LIU Rui, LIU Xinran, ZHU Na, HAO Yuntao, KANG Jiawei, MAO Ruixue, HOU Chao, ZHANG Li, YANG Jiao, LI Yong. Preventive Effect of Walnut Oligopeptide on Alcoholic Gastric Ulcer in Rats[J]. Science and Technology of Food Industry, 2021, 42(2): 316-320. DOI: 10.13386/j.issn1002-0306.2020030398
Citation: LIU Rui, LIU Xinran, ZHU Na, HAO Yuntao, KANG Jiawei, MAO Ruixue, HOU Chao, ZHANG Li, YANG Jiao, LI Yong. Preventive Effect of Walnut Oligopeptide on Alcoholic Gastric Ulcer in Rats[J]. Science and Technology of Food Industry, 2021, 42(2): 316-320. DOI: 10.13386/j.issn1002-0306.2020030398

Preventive Effect of Walnut Oligopeptide on Alcoholic Gastric Ulcer in Rats

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  • Received Date: March 29, 2020
  • Available Online: January 20, 2021
  • Objective:This study explored the protective effect of walnut oligopeptides(WOPs)on alcoholic gastric ulcer and its possible mechanism by establishing a model of acute ethanol-induced gastric ulcer in rats. Methods:Male SD rats were randomly divided into 7 dose groups(n=10):Normal control group,model control group,omeprazole control group(20 mg/kg),WOPs low,medium and high dose groups(220,440,880 mg/kg,respectively). After oral gavage for 30 consecutive days,rats were given 5 mL/kg absolute ethanol to induce gastric ulcer. Then the levels of gastric ulcer index,hematological parameters,prostaglandin E2(PGE2),nitric oxide(NO)and myeloperoxidase(MPO)of gastric tissues in rats were evaluated. Results:The gastric ulcer index of the WOPs low,medium and high dose groups was significantly lower than that of the model control group(P<0.05),and the gastric ulcer index of the WOPs high dose group was lower than that of the omeprazole group(P<0.05). WOPs had a significant inhibitory effect on the abnormal increase of white blood cell count and monocyte percentage in model rats(P<0.05). In addition,WOPs could significantly increase the content of PGE2 and reduce the levels of MPO and NO(P<0.05). Conclusion:The WOPs would have protective effect on alcoholic gastric ulcer,and the probably mechanism of its action was related to reducing the severity of gastric ulcer,increasing the contents of PGE2,reducing the aggregation and infiltration of neutrophils and the pathological increasing of nitric oxide contents.
  • [1]
    WHO. Global status report on alcohol and health 2018. Geneva:World Health Organization.(https://www.who.int/substance_abuse/publications/global_alcohol_report/en/).
    [2]
    Raish M,Ahmad A,Ansari M A,et al. Momordica charantia polysaccharides ameliorate oxidative stress,inflammation,and apoptosis in ethanol-induced gastritis in mucosa through NF-kB signaling pathway inhibition[J]. International Journal of Biological Macromolecules,2018,111:193-199.
    [3]
    张亭,杜倩,李勇. 核桃的营养成分及其保健功能的研究进展[J]. 中国食物与营养,2018,24(7):64-69.
    [4]
    Ma S,Huang D,Zhai M,et al. Isolation of a novel bio-peptide from walnut residual protein inducing apoptosis and autophagy on cancer cells[J]. BMC Complementary and Alternative Medicine,2015,15(15):413.
    [5]
    Chen N,Yang H,Sun Y,et al. Purification and identification of antioxidant peptides from walnut(Juglans regia L.)protein hydrolysates[J]. Peptides,2012,38(2):344-349.
    [6]
    杜倩,乌兰,刘睿,等. 核桃肽对幼年小鼠学习记忆能力的影响[J]. 中国生育健康杂志,2017,28(6):538-543.
    [7]
    Zhu N,Liu R,He L X,et al. Radioprotective effect of walnut oligopeptides against gamma radiation-induced splenocyte apoptosis and intestinal injury in mice[J]. Molecules(Basel,Switzerland),2019,24(8):1582.
    [8]
    Liu R,Wu L,Du Q,et al. Small molecule oligopeptides isolated from walnut(Juglans regia L.)and their anti-fatigue effects in mice[J]. Molecules(Basel,Switzerland),2018,24(1):pii:E45.
    [9]
    张亭,李迪,乌兰,等. 牡蛎低聚肽配伍核桃低聚肽和山药多糖对雄性小鼠性功能的影响[J]. 现代预防医学,2018,45(12):2141-2144

    ,2253.
    [10]
    Al-Sayed E,El-Naga R N. Protective role of ellagitannins from Eucalyptus citriodora against ethanol-induced gastric ulcer in rats:impact on oxidative stress,inflammation and calcitonin-gene related peptide[J]. Phytomedicine,2015,22(1):5-15.
    [11]
    Chauhan A K,Kang S C. Therapeutic potential and mechanism of thymol action against ethanol-induced gastric mucosal injury in rat model[J]. Alcohol(Fayetteville,NY),2015,49(7):739-745.
    [12]
    黄超. 金针菇多糖对运动疲劳大鼠T淋巴细胞转录因子T-bet、GATA-3基因及蛋白表达的影响[D]. 济南:山东体育学院,2014.
    [13]
    El-Maraghy S A,Rizk S M,Shahin N N. Gastroprotective effect of crocin in ethanol-induced gastric injury in rats[J]. Chemico-biological Interactions,2015,229:26-35.
    [14]
    Helmer K S,West S D,Shipley G L,et al. Gastric nitric oxide synthase expression during endotoxemia:Implications in mucosal defense in rats[J]. Gastroenterology,2002,123(1):173-186.
    [15]
    Lucetti L T,Silva R O,Santana A P,et al. Nitric oxide and hydrogen sulfide interact when modulating gastric physiological functions in rodents[J]. Digestive Diseases and Sciences,2017,62(1):93-104.
    [16]
    Wallace J L,Ianaro A,de Nucci G. Gaseous mediators in gastrointestinal mucosal defense and injury[J]. Digestive Diseases and Sciences,2017,62(9):2223-2230.
    [17]
    Brzozowska I,Konturek P C,Brzozowski T,et al. Role of prostaglandins,nitric oxide,sensory nerves and gastrin in acceleration of ulcer healing by melatonin and its precursor,L-tryptophan[J]. Journal of Pineal Research,2002,32(3):149-162.
    [18]
    Tu P S,Tung Y T,Lee W T,et al. Protective effect of camellia oil(Camellia oleifera Abel.)against ethanol-induced acute oxidative injury of the gastric mucosa in Mice[J]. Journal of Agricultural and Food Chemistry,2017,65(24):4932-4941.
    [19]
    Bhattacharyya A,Chattopadhyay R,Mitra S,et al. Oxidative stress:an essential factor in the pathogenesis of gastrointestinal mucosal diseases[J]. Physiological Reviews,2014,94(2):329-354.
    [20]
    Paulrayer A,Adithan A,Lee J H,et al. Aronia melanocarpa(black chokeberry)reduces ethanol-induced gastric damage via regulation of HSP-70,NF-kappaB,and MCP-1 signaling[J]. International Journal of Molecular Sciences,2017,18(6):1195.
    [21]
    Saeed Al-Wajeeh N,Halabi M F,Hajrezaie M,et al. The gastroprotective effect of vitex pubescens leaf extract against ethanol-provoked gastric mucosal damage in Sprague-Dawley rats[J]. PloS One,2016,11(9):e0157431.
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