Safety Evaluation of Jingshen Tablet and Its Anti-acute Alcoholic Liver Injury

Objective: This article evaluates the safety of Jingshen Tablets and studies its auxiliary protective function on acute alcoholic liver injury. Methods: Through the acute toxicity test in mice, the micronucleus test in mouse bone marrow polychromatic erythrocytes, the sperm deformity test in mice, the Ames test and the 30-day feeding test in rats, the safety and toxicological evaluation of Jingshen tablets were carried out.60 mice were randomly divided into 5 groups. The control group of the blank group was given sterile water, and the model control group and each dose group were given 50% ethanol to establish an alcoholic liver injury model. After successful model building, the Jingshen tablets were administered orally and administered at 5, 10, and 30 times(0.40, 0.80, 2.40 g·kg^-1) of the recommended human dose(4.8 g/60 kg·BW), and the scene was recorded and observed. The effect of ginseng tablets on the contents of malondialdehyde(MDA), reduced glutathione(GSH) and triglyceride(TG) in mouse liver tissue.Results: The maximum dose in the acute oral test in mice was all greater than 15 g/(kg·BW), and the acute toxicity was classified as non-toxic, the micronucleus rate of bone marrow polychromatic erythrocytes and the incidence of sperm abnormalities in each dose group of mice were no significant difference(P>0.05), compared with those in the solvent control group. In the Ames test, the number of reverted colonies in each dose group did not exceed the number of reverted colonies in the solvent control group and the untreated control group by more than 2 times, indicating that Jingshen tablets have no mutagenic effect, There was no significant difference in organ index, biochemical and blood indexes between rats fed for 30 days and solvent control group(P>0.05), compared with model control group, 2.40 g·(kg·BW) ^-1 group could significantly reduce liver tissue the content of malondialdehyde and triglyceride in the medium(P<0.05). The pathological results showed that all dose groups of Jingshen tablets could alleviate steatosis and inflammatory infiltration in liver cells. Conclusion: Jingshen tablets have the effect of anti-acute alcoholic liver injury, and are safe and non-toxic within the scope of the dose.