Research Progress of Digital PCR Technology and Its Application in Food Detection

LIU Ke; LI Mengxia; ZHANG Liang; KANG Dacheng; HU Xiaojie; LIU Yunguo

doi:10.13386/j.issn1002-0306.2020040194

Volume 42 Issue 4

Feb. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(4): 319-324. > DOI: 10.13386/j.issn1002-0306.2020040194

LIU Ke, LI Mengxia, ZHANG Liang, KANG Dacheng, HU Xiaojie, LIU Yunguo. Research Progress of Digital PCR Technology and Its Application in Food Detection[J]. Science and Technology of Food Industry, 2021, 42(4): 319-324. DOI: 10.13386/j.issn1002-0306.2020040194

Citation:

PDF (1131 KB)

Research Progress of Digital PCR Technology and Its Application in Food Detection

1. School of Food Science and Technology, Xinjiang University, Urumqi 830046, China;
2. School of Life Sciences, Linyi University, Linyi 276000, China;
3. Dongying District Bureau of Science and Technology, Dongying 257000, China;
4. Linyi Xiaolu Biotechnology Co., Ltd., Linyi 276000, China

More Information

Received Date: April 16, 2020
Available Online: March 01, 2021

Graphical Abstract

Abstract

Abstract

Digital PCR(dPCR)technology is a rapid development of absolute quantification of nucleic acid molecules in recent years. It has the advantages of quantification without standard curve,high sensitivity and high accuracy. With the increasing requirements of detection technology in various fields,the application of digital PCR technology in the analysis of genetically modified products,animal and plants products,microorganisms and other fields has become more and more widespread. This article reviews the principles and applications of dPCR,analyzes its problems and prospects,and provides a reference for the application of digital PCR.
- digital PCR,
- genetically modified products,
- derived ingredients,
- microorganism

FullText(HTML)

References (59)

References

[1]	Morisset D,Štebih D,Milavec M,et al. Quantitative analysis of food and feed samples with droplet digital PCR[J]. PloS One,2013,8(5):e62583.
[2]	Deng Tingting,Huang Wensheng,Ren Junan,et al. Verification and applicability of endogenous reference genes for quantifying GM rice by digital PCR[J]. Analytical Biochemistry,2019,587:113442.
[3]	于晓帆,高宏伟,孙敏,等. 荧光PCR和数字PCR法检测转基因DAS-44406-6品系大豆[J]. 食品科学,2016,37(16):235-241.
[4]	Cai Yicun,He Yuping,Lv Rong,et al. Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR[J]. PloS One,2017,12(8):e0181949.
[5]	Shehata H R,Li J,Chen S,et al. Droplet digital polymerase chain reaction(ddPCR)assays integrated with an internal control for quantification of bovine,porcine,chicken and turkey species in food and feed[J]. PloS One,2017,12(8):e0182872.
[6]	刘立兵,石蕊寒,项佳林,等. 应用微滴式数字聚合酶链式反应定量检测牛肉制品中的猪源性成分[J]. 肉类研究,2018,32(9):29-34.
[7]	赵丽青,方佩佩,唐静,等. 数字PCR定量检测食品中单核细胞增生李斯特氏菌方法的研究[J]. 食品安全质量检测学报,2017,8(11):4133-4138.
[8]	程逸宇,冯秋实,吴海晶,等. 微滴数字PCR定量检测乳制品中单核细胞增生李斯特氏菌[J]. 中国乳品工业,2019,47(12):37-40.
[9]	Sykes P J,Neoh S H,Brisco M J,et al. Quantitation of targets for PCR by use of limiting dilution[J]. Bio Techniques,1992,13(3):444-449.
[10]	Vogelstein B,Kinzler K W. Digital PCR[J]. Proceedings of the National Academy of Sciences,1999,96(16):9236-9241.
[11]	Hindson C M,Chevillet J R,Briggs H A,et al. Absolute quantification by droplet digital PCR versus analog real-time PCR[J]. Nature Methods,2013,10(10):1003-1005.
[12]	原霖,董浩,倪建强,等. 非洲猪瘟病毒微滴数字PCR检测方法的建立[J]. 畜牧与兽医,2019,51(7):81-84.
[13]	姚丽锋,王小玉,蔡教英,等. 贝类中GⅡ型诺如病毒的微滴式数字PCR定量检测方法[J]. 农产品质量与安全,2019,(4):20-25.
[14]	陈嘉茵,方苓,吴诗微,等. 一步法微滴数字PCR检测生菜中GⅡ型诺如病毒[J]. 食品科学,2019,40(4):332-337.
[15]	Tomoaki K,Moriya I,Hidetaka Y,et al. Application of digital PCR with chip-in-a-tube format to analyze Adenomatous polyposis coli(APC)somatic mosaicism[J]. Clinica Chimica Acta;International Journal of Clinical Chemistry,2017,475:91-96.
[16]	Dong Lianhua,Wang Xia,Wang Shangjun,et al. Interlaboratory assessment of droplet digital PCR for quantification of BRAF V600E mutation using a novel DNA reference material[J]. Talanta,2020,207:120293.
[17]	朱慧,王哲颖,丁小青,等. 微滴式数字PCR技术检测外泌体PML-RARA融合基因的表达[J]. 中国实验血液学杂志,2019,27(3):747-752.
[18]	Yan Yong,Jia Xiaojun,Wang Henghui,et al. Dynamic quantification of avian influenza H7N9(A)virus in a human infection during clinical treatment using droplet digital PCR[J]. Journal of Virological Methods,2016,234:22-27.
[19]	吴衍恒,林金思,师舞阳,等. 应用芯片式数字PCR检测人血清中登革病毒载量[J]. 公共卫生与预防医学,2019,30(3):16-20.
[20]	刘平,余玲玲,王金丹,等. 数字PCR检测血浆游离DNA方法的建立及在结直肠癌中的初步应用[J]. 医学研究杂志,2018,47(6):38-44.
[21]	Staley Z R,Boyd R J,Shum P,et al. Microbial source tracking using quantitative and digital PCR to identify sources of fecal contamination in stormwater,river water,and beach water in a Great Lakes Area of Concern[J]. Applied and Environmental Microbiology,2018,84(20):e01634-18.
[22]	Shah P,Wang Zhiwu. Using digital polymerase chain reaction to characterize microbial communities in wetland mesocosm soils under different vegetation and seasonal nutrient loadings[J]. The Science of the Total Environment,2019,689:269-277.
[23]	Nyaruaba R,Mwaliko C,Kering K K,et al. Droplet digital PCR applications in the tuberculosis world[J]. Tuberculosis,2019,117:85-92.
[24]	朱鹏宇,张亮亮,杜智欣,等. 双重数字PCR在转基因水稻检测中的应用[J]. 植物检疫,2018,32(4):52-56.
[25]	缪青梅,汪小福,陈笑芸,等. 基于双重微滴数字PCR精准定量转基因水稻G6H1的方法研究[J]. 农业生物技术学报,2019,27(1):159-169.
[26]	蔡教英,姚丽锋,王小玉,等. 基于双重微滴式数字PCR对转基因油菜RF1品系的定量方法[J]. 现代食品科技,2018,34(6):282-287 ,173.
[27]	Gao Hongwei,Yu Xiaofan,Deng Tingting,et al. Event-specific detection of transgenic potato AV43-6-G7 using real-time and digital PCR methods[J]. BMC Biotechnology,16(1):74.
[28]	柴成梁,唐柏飞,常晓娇,等. 利用微滴式数字PCR技术检测大豆毛油中的转基因成分[J]. 中国粮油学报,2019,34(2):107-111 ,117.
[29]	Wang Xiaofu,Tang Ting,Miao Qingmei,et al. Detection of transgenic rice line TT51-1 in processed foods using conventional PCR,real-time PCR,and droplet digital PCR[J]. Food Control,2018,98:380-388.
[30]	Bogožalec K A,Demšar T,Štebih D et al. Digital PCR as an effective tool for GMO quantification in complex matrices[J]. Food Chemistry,2019,294:73-78.
[31]	Cottenet G,Blancpain C,Chuah P F. Performance assessment of digital PCR for the quantification of GM-maize and GM-soya events[J]. Anal Bioanal Chem,2019,411:2461-2469.
[32]	梁文,杨镇州,李妍,等. 转基因玉米MON89034、MON810、MIR162双重数字PCR定量方法的建立[J]. 中国测试,2019,45(6):70-76.
[33]	Bogožalec K A,Spilsberg B,Holst-Jensen A et al. Development and inter-laboratory assessment of droplet digital PCR assays for multiplex quantification of 15 genetically modified soybean lines[J]. Scientific Reports,2017,7(1):415-437.
[34]	华再东,刘圣财,肖伟,等. 转基因动物安全评价浅谈[J].湖北畜牧兽医,2019,40(9):10-14.
[35]	姜羽,胡佳莹,杨立桃.利用微滴数字PCR分析转基因生物外源基因拷贝数[J]. 农业生物技术学报,2014,22(10):1298-1305.
[36]	兰雪,张斯童,李哲,等. 微滴数字PCR技术在多拷贝木聚糖酶酿酒酵母工程菌筛选中的应用[J]. 食品科学,2018,39(10):179-184.
[37]	Nicolai K. Engineered Microorganisms for the Production of food additives approved by the European Union-A systematic analysis[J]. Frontiers in Microbiology,2018,9:1746.
[38]	Paracchini V,Petrillo M,Reiting R,et al. Molecular characterization of an unauthorized genetically modified Bacillus subtilis production strain identified in a vitamin B₂ feed additive[J]. Food Chemistry,2017,230:681-689.
[39]	Deckers M,Vanneste K,Winand R,et al. Strategy for the identification of micro-organisms producing food and feed products:Bacteria producing food enzymes as study case[J]. Food Chemistry,2020,305:125431.
[40]	Fraiture M,Bogaerts B,Winand R,et al. Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing[J]. Scientific Reports,2020,10(11):2-7.
[41]	李丹,王守伟,臧明伍,等. 国内外经济利益驱动型食品掺假防控体系研究进展[J]. 食品科学,2018,39(1):320-325.
[42]	李伟琦,刘津,李志勇,等. 火鸡源性成分数字PCR通用定量检测方法的研究[J]. 黑龙江大学自然科学学报,2018,35(3):309-316.
[43]	陈晨,张岩,李永波,等. 微滴式数字PCR对肉制品中羊肉和猪肉定量分析[J]. 现代食品科技,2018,34(1):221-226 ,194.
[44]	Wang Qiang,Cai Yicun,He Y,et al. Droplet digital PCR(ddPCR)method for the detection and quantification of goat and sheep derivatives in commercial meat products[J]. European Food Research and Technology,2018,244(4):767-774.
[45]	Noh E S,Park Y J,Kim E M,et al. Quantitative analysis of Alaska pollock in seafood products by droplet digital PCR[J]. Food Chemistry,2019,275:638-643.
[46]	刘二龙,卢丽,凌莉,等. 红薯源性成分微滴式数字PCR的检测与定量分析[J]. 现代食品科技,2019,35(7):273-277 ,285.
[47]	郭楠楠,张岩,李永波,等. 微滴式数字PCR定量检测杏仁露中杏仁、花生源性成分[J]. 食品科学,2019,40(14):346-351.
[48]	任君安,邓婷婷,黄文胜,等. 微滴式数字聚合酶链式反应精准定量检测羊肉中掺杂猪肉[J]. 食品科学,2017,38(2):311-316.
[49]	Ren Junan,Deng Tingting,Huang Wensheng,et al. A digital PCR method for identifying and quantifying adulteration of meat species in raw and processed food[J]. PloS One,2017,12(3):e0173567.
[50]	Scollo F,Egea L A,Gentile A,et al. Absolute quantification of olive oil DNA by droplet digital-PCR(ddPCR):Comparison of isolation and amplification methodologies[J]. Food Chemistry,2016,213:388-394.
[51]	Cao Weiwei,Li Yiming,Chen Xun,et al. Species identification and quantification of silver pomfret using the droplet digital PCR assay[J]. Food Chemistry,2020,302.
[52]	赵新,兰青阔,陈锐,等. 应用微滴数字PCR技术快速检测食用菌中沙门氏菌[J]. 食品与生物技术学报,2017,36(3):315-321.
[53]	Wang Meng,Yang Junjie,Gai Zhongtao,et al. Comparison between digital PCR and real-time PCR in detection of Salmonella typhimurium in milk[J]. International Journal of Food Microbiology,266:251-256.
[54]	周巍,李月华,孙勇,等. 微滴式数字PCR技术定量检测发酵乳中金黄色葡萄球菌[J]. 食品科学,2017,38(16):287-291.
[55]	魏咏新,马丹,李丹,等. 食品中Escherichia coli O157:H7微滴数字PCR绝对定量检测方法的建立[J/OL]. 食品科学:1-13[2020-01-11 ]. http://kns.cnki.net/kcms/detail/11.2206.TS.20190530.1145.036.html.
[56]	Hansen S J Z,Morovic W,Demeules M,et al. Absolute enumeration of probiotic strains Lactobacillus acidophilus NCFM^® and Bifidobacterium animalis subsp. lactis Bl-04^® via chip-based digital PCR[J]. Frontiers in Microbiology,2018,9:704.
[57]	Gobert G,Cotillard A,Fourmestraux C,et al. Droplet digital PCR improves absolute quantification of viable lactic acid bacteria in faecal samples[J]. Journal of Microbiological Methods,2018,148:64-73.
[58]	Cui Xueyan,Jin Maojun,Zhang Chan,et al. Enhancing the sensitivity of the bio-barcode immunoassay for triazophos detection based on nanoparticles and droplet digital polymerase chain reaction[J]. Journal of Agricultural and Food Chemistry,2019,67(46):12936-12944.
[59]	Whale A S,Huggett J F,Tzonev S. Fundamentals of multiplexing with digital PCR[J]. Biomolecular Detection and Quantification,2016,10:15-23.

Supplements (0)

Cited By

Cited by

Periodical cited type(4)

1.	柏倩，毛绍云，徐倩. 混菌发酵女贞子制备抗氧化肽及其结构的鉴定. 中国食品添加剂. 2025(02): 34-42 .
2.	潘立妮，周家华，潘雯，徐冉冉，王云香，李洪雯，王宝刚，赵山山. 樱桃果酒发酵动力学及抗氧化能力研究. 中国酿造. 2024(10): 199-204 .
3.	邹平，徐莹，陈文涛，张迎阳，徐荣. 牡丹籽粕中黄酮类化合物的提取工艺优化及膜法分离纯化. 食品工业科技. 2023(18): 258-267 . 本站查看
4.	王颖，王涛，马利华. 膨化技术对燕麦酵素粉营养性及抗氧化性的影响. 保鲜与加工. 2023(11): 31-36 .