaccharide conjugation or transglutaminase treatment on the allergenicity and functional properties of soy protein[J]. Journal of Agricultural & Food Chemistry,1998,46(3):866-871.
    [39]
    Wilson S,Blaschek K,De Mejia E G.Allergenic proteins in soybean:processing and reduction of P34 allergenicity[J]. Nutrition Reviews,2005,63(2):47-58.
    [40]
    朱婷伟.糖基化修饰对大豆蛋白过敏原抗原性的影响研究[D]. 郑州:河南工业大学,2015.
    [41]
    Frias J,Song Y S,Martínez-Villaluenga C,et al. Immunoreactivity and amino acid content of fermented soybean products[J].Journal of Agricultural and Food Chemistry,2008,56(1):99-105.
    [42]
    Yang A S,Zuo L L,Cheng Y F,et al. Degradation of major allergens and allergenicity reduction of soybean meal through solid-state fermentation with microorganisms[J].Food & Function,2018,9(3):1899-1909.
    [43]
    Yamanishi R,Tsuji H,Bando N,et al. Reduction of the allergenicity of soybean by treatment with proteases[J].Journal of Nutritional Science and Vitaminology,1996,42(6):581-587.
    [44]
    郑环宇,白小娟,张丽丽,等.酶水解对大豆致敏蛋白P34免疫活性的影响及酶解产物理化性质研究[J].东北农业大学学报,2014,45(6):6-15.
    [45]
    Tian S L,Ma J J,Ahmed I,et al. Effect of tyrosinase-catalyzed crosslinking on the structure and allergenicity of turbot parvalbumin mediated by caffeic acid[J].Journal of the Science of Food and Agriculture,2019,99(7):3501-3508.
    [46]
    Kasera R,Singh A B,Kumar R,et al. Effect of thermal processing and γ-irradiation on allergenicity of legume proteins[J].Food and Chemical Toxicology:An International Journal Published for the British Industrial Biological Research Association,2012,50(10):3456-3461.
    [47]
    Meinlschmidt P,Brode V,Sevenich R,et al. High pressure processing assisted enzymatic hydrolysis-An innovative approach for the reduction of soy immunoreactivity[J].Innovative Food Science & Emerging Technologies,2017,40:58-67.
    [48]
    范志军,陈龙,于爱华,等.微射流辅助酶解制备低敏性豆粉的工艺优化[J].食品科技,2019,44(5):59-65.
    [49]
    祖琴琴. 超声协同酶解去糖基化处理对大豆7S蛋白结构及致敏性的影响[D].南昌:南昌大学,2015.
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