Determination of Thirteen PFCs in Animal-derived Food by Improved QuEChERS Extraction Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

WANG Ying; DU Si-yu; ZHANG Hong; ZHANG Yu-hui; LI Ling; WANG Yan-hong; LI Guo-chen

doi:10.13386/j.issn1002-0306.2020030192

Volume 42 Issue 1

Dec. 2020

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Science and Technology of Food Industry > 2021 > 42(1): 239-249. > DOI: 10.13386/j.issn1002-0306.2020030192

WANG Ying, DU Si-yu, ZHANG Hong, ZHANG Yu-hui, LI Ling, WANG Yan-hong, LI Guo-chen. Determination of Thirteen PFCs in Animal-derived Food by Improved QuEChERS Extraction Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(1): 239-249. DOI: 10.13386/j.issn1002-0306.2020030192

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Determination of Thirteen PFCs in Animal-derived Food by Improved QuEChERS Extraction Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
2. Risk Assessment Laboratory on Environmental Factors of Agricultural Products Safety and Quality(Beijing), Ministry of Agriculture and Rural Affairs, Beijing 100029, China

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Received Date: March 15, 2020
Available Online: January 07, 2021

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Abstract

A method of ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-ESI-MS/MS)combined with improved QuEChERS was established for the determination of 13 perfluorinated compounds(PFCs)in animal-derived food. The samples were extracted with 0.2% hydrochloric acid acetonitrile and cleaned up with a sorbent mixture of N-propylethylenediamine(PSA),octadecyl bonded silica gel(C₁₈)and graphitized carbon black(GCB). The PFCs were separated by an Atlantis T3 reversed-phase column using gradient elution with a mixed solution of 2.5 mmol/L ammonium acetate methanol and 2.5 mmol/L ammonium acetate,and detected by electrospray ionization in negative mode using the multiple reaction monitoring(MRM)mode. The samples were quantified using isotope internal standard.The calibration curves of 13 PFCs were linear in the concentration range of 0.05~10 ng/mL. The limits of detection(LODs)were between 0.02 and 0.05 μg/kg,and the limits of quantitation(LOQs)were between 0.06 and 0.15 μg/kg. At the spiked levels of 0.2,1 and 2 μg/kg,the average recoveries were in the range of 62.3%~119.3%,with relative standard deviations(RSDs)of 3.5%~19.9%. With its simplicity,accuracy and high sensitivity,this method is suitable for the identification and quantification of 13 PFCs in animal-derived food.

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Science and Technology of Food Industry

Determination of Thirteen PFCs in Animal-derived Food by Improved QuEChERS Extraction Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry