Visualization LAMP Detection Method of Living Staphylococcus aureus in Livestock and Poultry Meat

Objective:To establish a fast detection method with simple equipment requirements and visual results for Staphylococcus aureus in livestock and poultry meat. Methods:A visualized method of PMA-HNB-LAMP for rapid detection of Staphylococcus aureus in livestock and poultry meat was established by optimizing the concentration of HNB,PMA. In this study,the specificity,sensitivity,simulated pollution experiment and practical application effect were tested. Results:A LAMP amplification system was successfully established. By adding 210 μmol/L HNB to the LAMP amplification system,significant negative and positive results could be obtained. The HNB-LAMP method established in this study had strong specificity,the sensitivity of the pure bacterial solution of this method was 10⁴ CFU/mL,and the sensitivity of the sample matrix was 10⁵ CFU/mL.Through the optimization of PMA concentration and processing conditions,it was determined to use PMA of 10 μg/mL to treat samples by incubating in darkness for 5 min and exposing for 15 min at the room temperature. The results of PMA-HNB-LAMP and traditional microbiological methods were consistent in the detection of artificially polluted samples. Conclusions:A PMA-HNB-LAMP method for the detection of living Staphylococcus aureus in livestock and poultry meat was successfully established in this research.