Evaluation and Analysis of the Colloidal Gold Immunochromatography Kits for the Rapid Detection of Pesticide Residue

YE Qiuxiong; LIANG Junfa; ZHANG Binbin; LIN Jiajian; PENG Cheng; ZENG Chengzhu; HE Liyuan

doi:10.13386/j.issn1002-0306.2020040355

Volume 42 Issue 4

Feb. 2021

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Science and Technology of Food Industry > 2021 > 42(4): 187-193. > DOI: 10.13386/j.issn1002-0306.2020040355

YE Qiuxiong, LIANG Junfa, ZHANG Binbin, LIN Jiajian, PENG Cheng, ZENG Chengzhu, HE Liyuan. Evaluation and Analysis of the Colloidal Gold Immunochromatography Kits for the Rapid Detection of Pesticide Residue[J]. Science and Technology of Food Industry, 2021, 42(4): 187-193. DOI: 10.13386/j.issn1002-0306.2020040355

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Evaluation and Analysis of the Colloidal Gold Immunochromatography Kits for the Rapid Detection of Pesticide Residue

1. Guangzhou Institute of Food Inspection, Guangzhou 511405, China;
2. Guangdong Institute of Analysis, Guangzhou 510070, China

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Received Date: May 05, 2020
Available Online: March 01, 2021

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Abstract

Abstract

In this work,a quality evaluation method for rapid detection kits was established. The blind samples were prepared by spiking blank matrices and the homogeneity and the stability of them were verified simultaneously. Three different batches of rapid detection kits were randomly selected from each product,and each one was used to detect 50 blind samples which contained at least 20 positive and 20 negative samples. The specificity and accuracy(false positive and false negative)and other parameters of 14 commercially available assay kits for the rapid screening of pesticide residue were evaluated with a total of 2100 blind samples. The results showed that,11 of 14 of rapid detection kits passed the evaluation with a pass rate of 78.6%. One product had a higher false positive rate and two had a higher false negative rate;all the products had good specificity.This method could scientifically and objectively evaluate the quality of rapid testing products,and then screen out the rapid testing products with stable and reliable quality,and would provide technical support for grassroots regulatory authorities in the supervision of fruit and vegetable products.
- pesticide residue,
- rapid detection,
- colloidal gold immunochromatography,
- evaluation

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