Science and Technology of Food Industry

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Volume 42 Issue 6
Mar.  2021
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LIU Danyu, ZHANG Yi, LIU Wei, YANG Chunyan. Recovery of Bovine Serum Albumin from Its Aqueous Solution by Ultrasonic Assisted Foam Separation[J]. Science and Technology of Food Industry, 2021, 42(6): 67-72,87. DOI: 10.13386/j.issn1002-0306.2020010050
Citation: LIU Danyu, ZHANG Yi, LIU Wei, YANG Chunyan. Recovery of Bovine Serum Albumin from Its Aqueous Solution by Ultrasonic Assisted Foam Separation[J]. Science and Technology of Food Industry, 2021, 42(6): 67-72,87. DOI: 10.13386/j.issn1002-0306.2020010050
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Recovery of Bovine Serum Albumin from Its Aqueous Solution by Ultrasonic Assisted Foam Separation

  • 1. School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300131, China;

  • 2. National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization, Hebei University of Technology, Tianjin 300131, China

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  • Received Date: January 06, 2020
  • Available Online: March 15, 2021
    Graphical Abstract
    • Abstract
  • In this work, a new method of ultrasonic assisted foam separation to effectively enrich bovine serum albumin(BSA) from its aqueous solution had been developed. Firstly, Fourier transform infrared spectroscopy(FTIR) was used to investigate the molecular structure changes of BSA before and after foam separation, and high performance size exclusion chromatography was used to determine the effect of ultrasonic wave on BSA aggregation. Then, the effects of liquid height, volumetric air flow rate and pore diameter of gas distributor on the efficiency of foam separation in the recovery of BSA were studied, respectively. The physicochemical properties of the recovered BSA were evaluated. The results indicated that the adsorption of BSA on the gas-liquid interface during foam separation would induce the generation of intermolecular disulfide bonds, and the BSA aggregation was effectually inhibited by ultrasonic wave. Under the suitable operation conditions of temperature 25℃, pH6.0, casein concentration 0.20 g/L, pore diameter of gas distributor 180 μm, liquid height 300 mm, feed feeding rate 2 mL/min, volumetric air flow rate 150 mL/min, ultrasonic power 600 W and ultrasonic treatment time 2 min, the enrichment ratio and recovery percentage of BSA were 6.3% and 85.3%, respectively. Furthermore, the surface hydrophobicity, foam properties and emulsification of BSA solution were significantly improved by ultrasonic treatment through inhibiting the production of BSA aggregation during the foam separation.
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  • [1]
    Kinoshita T,Nii S. Foam separation of metal ions and the potential ‘green’alternative to solvent extraction[J]. Solvent Extraction Research and Development,Japan,2012,19:1-15.
    [2]
    陈志刚,乔丹,许小红,等.泡沫分离法去除废水中的Cr(Ⅲ)离子[J]. 环境工程学报,2016,10(7):3623-3628.
    [3]
    Li R,Wu Z,Wang B Y,et al. Role of pH-induced structural change in protein aggregation in foam fractionation of bovine serum albumin[J]. Biotechnology Reports,2016,9:46-52.
    [4]
    Ouberai M M,Xu K,Welland M E. Effect of the interplay between protein and surface on the properties of adsorbed protein layers[J]. Biomaterials,2014,35(24):6157-6163.
    [5]
    Dickinson E. Adsorbed protein layers at fluid interfaces:Interactions,structure and surface rheology[J]. Colloids and Surfaces B:Biointerfaces,1999,15(2):161-176.
    [6]
    Dai L,Bergfreund J,Reichert C L,et al. Shear rheological properties of acid hydrolyzed insoluble proteins from Chlorella protothecoides at the oil-water interface[J]. Journal of Colloid and Interface Science,2019,551:297-304.
    [7]
    Li R,Ding L,Wu Z,et al. β-cyclodextrin assisted two-stage foam fractionation of bromelain from the crude extract of pineapple peels[J]. Industrial Crops and Products,2016,94:233-239.
    [8]
    Wang Y,Nan F,Zheng H,et al. Effects of temperature and trehalose on foam separation of nisin from the culture broth produced by Lactococcus lactis subspecies lactis W28[J]. Journal of Dairy Science,2012,95(10):5588-5596.
    [9]
    寇倩云,吴兆亮,胡楠. 牛血清白蛋白和溶菌酶混合溶液的泡沫性能与其浓度的关系[J]. 化工学报,2015,66(10):4107-4114.
    [10]
    樊继波,陈永斌,周洁. 白蛋白微球的制备方法及质量评价的研究进展[J]. 天津药学,2009,21(3):58-61.
    [11]
    王吟,熊扬,孙凤玲,等. 超声辅助高吸附型复合凝胶的制备及对水中Pb(Ⅱ)的响应和去除性能[J]. 物理化学学报,2016,32(10):2563-2573.
    [12]
    Jin J,Ma H,Wang B,et al. Effects and mechanism of dual-frequency power ultrasound on the molecular weight distribution of corn gluten meal hydrolysates[J]. Ultrasonics Sonochemistry,2016,30:44-51.
    [13]
    Zhang Y,Ma H,Wang B,et al. Effects of ultrasound pretreatment on the enzymolysis and structural characterization of wheat gluten[J]. Food Biophysics,2015,10(4):385-395.
    [14]
    Leong T,Johansson L,Juliano P,et al. Ultrasonic separation of particulate fluids in small and large scale systems:A review[J].Industrial & Engineering Chemistry Research,2013,52(47):16555-16576.
    [15]
    罗登林,丘泰球,卢群.超声波技术及应用(Ⅲ)——超声波在分离技术方面的应用[J]. 日用化学工业,2006,36(1):46-49.
    [16]
    Lin L,Cui H,He R,et al. Effect of ultrasonic treatment on the morphology of casein particles[J]. Ultrasonics Sonochemistry,2014,21(2):513-519.
    [17]
    杨晋辉,郑楠,杨永新,等.红外光谱在牛奶生产和检测方面的研究进展[J]. Transactions of the Chinese Society of Agricultural Engineering,2016,32(17).
    [18]
    周小玲,刘永乐,李向红,等.蛋白质谷氨酰胺酶对米谷蛋白的分子结构及功能性质的影响[J]. 中国食品学报,2010(5):98-105.
    [19]
    袁兴宇,冯文君,包小兰.脱酰胺对葵花籽蛋白酶解肽的钙结合量及体外消化性的影响[J].中国粮油学报,2017,32(10):73-77.
    [20]
    刘丹华,唐红芳,钱亚玲,等.高效液相色谱法在工作场所空气有毒物质测定中的应用[J].环境与职业医学,2017,34(4):362.
    [21]
    Tian S,Wu Z,Liu W,et al. Effective recovery of casein from its highly diluted solution by using a technology of foam fractionation coupled with isoelectric precipitation[J]. Journal of Food Engineering,2018,216:72-80.
    [22]
    Liu W,Wu Z,Wang Y,et al. Rhamnolipid assisted recovery of lycopene from the tomato-based processing wastewater using foam fractionation[J]. Journal of Food Engineering,2015,164:63-69.
    [23]
    Du L,Prokop A,Tanner R D. Effect of bubble size on foam fractionation of ovalbumin[M]. Biotechnology for Fuels and Chemicals. Humana Press,Totowa,NJ,2002:1075-1091.
    [24]
    Liu W,Zhang H X,Wu Z L,et al. Recovery of isoflavone aglycones from soy whey wastewater using foam fractionation and acidic hydrolysis[J]. Journal of Agricultural and Food Chemistry,2013,61(30):7366-7372.
    [25]
    Bos M A,van Vliet T. Interfacial rheological properties of adsorbed protein layers and surfactants:A review[J]. Advances in Colloid and Interface Science,2001,91(3):437-471.
    [26]
    Grassia P,Neethling S J,Cervantes C,et al. The growth,drainage and bursting of foams[J]. Colloids and Surfaces A:Physicochemical and Engineering Aspects,2006,274(1-3):110-124.
    [27]
    郭玲,刘爱国,胡志和,等.牛血清白蛋白的乳化和起泡特性[J]. 食品科学,2010(19):137-140.
    [28]
    Liu Y,Qiu S,Li J,et al. Peroxidase-mediated conjugation of corn fiber gum and bovine serum albumin to improve emulsifying properties[J]. Carbohydrate Polymers,2015,118:70-78.
    [29]
    Hou D,Lin D,Zhao C,et al. Control of protein(BSA)fouling by ultrasonic irradiation during membrane distillation process[J]. Separation and Purification Technology,2017,175:287-297.
    [30]
    章开,刘丽娟.超声波处理对牛血清白蛋白结构和乳化性的影响[J]. 食品工业科技,2017,38(4):116-120.
    [31]
    Zhang F,Wu Z,Yin H,et al. Effect of ionic strength on the foam fractionation of bsa with existence of antifoaming agent[J]. Chemical Engineering and Processing:Process Intensification,2010,49(10):1084-1088.
    [32]
    Yang X,Li Y,Li S,et al. Effects of ultrasound-assisted α-amylase degradation treatment with multiple modes on the extraction of rice protein[J]. Ultrasonics Sonochemistry,2018,40:890-899.
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