Rapid Detection of 30 Kinds of Anti Fatigue and Sleep Improving Drugs in Health Food by High Performance Liquid Chromatography Triple Quadrupole Composite Ion Trap Mass Spectrometry

GAN Ninglan; SUN Chunhua; ZHOU Wei; LIU Changyu

doi:10.13386/j.issn1002-0306.2020040277

Volume 42 Issue 5

Feb. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(5): 226-235. > DOI: 10.13386/j.issn1002-0306.2020040277

GAN Ninglan, SUN Chunhua, ZHOU Wei, LIU Changyu. Rapid Detection of 30 Kinds of Anti Fatigue and Sleep Improving Drugs in Health Food by High Performance Liquid Chromatography Triple Quadrupole Composite Ion Trap Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(5): 226-235. DOI: 10.13386/j.issn1002-0306.2020040277

Citation:

PDF (2820 KB)

Rapid Detection of 30 Kinds of Anti Fatigue and Sleep Improving Drugs in Health Food by High Performance Liquid Chromatography Triple Quadrupole Composite Ion Trap Mass Spectrometry

Jiangsu Institute for Product Quality Supervision and Inspection, Nanjing 210007, China

More Information

Received Date: April 23, 2020
Available Online: March 02, 2021

Graphical Abstract

Abstract

Abstract

Using high performance liquid chromatography triple quadrupole composite ion trap mass spectrometry(HPLC-QTRAP),a rapid detection method of 30 kinds of drugs for anti fatigue and sleep improving was established. Acetonitrile and 10 mmol/L ammonium acetate solution were used as mobile phase to elute in gradient at a flow rate of 250 μL/min,and then chemical separated by Phenomenex Kinetex C₁₈ column(50 mm×2.1 mm,2.6 μm).Through scheduled multiple reaction monitoring(Scheduled MRM)acquisition,simultaneous scan of positive and negative ions,information dependent acquisition(IDA)triggered enhanced product ion scan(EPI)mode the data was analyzed,and an EPI database was formed for qualitative confirmation.The results showed that the linearity of 30 compounds in capsule,tablet and granule was good,the coefficient of determination R² was more than 0.996,the detection limit(LOD)was 0.01~0.72 μg/kg,the precision(RSD)was less than 6%,the recovery of standard addition was 84.5%~104.7%,the mixed reference solution had good stability within 24 h. This method has the advantages of fast detection speed,large flux and high sensitivity. It is suitable for the determination of 30 kinds of illegal added drugs in the health food of relieving fatigue and improving sleep,and provides effective technical support for the health food supervision under the new situation.

FullText(HTML)

References (30)

References

[1]	夏子雲,吴美香.新时期我国保健食品的现状分析[J].食品安全导刊,2019(24):96.
[2]	武阳阳.保健食品的问题与治理[J].食品安全导刊,2018(10):42-43.
[3]	刘垠孜.我国保健食品的安全问题及应对策略研究[J].食品安全导刊,2018(6):34.
[4]	Dong Y C,Du S M,Chen Y S,et al. Types and harm of illegally added chemicalsin Chinese traditional patent medicine and health food as well as countermeasure against the phenomenon[J]. Lishizhen Med Mater Med Res,2014,17(8):1601-1602.
[5]	Erica L B,Anna T K,Jeffrey P W,et al. A case report of fatal desmethyl carbodenafil toxicity[J]. J Anal Toxicol,2017,41(3):250-255.
[6]	傅蓉,张鹏,刘芳,等.全自动固相萃取-紫外可见分光光度法测定保健食品中总皂苷含量[J].食品安全质量检测学报,2019,10(23):7835-7838.
[7]	张欣烨,张利锋,张洁,等.紫外分光光度法在保健食品检测中的应用[J].中国卫生工程学,2015,14(4):347-349.
[8]	叶静.保健食品及中成药中违禁药物检测方法研究[J].检验检疫学刊,2019,29(6):118-119.
[9]	李静辉,刘吉成,马德志,等.薄层色谱-表面增强拉曼光谱法快速检测改善睡眠类保健食品的主要成分及其光谱表征[J].中国食品卫生杂志,2018,30(4):376-382.
[10]	程燕,谭顺中,姜登军.超高效液相色谱法同时测定功能性饮品和抗疲劳类保健食品中非法添加的11种化学药物[J].食品安全质量检测学报,2018,9(15):4078-4084.
[11]	赵琪,贾昌平,钱叶飞,等.UPLC-DAD法快速检测保健食品中13种非法添加化学物质[J].中成药,2019,41(11):2792-2796.
[12]	Shah P A,Shah J V,Sanyal M,et al. LC-MS/MS analysis of metformin,saxagliptin and 5-hydroxy saxagliptin in human plasma and its pharmacokinetic study with a fixed-dose formulation in healthy Indian subjects[J]. Biomedical Chromatography,2017,31(3):c3809-c3819.
[13]	Bitsika V,Duveau V,Simon-areces J,et al. High-throughput LC-MS/MS proteomic analysis of a mouse model of mesiotemporal lobe epilepsy predicts microglial activation underlying disease development[J]. Journal of Proteome Research,2016,15(5):1546-1562.
[14]	国家食品药品监督管理局药品检验补充方法和检验项目批准件批准件编号2009024.安神类中成药[Z].2009-12-04.
[15]	国家食品药品监督管理局药品检验补充方法和检验项目批准件批准件编号2012004.安神类中成药和保健品[Z]. 2012-08-02.
[16]	国家食品药品监督管理局药品检验补充方法和检验项目批准件批准件编号2009030.补肾壮阳类中成药[Z].2009-12-07.
[17]	Strano-Rossia S,Odoardia S,Castrignanòa E,et al. Liquid chromatography-high resolution mass spectrometry(LC-HRMS)determination of stimulants,anorectic drugs and phosphodiesterase 5 inhibitors(PDE5I)in food supplements[J].Journal of Pharmaceutical and Biomedical Analysis,2014,106:144-152.
[18]	欧爱芬,张挺,彭述辉.胶体金免疫层析试纸条法和HPLC-QTOF-MS法检测保健品中那非类化学药物的比较[J]. 仲恺农业工程学院学报,2017,30(3):32-36.
[19]	Li K,Guo CC,Shi F,et al. Research progress in determination technologies for adulterated phosphodiesterase-5(PDE-5)inhibitors and their analogue in invigorant herbal medicines and dietary supplements[J]. Chin J Pharm Anal,2018,38(4):566-573.
[20]	马春艳,封淑华.保健酒及酒类制品中非法添加PDE-5抑制剂及其衍生物的监测及结果分析[J]. 药物分析杂志,2017,37(2):277-282.
[21]	Kee CL,Ge X,Gilard V,et al. A review of synthetic phosphodiesterase type 5 inhibitors(PDE-5i)found as adulterants in dietary supplements[J]. J Pharm Biomed Anal,2018,147(SI):163-170.
[22]	勾新磊,赵新颖,高峡,等.超高效液相色谱-串联质谱法测定保健食品中10种磷酸二酯酶-5抑制剂[J].质谱学报,2016,37(6):554-561.
[23]	Fu Y Q,Zhao C X,Lu X,et al. Nontargeted screening of chemical contaminants and illegal additives in food based on liquid chromatographyehigh resolution mass spectrometry[J]. Trends in Analytical Chemistry,2017,96:89-98.
[24]	贾昌平,张圆,陈丽波,等.安神类保健食品中非法添加化学物质的UPLC-ESI-Q-TOF/MS分析[J].中国药师,2015,18(7):1129-1132 ,1135.
[25]	丁博,王志元,谢建军,等.QuEChERS前处理技术联合液相色谱-四极杆飞行时间质谱法检测保健食品24种违禁降血糖、降血压和降血脂药物[J].色谱,2016,34(6):583-590.
[26]	姜树银,郭常川,石峰,等.Q-Orbitrap高分辨质谱用于降血脂类中成药和保健食品中非法添加物的快速筛查、鉴定和定量[J].药物分析杂志,2015,35(8):1447-1452.
[27]	杜彦山,李强,吴春敏,等.超高效液相色谱-四极杆/静电场轨道阱高分辨质谱快速筛查及定量分析保健品中11种非法添加降糖药物[J].色谱,2015,33(4):371-376.
[28]	于泓,胡青,孙健,等.超高效液相色谱-四极杆-飞行时间质谱法定性筛查保健食品中西地那非及其相关功效类非法添加化合物[J].色谱,2018,36(10):1005-1017.
[29]	洪灯,谢文,侯建波,等.高效液相色谱-四极杆/静电场轨道阱高分辨质谱法快速筛查保健食品中的西布曲明及其5种衍生物[J].色谱,2019,37(11):1173-1178.
[30]	徐文峰,金鹏飞,徐硕,等.超高效液相色谱-四极杆串联飞行时间质谱定性定量检测保健食品及中成药中非法添加的25种降压类化合物[J].药物分析杂志,2019,39(7):1295-1301.

[1]	FENG Lifeng, HUANG Qian, YE Mengwei, HUANG Yonghui, HUANG Yan, LIN Haoxue, LIANG Min, XU Hui. Determination of 30 Kinds of Antiparasitic Drugs in Animal-derived Foods by QuEChERS-UPLC-MS/MS[J]. Science and Technology of Food Industry, 2024, 45(6): 280-288. DOI: 10.13386/j.issn1002-0306.2023050175
[2]	WU Yanlei, SU Min, ZHOU Chunjie, TIAN Yuan, WANG Min. Simultaneous Determination of 76 Veterinary Drugs and Their Metabolites in Pork by QuEChERS-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2023, 44(20): 311-321. DOI: 10.13386/j.issn1002-0306.2022110330
[3]	YU Ling, SONG Lihua. Rapid Determination of Five Alkaloids in Catering Crayfish by QuEChERS-UPLC-MS/MS[J]. Science and Technology of Food Industry, 2023, 44(2): 324-331. DOI: 10.13386/j.issn1002-0306.2022030219
[4]	XU Xing, ZHANG Yan, SHU Ping, YANG Weihua. Determination of 20 Kinds of Cephalosporins Residues in Animal Derived Food by QuEChERS-LC-MS/MS with Isotope Internal Standards Method[J]. Science and Technology of Food Industry, 2022, 43(24): 320-330. DOI: 10.13386/j.issn1002-0306.2022030194
[5]	WANG Jing, ZHANG Haichao, JIA Haitao, AI Lianfeng. Determination of 14 Anabolic Androgenic Steroids in Animal Derived Foods by QuEChERS-High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2022, 43(20): 291-299. DOI: 10.13386/j.issn1002-0306.2021120163
[6]	TAN Gaohao, WANG Chengye, ZHENG Jinming, YIN Qingchun, XU Bin, WU Jiren. Simultaneous Determination of 37 Veterinary Drugs in Animal-Derived Foods by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry（UPLC-MS/MS）[J]. Science and Technology of Food Industry, 2021, 42(17): 225-234. DOI: 10.13386/j.issn1002-0306.202009030
[7]	ZHOU Jia, SU A-long, ZHU Shu-qiang, WANG Li-jun, YU Jie. Determination of Insecticide of Phenylpyrazole Derivatives in Poultry Derived Foods by QuEChERS Method Combined with Liquid Chromatography-Mass Spectrometry[J]. Science and Technology of Food Industry, 2019, 40(16): 206-211,217. DOI: 10.13386/j.issn1002-0306.2019.16.035
[8]	YANG Lu-ping, SHAO Li-jun, WANG Guo-ling, WANG Xi-ning, REN Xiao-fei, LIU Yong-jun. Determination of Thirteen Pesticides in Edible Mushrooms by QuEChERS with UPLC MS/MS[J]. Science and Technology of Food Industry, 2019, 40(14): 247-253. DOI: 10.13386/j.issn1002-0306.2019.14.040
[9]	GAO Hai-rong. Determination of Nitromidazoles in Fish by QuEChERS Extraction with ZrO₂Coupled to High Performance Liquid Chromatography-tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2019, 40(4): 266-270. DOI: 10.13386/j.issn1002-0306.2019.04.044
[10]	WANG Ke-yu, YANG Hong-xu, WANG Chao-ying, RUAN Hua-ping, CHEN Dong-po, ZHOU Peng. Application of Baking,Boiling,Microwaving Heating Techniques in Animal-derived Food Processing[J]. Science and Technology of Food Industry, 2018, 39(13): 325-330. DOI: 10.13386/j.issn1002-0306.2018.13.060

Supplements (0)

Cited By

Cited by

Periodical cited type(18)

1.	温雅君，王全红，杨红菊，孙志伟，刘希艳，高利文，肖志勇. 胶体金免疫层析法快速检测韭菜中腐霉利的质量分析与评价. 农药科学与管理. 2025(01): 29-33 .
2.	杨静，方芳，沈媛，孙娟，吴仑，陈翔，贾晨，李英. 水产品中地西泮快速检测产品技术评价. 食品安全质量检测学报. 2025(07): 102-108 .
3.	姚南南，刘芳，高会群，张学龙，杜斌，张郢，杨梅，蒲小容. 基于荧光微球的多菌灵残留快速检测试纸条的研制. 食品科技. 2024(01): 344-350 .
4.	陈喆，高文分，刘屹. 快速显色法筛查祛斑美白类化妆品中糖皮质激素. 化学分析计量. 2024(07): 7-11+22 .
5.	薛芳，张照红，殷慧龄. 胶体金免疫层析法在农残快速检测中的研究与探讨. 新疆农业科技. 2024(06): 39-41 .
6.	骆丽清，伍浚铧，胡茗淇，黎喜萍. 氟虫腈和水胺硫磷胶体金试剂盒性能考察与分析. 食品安全导刊. 2024(35): 72-75 .
7.	杨睿，蔡琳，卢灿鑫，李乐诗，张洁吟，刘晓晗，王韦达. 致病菌测试片质量评价方法研究. 食品安全质量检测学报. 2024(23): 41-51 .
8.	陈振东. 食品安全快速检测技术在食品安全监督中的运用浅析. 食品安全导刊. 2023(03): 121-123 .
9.	王元清，周巧，李莎，韩静，王惠，李建龙，何利，陈姝娟，刘爱平，李琴，胡凯弟，刘书亮. 市售原粮农药残留快速检测产品的质量评价与分析. 中国粮油学报. 2023(04): 122-128 .
10.	刘海虹，刘耀慧，雷毅. 基于真实食品的兽药残留快检结果准确性验证及应用探索. 食品安全导刊. 2023(33): 63-67 .
11.	罗俊霞，张刚，申战宾，杨华，叶茂，段鹿梅，李艳珍，赵建波，桑丽雅，马蕾，张威. 胶体金免疫层析技术应用于农药残留检测的研究进展. 农产品质量与安全. 2022(01): 41-49 .
12.	倪诗瑶，刘欠欠. 草莓农药残留快速检测方法对比分析. 上海农业科技. 2022(01): 30-32 .
13.	顾晔，张爽，王成军，李悦，杨雨柔. 基于免疫原理的7种磺胺类兽药残留快速检测试剂结果准确性评估. 食品安全质量检测学报. 2022(03): 992-1000 .
14.	陈振东. 食品安全检测技术在保障食品质量安全中的作用. 食品安全导刊. 2022(35): 166-168 .
15.	叶秋雄，毛新武，梁俊发，张彬彬，林嘉健，彭程，易云婷. 农贸市场食用农产品快速检测工作监督评价与效果分析. 食品安全质量检测学报. 2021(19): 7826-7830 .
16.	泮秋立，胡明燕，沈祥震，孙嵛林，李峥，王骏. 食用农产品批发市场自建快检室运行中存在的问题及建议. 食品安全导刊. 2021(29): 7-10 .
17.	占绣萍，刘彬，黄兰淇，陈秀，马琳，陈建波，赵莉. 应用胶体金法检测叶类蔬菜中吡虫啉、多菌灵、啶虫脒、噻虫嗪的残留量分析. 农药科学与管理. 2021(10): 24-31 .
18.	岳绪辉，杜斌，林栋，令狐克勇，杨梅，付秋平，李丙凤，杨曦. 草甘膦胶体金免疫层析试纸条的研制. 食品科技. 2021(12): 301-307 .

Other cited types(1)

Get Citation

PDF

XML

Article Metrics

Article views (201) PDF downloads (14) Cited by(19)

Science and Technology of Food Industry

Rapid Detection of 30 Kinds of Anti Fatigue and Sleep Improving Drugs in Health Food by High Performance Liquid Chromatography Triple Quadrupole Composite Ion Trap Mass Spectrometry