LI Wen-jie, WANG Chen-chen, YAN Meng-meng, LI Hui-dong, DING Rui-yan, FANG Li-ping, WANG Lan-zhi, HOU Yun-hua, YANG Qin-zheng, CHEN Zi-lei. Detection of Salbutamol by SPR Immunosensor Based on DMSA Self-assembled Monolayers[J]. Science and Technology of Food Industry, 2020, 41(22): 247-252. DOI: 10.13386/j.issn1002-0306.2020020112
Citation:
LI Wen-jie, WANG Chen-chen, YAN Meng-meng, LI Hui-dong, DING Rui-yan, FANG Li-ping, WANG Lan-zhi, HOU Yun-hua, YANG Qin-zheng, CHEN Zi-lei. Detection of Salbutamol by SPR Immunosensor Based on DMSA Self-assembled Monolayers[J]. Science and Technology of Food Industry, 2020, 41(22): 247-252. DOI: 10.13386/j.issn1002-0306.2020020112
LI Wen-jie, WANG Chen-chen, YAN Meng-meng, LI Hui-dong, DING Rui-yan, FANG Li-ping, WANG Lan-zhi, HOU Yun-hua, YANG Qin-zheng, CHEN Zi-lei. Detection of Salbutamol by SPR Immunosensor Based on DMSA Self-assembled Monolayers[J]. Science and Technology of Food Industry, 2020, 41(22): 247-252. DOI: 10.13386/j.issn1002-0306.2020020112
Citation:
LI Wen-jie, WANG Chen-chen, YAN Meng-meng, LI Hui-dong, DING Rui-yan, FANG Li-ping, WANG Lan-zhi, HOU Yun-hua, YANG Qin-zheng, CHEN Zi-lei. Detection of Salbutamol by SPR Immunosensor Based on DMSA Self-assembled Monolayers[J]. Science and Technology of Food Industry, 2020, 41(22): 247-252. DOI: 10.13386/j.issn1002-0306.2020020112
1. State Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250353, China;
2. Institution of Quality Standard and Testing Technology for Agro-Product, Shandong Academy of Agricultural Science, Jinan 250100, China;
3. Shandong Provincial Key Laboratory of Testing Technology for Food Quality and Security, Jinan 250100, China;
4. College of Life Science, Shandong Normal University, Jinan 250014, China
In this paper, dimercaptosuccinic acid (DMSA) was used as self-assembled monolayer to connect the antigen on the chip of surface plasmon resonance (SPR) immunosensor to construct DMSA-SPR immunosensor for detection of the model of salbutamol (SAL) and its detection effect was compared with that of traditional mercaptopropionic acid (MPA) -SPR sensor. The results showed that the recognition efficiency of antigen and antibody of DMSA-SPR immunosensor was nearly doubled at room temperature, the linear range was up to 5~150 ng/mL, and the limit of quantification (LOQ) was 5 ng/mL. The recovery rate of this sensor applied to SAL detection in pork reached 94.9%~108.0%, and had a good correlation with the analysis results of ultra-high phase liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. Therefore, this method would provide a new idea and method for improving the sensitivity of SPR immunosensor, and could be widely used in the detection of other small molecules.
本站查看
DownLoad: