Optimization of Extraction Process of <i>Hericium erinaceus</i> Triterpenes and Its Antioxidant Activity

LI Yingdi; CAO Yan; XIA Qile; YANG Kai; MAO Rongliang

doi:10.13386/j.issn1002-0306.2020040354

Volume 42 Issue 22

Nov. 2021

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Science and Technology of Food Industry > 2021 > 42(22): 153-159. > DOI: 10.13386/j.issn1002-0306.2020040354

LI Yingdi, CAO Yan, XIA Qile, et al. Optimization of Extraction Process of Hericium erinaceus Triterpenes and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2021, 42(22): 153−159. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020040354.

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Optimization of Extraction Process of Hericium erinaceus Triterpenes and Its Antioxidant Activity

1.
Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2.
Zhejiang University of Technology, College of Food Science and Technology, Hangzhou 310014, China
3.
Changshan Haofeng Agricultural Discovery Co., Ltd., Changshan 324207, China

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Received Date: May 05, 2020
Available Online: September 16, 2021

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Abstract

Abstract

In order to determine the optimal extraction process parameters of Hericium erinaceus triterpenes, the experiment was designed by Box-Behnken response surface method. Ethanol concentration, extraction temperature and material-liquid ratio were taken as the influencing factors, Hericium erinaceus triterpene yields were the response value. Design expert 10.0.3 software was used to establish quadratic polynomial fitting, draw contour map and response surface map. The best extraction conditions were screened out and the antioxidant activity was determined. The results showed that the optimal extraction process conditions were: Ethanol concentration 60%, temperature 55 ℃, material-liquid ratio 1:15(g:mL). Under this condition, the verification test showed that the yield of Hericium erinaceus triterpenes was 0.28%, close to the predicted value, indicating that the model established by the response surface method was accurate and reliable, and could reasonably optimize the extraction process of Hericium erinaceus triterpenes. Through comprehensive evaluation of three antioxidant systems: DPPH·scavenging ability, reducing power, ABTS⁺· scavenging ability, as the concentration of the extract increased, there was a good dose relationship for free radical scavenging. The DPPH and ABTS⁺ free radical half-inhibitory concentrations(IC₅₀) were (6.375±0.020) mg·mL⁻¹ and (0.355±0.040) mg·mL⁻¹, respectively, and the triterpene of Hericium erinaceus had certain antioxidant properties.
- Hericium erinaceus,
- triterpenoids,
- extraction,
- response surface analysis,
- antioxidant activity

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References

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