Study on the Stability of (-)-Epigallocatechin-3-gallate

HAO Jing; YANG Tai-qin; WANG Han; WANG Xu; NI Juan

doi:10.13386/j.issn1002-0306.2020020035

Volume 42 Issue 1

Dec. 2020

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Science and Technology of Food Industry > 2021 > 42(1): 48-53. > DOI: 10.13386/j.issn1002-0306.2020020035

HAO Jing, YANG Tai-qin, WANG Han, WANG Xu, NI Juan. Study on the Stability of (-)-Epigallocatechin-3-gallate[J]. Science and Technology of Food Industry, 2021, 42(1): 48-53. DOI: 10.13386/j.issn1002-0306.2020020035

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Study on the Stability of (-)-Epigallocatechin-3-gallate

HAO Jing¹,
YANG Tai-qin^1,2,
WANG Han^1,2,
WANG Xu^1,2,
NI Juan^1,2, ,

1. College of Life Science of Yunnan Normal University, Kunming 650500, China;
2. Engineering Research Center for Sustainable Development and Utilization of Bioenergy of Yunnan Normal University, Kunming 650500, China

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Received Date: February 04, 2020
Available Online: January 07, 2021

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Abstract

Abstract

Objective: To analyze the environmental conditions of(-)-epigallocatechin-3-gallate(EGCG)oxidative polymerization and the effects of changes in its stability on the redox balance of the test cells,and to lay the foundation for an in-depth study of the role of EGCG in cell culture systems and living organisms. Methods: The research set different conditions of solution environment,EGCG concentration,temperature,time,pH,to evaluate the difference of EGCG oxidative polymerization under different conditions by detecting the change of OD₅₇₈ value. Result: The results showed that RPMI1640 and DMEM medium were more favorable for EGCG oxidation polymerization than H₂O. High temperature and high EGCG concentration and alkaline conditions promoted EGCG oxidation polymerization. At the same time,the oxidative polymerization of EGCG intensified with the extension of the reaction time. In a cell-containing culture system,EGCG also underwent auto-oxidative polymerization and produced H₂O₂,which increased the H₂O₂ concentration inside and outside the cell. However,the intracellular H₂O₂ concentration showed a trend of first increase and then decrease. Conclusion: Solution environment and pH changes are the key factors that cause EGCG oxidative polymerization. 20 μmol/L EGCG is more conducive to exert antioxidant activity through oxidative polymerization effect in cell culture.
- (-)-epigallocatechin-3-gallate,
- stability,
- oxidation,
- anti-oxidation

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References

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