HAO Jing, YANG Tai-qin, WANG Han, WANG Xu, NI Juan. Study on the Stability of (-)-Epigallocatechin-3-gallate[J]. Science and Technology of Food Industry, 2021, 42(1): 48-53. DOI: 10.13386/j.issn1002-0306.2020020035
Citation: HAO Jing, YANG Tai-qin, WANG Han, WANG Xu, NI Juan. Study on the Stability of (-)-Epigallocatechin-3-gallate[J]. Science and Technology of Food Industry, 2021, 42(1): 48-53. DOI: 10.13386/j.issn1002-0306.2020020035

Study on the Stability of (-)-Epigallocatechin-3-gallate

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  • Received Date: February 04, 2020
  • Available Online: January 07, 2021
  • Objective: To analyze the environmental conditions of(-)-epigallocatechin-3-gallate(EGCG)oxidative polymerization and the effects of changes in its stability on the redox balance of the test cells,and to lay the foundation for an in-depth study of the role of EGCG in cell culture systems and living organisms. Methods: The research set different conditions of solution environment,EGCG concentration,temperature,time,pH,to evaluate the difference of EGCG oxidative polymerization under different conditions by detecting the change of OD578 value. Result: The results showed that RPMI1640 and DMEM medium were more favorable for EGCG oxidation polymerization than H2O. High temperature and high EGCG concentration and alkaline conditions promoted EGCG oxidation polymerization. At the same time,the oxidative polymerization of EGCG intensified with the extension of the reaction time. In a cell-containing culture system,EGCG also underwent auto-oxidative polymerization and produced H2O2,which increased the H2O2 concentration inside and outside the cell. However,the intracellular H2O2 concentration showed a trend of first increase and then decrease. Conclusion: Solution environment and pH changes are the key factors that cause EGCG oxidative polymerization. 20 μmol/L EGCG is more conducive to exert antioxidant activity through oxidative polymerization effect in cell culture.
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