Screening of Phospholytic Bacterium Combination WF542 and Combination of Multi-bacterial Agents on Growth of Pepper

The combination of phosphate-dissolving bacteria was screened to study the effect of combined multi-agents on the growth of pepper. 5 phosphate solubilizing bacteria(HSYQ18,SYC3,WS54、FWG2,SCPG-7)preserved in the laboratory were identified by morphological characteristics,physiological and biochemical analysis,and 16S rRNA sequence analysis,screening for phosphate-dissolving bacteria combinations,characteristics of responsing to environmental factors,solubilizing phosphorus,secreting ferritin and indole acetic acid(IAA)were tested. The results showed that HSYQ18 was Serratia rubidaea,SYC3 belonged to Pantoaea dispersa,and WS54,FWG2 and SCPG-7 were Pseudomonas. Phospholytic bacteria combination WF542 had the ability to secrete ferritin and IAA. The phosphate-solubilizing bacteria combination WF542 was cultured in Pikovskaya inorganic phosphorus(PKO)medium for 96 h and the inorganic phosphorus concentration reached 530 mg/L. The optimum growth temperature of WF542 was 35 ℃,the combination WF542 could still grow at low temperature,and high temperature slowly. The combination WF542 could grow at pH5.5~8.0,the combination WF542 grew at concentration of NaCl 1%~7%,while had little growth when the concentration was over 9%.The combination of nitrogen-fixing bacteria and WF542 could effectively promote the growth of pepper seedlings. The combination of nitrogen-fixing bacteria and phosphorus-dissolving bacteria WF542 could be used as microbial fertilizer in agricultural production,which could provide an important basis for the utilization of excellent phosphate-dissolving bacteria resources and green production of vegetables.