Determination of Clotrimazole Residue in Animal-Derived Food by Gas Chromatography-Tandem Mass Spectrometry with Isotope Dilution

HAN Chao; HU Beizhen; LI Zhou; ZHU Zhen'ou; SHEN Yan; ZHOU Xiujin

doi:10.13386/j.issn1002-0306.2020030010

Volume 42 Issue 5

Feb. 2021

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Science and Technology of Food Industry > 2021 > 42(5): 216-220. > DOI: 10.13386/j.issn1002-0306.2020030010

HAN Chao, HU Beizhen, LI Zhou, ZHU Zhen'ou, SHEN Yan, ZHOU Xiujin. Determination of Clotrimazole Residue in Animal-Derived Food by Gas Chromatography-Tandem Mass Spectrometry with Isotope Dilution[J]. Science and Technology of Food Industry, 2021, 42(5): 216-220. DOI: 10.13386/j.issn1002-0306.2020030010

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Determination of Clotrimazole Residue in Animal-Derived Food by Gas Chromatography-Tandem Mass Spectrometry with Isotope Dilution

1. College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China;
2. Shaoxing Customs Comprehensive Technical Service Center, Shaoxing 312000, China;
3. Wenzhou Customs Comprehensive Technical Service Center, Wenzhou 325027, China;
4. Zhoushan Customs Comprehensive Technical Service Center, Zhoushan 316000, China

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Received Date: March 01, 2020
Available Online: March 02, 2021

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Abstract

Abstract

An isotope dilution gas chromatography-tandem mass spectrometry(GC-MS/MS)method for the determination of clotrimazole residues in animal-derived food(Pork,chicken,pig fat,pig heart,pig liver,pig kidney)was established. The samples were extracted with accelerated solvent extraction,and cleaned up by gel permeation chromatography. HP-5 MS chromatographic column(30 m×0.25 mm×0.25 μm)was used for separation,multi-reaction monitoring(MRM)mode detection,and isotope internal standard method for quantification. Results showed that there was a good linearity for clotrimazole in the range of 1~100 μg/L with a correlation coefficient(R²)of 0.9995. The limit of quantitation(LOQ,S/N≥10)of the method was 2.0 μg/kg. The average recoveries at three spiking levels(2.0,5.0,20.0 μg/kg)were 82.6%~116.5%. Relative standard deviation(RSD)were 5.2%~13.5%. This method has high sensitivity and accuracy,and can be used for the confirmation and quantitative analysis of clotrimazole residues in animal-originated foodstuffs.
- animal-derived food,
- clotrimazole,
- residue,
- isotope dilution,
- gas chromatography-tandem mass spectrometry

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