Process Optimization of Protein Extraction from <i>Moringa oleifera</i> Leaf by Enzymatic Hydrolysis

GUO Gangjun; HU Xiaojing; XU Rong; MA Shangxuan; ZHANG Zubing; LI Haiquan

doi:10.13386/j.issn1002-0306.2020040110

Volume 42 Issue 5

Feb. 2021

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Science and Technology of Food Industry > 2021 > 42(5): 194-199. > DOI: 10.13386/j.issn1002-0306.2020040110

GUO Gangjun, HU Xiaojing, XU Rong, MA Shangxuan, ZHANG Zubing, LI Haiquan. Process Optimization of Protein Extraction from Moringa oleifera Leaf by Enzymatic Hydrolysis[J]. Science and Technology of Food Industry, 2021, 42(5): 194-199. DOI: 10.13386/j.issn1002-0306.2020040110

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Process Optimization of Protein Extraction from Moringa oleifera Leaf by Enzymatic Hydrolysis

1. Yunnan Institute of Tropical Crops, Jinghong 666100, China;
2. School of Chemistry and Engineering, Wenshan University, Wenshan 663000, China

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Received Date: April 12, 2020
Available Online: March 02, 2021

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Abstract

Abstract

In order to optimize the extraction process of cellulase and pectinase hydrolysis to extract protein from Moringa oleifera leaf,the effects of five processing conditions on the extraction rate were explored by single factor and orthogonal array design methods. Results showed that the effect of five processing conditions on the extraction rate of Moringa oleifera leaf protein with cellulase was in the descending order of reaction temperature,substrate concentration,pH,hydrolysis time and enzyme dosage. The optimal hydrolysis conditions were found to be reaction at 50 ℃ and pH5.0 for 70 min with a substrate concentration of 7.0 g/L and an enzyme dosage of 800 U/g,the extraction rate reached 43.85%. In the case of hydrolysis with pectinase,the decreasing order of five processing conditions was enzyme dosage,substrate concentration,hydrolysis time,temperature,and pH,and their optimal levels which provided maximum extraction rate as high as 32.26% were determined as 1400 U/L,9.0 g/L,50 min,50 ℃,and 4.0,respectively. In both cases,the effect of five processing conditions on the extraction rate reached up to a significant level(P<0.01). Under the optimized hydrolysis conditions,cellulase was superior to pectinase in extracting proteins from Moringa oleifera leaf.
- Moringa oleifera leaf,
- cellulose,
- pectinase,
- protein,
- extraction rate

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[1]	刘昌芬,李国华.辣木的营养价值[J].热带农业科技,2004,27(1):4-7 ,29.
[2]	Prabakaran M,Kim S H,Sasireka A,et al. Polyphenol composition and antimicrobial activity of various solvent extracts from different plant parts of Moringa oleifera[J].Food Bioscience,2018,26:23-29.
[3]	罗晓波,汪开毓,吉莉莉,等.辣木叶的价值及其开发利用研究进展[J].资源开发与市场,2016,32(11):1362-1366 ,1375.
[4]	刘昌芬.神奇保健植物辣木及其栽培技术[M].昆明:云南科技出版社,2013:1.
[5]	Oyeyinka A T,Oyeyinka S A.Moringa oleifera as a food fortificant:Recent trends and prospects[J].Journal of the Saudi Society of Agricultural Sciences,2018,17(2):127-136.
[6]	郭刚军,龙继明,黄艳丽,等.多油辣木不同部位营养成分分析及评价[J].食品工业科技,2016,37(22):354-358 ,364.
[7]	Ziani B E C,Rached W,Bachari K,et al. Detailed chemical composition and functional properties of Ammodaucus leucotrichus Cross.&Dur. and Moringa oleifera Lamarck[J].Journal of Functional Foods,2019,53:237-247.
[8]	李东,赵一鹤.辣木研究现状及发展趋势[J].西部林业科学,2018,47(5):32-38.
[9]	Balusamy S R,Perumalsamy H,Ranjan A,et al. A dietary vegetable,Moringa oleifera leaves(drumstick tree)induced fat cell apoptosis by inhibiting adipogenesis in 3T3-L1 adipocytes[J].Journal of Functional Foods,2019,59:251-260.
[10]	Gopalakrishnan L,Doriya K,Kumar D S.Moringa oleifera:A review on nutritive importance and its medicinal application[J].Food Science and Human Wellness,2016,5(2):49-56.
[11]	刘凤霞,王苗苗,赵有为,等.辣木中功能性成分提取及产品开发的研究进展[J].食品科学,2015,36(19):282-286.
[12]	盛军. 现代辣木生物学[M]. 昆明:云南科技出版社,2015:186.
[13]	段琼芬,李迅,陈思多,等.辣木营养价值的开发利用[J].安徽农业科学,2008,36(29):12670-12672.
[14]	郭刚军,胡小静,徐荣,等.干燥方式对辣木叶营养、功能成分及氨基酸组成的影响[J].食品科学,2018,39(11):39-45.
[15]	奚海燕,张晖,姚惠源.碱酶分步法从米粉中提取大米蛋白工艺的研究[J].粮油食品科技,2007,15(6):12-14.
[16]	熊瑶. 辣木叶蛋白质的提取及其饮品研制[D]. 福州:福建农林科技大学,2012:7.
[17]	李月,钟惠萍,陈晓雯,等.辣木活性成分、提取工艺及应用研究进展[J].内蒙古民族大学学报(自然科学版),2019,34(5):369-375.
[18]	毕双同,蓝海军.酶水解法制备芝麻蛋白工艺条件优化[J].安徽农业科学,2018,46(21):166-168 ,192.
[19]	李圆圆. 茶渣蛋白的酶法提取及功能性质研究[D]. 无锡:江南大学,2013:16-20.
[20]	陈汝财.辣木蛋白超声辅助提取试验[J].福建农业科技,2015(10):31-34.
[21]	吴晓红,郑月明,付兆龙,等.酶法提取红松种子蛋白工艺的研究[J].中国粮油学报,2009,24(9):144-148.
[22]	Wang M,Jiang L Z,Li Y,et al. Optimization of extraction process of protein isolate from mung bean[J].Procedia Engineering,2011,15:5250-5258.
[23]	崔淼,凌孟硕,孙明奎,等.碱酶两步法提取沙棘籽蛋白的工艺研究[J].中国油脂,2012,37(9):31-35.
[24]	朱天明,陈泠伶,杨潇,等.纤维素酶辅助提取桑叶中叶蛋白的工艺[J].西华大学学报(自然科学版),2016,35(2):77-81,109.
[25]	郭刚军,邹建云,胡小静,等.液压压榨澳洲坚果粕酶解制备多肽工艺优化[J].食品科学,2016,37(17):173-178.
[26]	Cui Q Y,Ni X H,Zeng L,et al. Optimization of protein extraction and decoloration conditions for tea residues[J]. Horticultural Plant Journal,2017,3(4):172-176.
[27]	董娟,赵巧玲,刘扬铭,等.酶辅助法提取脱脂葡萄籽蛋白的工艺研究[J].粮食与油脂,2017,30(12):20-24.

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Process Optimization of Protein Extraction from Moringa oleifera Leaf by Enzymatic Hydrolysis